Journal of the Optical Society of Korea

Optical Society of Korea (한국광학회)
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Particle Image Velocimetry of the Blood Flow in a Micro-channel Using the Confocal Laser Scanning Microscope

  • Kim, Wi-Han (School of Mechanical Engineering, Kyungpook National University) ;
  • Kim, Chan-Il (School of Mechanical Engineering, Kyungpook National University) ;
  • Lee, Sang-Won (School of Mechanical Engineering, Kyungpook National University) ;
  • Lim, Soo-Hee (School of Mechanical Engineering, Kyungpook National University) ;
  • Park, Cheol-Woo (School of Mechanical Engineering, Kyungpook National University) ;
  • Lee, Ho (School of Mechanical Engineering, Kyungpook National University) ;
  • Park, Min-Kyu (School of Mechanical and Automotive Engineering Technology, Yeungnam College of Science & Technology)
  • Received : 2010.01.06
  • Accepted : 2010.02.19
  • Published : 2010.03.25
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Abstract

We used video-rate Confocal Laser Scanning Microscopy (CLSM) to observe the motion of blood cells in a micro-channel. Video-rate CLSM allowed us to acquire images at the rate of 30 frames per second. The acquired images were used to perform Particle Image Velocimetry (PIV), thus providing the velocity profile of the blood in a micro-channel. While previous confocal microscopy-assisted PIV required exogenous micro/nano particles as the tracing particles, we employed blood cells as tracing particles for the CLSM in the reflection mode, which uses light back-scattered from the sample. The blood flow at various depths of the micro-channel was observed by adjusting the image plane of the microscope. The velocity profile at different depths of the channel was measured. The confocal micro-PIV technique used in the study was able to measure blood velocity up to a few hundreds ${\mu}m/sec$, equivalent to the blood velocity in the capillaries of a live animal. It is expected that the technique presented can be applied for in vivo blood flow measurement in the capillaries of live animals.

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References

  1. D. N. Ku, “Blood flow in arteries,” Annu. Rev. Fluid. Mech. 29, 399-434 (1997). https://doi.org/10.1146/annurev.fluid.29.1.399
  2. L. Dintenfass, “Blood rheology in pathogenesis of the coronary heart disease,” Am. Heart J. 77, 139-147 (1969). https://doi.org/10.1016/0002-8703(69)90139-2
  3. E. Fossum, A. Hoieggen, and A. Moan, “Whole blood viscosity, blood pressure and cardiovascular risk factors in healthy blood donors,” Blood Pressure 6, 161-165 (1997). https://doi.org/10.3109/08037059709061932
  4. A. J. Lee, P. I. Mowbray, and G. D. Lowe, “Blood viscosity and evaluated carotid intima-media thickness in men and women,” Circulation 97, 1467-1473 (1998). https://doi.org/10.1161/01.CIR.97.15.1467
  5. K. Toth, G. Kesmarky, and J. Vekasi, “Hemorheological and hemodynamic parameters in patients with essential hypertension,” Clin. Hemorheol. Microcirc. 21, 209-216 (1999).
  6. K. L. Resch, E. Ernst, A. Matrai, and H. F. Paulsen, “Fibrinogen and viscosity as risk factors for subsequent cardiovascular event in stroke survivors,” Ann. Intern. Med. 117, 371-375 (1992). https://doi.org/10.7326/0003-4819-117-5-371
  7. J. R. Haywood, R. A. Shaffer, C. Fastenow, G. D. Fink, and M. J. Brody, “Regional blood flow measurement with pulsed Doppler flowmeter in conscious rat,” Am. J. Physiol. Heart Circ. Physiol. 241, H273-H278 (1981). https://doi.org/10.1152/ajpheart.1981.241.2.H273
  8. H. Wayland and P. C. Johnson, “Erythrocyte velocity measurement in microvessels by a two-slit photometric method,” J. Appl. Physiol. 22, 333-337 (1967). https://doi.org/10.1152/jappl.1967.22.2.333
  9. T. Cochrane, J. C. Earnshaw, and A. H. G. Love, “Laser Doppler measurement of blood velocity in microvessels,” Med. Biol. Eng. Comput. 19, 589-596 (1981). https://doi.org/10.1007/BF02442773
  10. J. Seki, Y. Sasaki, T. Oyama, and J. Yamamoto, “Fiber-optic laser-Doppler anemometer microscope applied to the cerebral microcirculation in rats,” Biorh. 33, 463-470 (1996). https://doi.org/10.1016/S0006-355X(97)00034-6
  11. Y. Zhao, Z. Chen, C. Saxer, S. Xiang, J. F. de Boer, and J. S. Nelson, “Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity,” Opt. Lett. 25, 114-116 (2000). https://doi.org/10.1364/OL.25.000114
  12. Z. Chen, T. E. Milner, S. Srinivas, X. Wang, and A. Malekafzali, “Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography,” Opt. Lett. 22, 1119-1121 (1997). https://doi.org/10.1364/OL.22.001119
  13. Y.-C. Ahn, W. Jung, and Z. Chen, “Optical sectioning for microfluidics: secondary flow and mixing in a meandering microchannel,” Lab. Chip. 8, 125-133 (2008). https://doi.org/10.1039/b713626a
  14. R. J. Adrian, “Particle-imaging techniques for experimental fluid mechanics,” Annu. Rev. Fluid. Mech. 23, 261-304 (1991). https://doi.org/10.1146/annurev.fl.23.010191.001401
  15. C. D. Meinhart, S. T. Wereley, and J. G. Santiago, “PIV measurements of a microchannel flow,” Exp. Fluids. 27, 414-419 (1999). https://doi.org/10.1007/s003480050366
  16. M. R. Brown, J. M. Macinnes, and R. W. K. Allen, “Micro-PIV simulation and measurement in complex microchannel geometries,” Meas. Sci. Technol. 16, 619-626 (2005). https://doi.org/10.1088/0957-0233/16/3/002
  17. R. Okuda, Y. Sugii, and K. Okamoto, “Velocity measurement of blood flow in a microtube using micro PIV system,” in Proc. PSFVIP-4 (Chamonix, France, Jun. 2003), pp. 1-7.
  18. M. Minsky, “Microscopy apparatus,” US patent 3013467 (1961).
  19. T. Wilson, ed., Confocal Microscopy (Academic Press, San Diego, USA, 1990).
  20. J. S. Park, C. K. Choi, and K. D. Kihm, “Optically sliced micro-PIV using confocal laser scanning microscopy (CLSM),” Exp. Fluids. 37, 105-119 (2004). https://doi.org/10.1007/s00348-004-0790-6
  21. R. Lima, S. Wada, K. Tsubota, and T. Yamaguchi, “Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square micro channel,” Meas. Sci. Technol. 17, 797-808 (2006). https://doi.org/10.1088/0957-0233/17/4/026
  22. I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with multimodal video rate microscopy,” IEEE J. Select. Topics Quantum Electron. : Special Issue on Biophotonics 14, 10-18 (2008). https://doi.org/10.1109/JSTQE.2007.912751
  23. C. W. Park, S. J. Lee, and S. Shin, “Micro-PIV measurements of in vitro blood flow in a micro-channel,” International Journal of Vascular Biomedical Engineering 1, 30-33 (2003).

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