Journal of dental hygiene science (치위생과학회지)

The Korean Society of Dental Hygiene Science (한국치위생과학회)
권호 표지

MAPK Signal Pathways in Regulation of Odontoblastic Differentiation by Induction of HO-1 in Human Dental Pulp Cells

MAPK 경로를 통한 HO-1과 분화 표지자 발현

  • Kim, Sun-Ju (Dept. of Dental Hygiene, Cheongju University)
  • 김선주 (청주대학교 치위생학과)
  • Received : 2010.06.29
  • Accepted : 2010.08.18
  • Published : 2010.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to examine the MAPK signaling pathways involved in regulation of HO-1 and the odontoblast differentiation markers during the odontoblastic differentiation for HDPCs. We evaluated cell growth by MTT assay and differentiation marker mRNA expression by RT-PCR. When the cells were treated with p38 inhibitor (SB203580, $10{\mu}M$), JNK inhibitor (SP600125, $10{\mu}M$), and ERK inhibitor (PD98059, $20{\mu}M$) for 7 days, cell growth and expression of HO-1 and differentiation makers were significantly decreased in HDPCs. Our results suggest that odontoblastic differentiation is positively regulated by HO-1 induction in HDPCs via ERK, JNK, and p38 signaling pathways. Thus, pharmacological HO-1 induction might represent a potent therapeutic approach for pulp capping and the regeneration of HDPCs.

Keywords

References

  1. Zhang C et al.: Inhibition of human dental pulp stem cell differentiation by Notch signaling. J Dent Res 87(3): 250-5, 2008. https://doi.org/10.1177/154405910808700312
  2. Gronthos S et al.: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 97(25): 13625-30, 2000. https://doi.org/10.1073/pnas.240309797
  3. Ruch, J.V: Odontoblast commitment and differentiation. Biochem Cell Biol. 76: 923-938, 1998. https://doi.org/10.1139/o99-008
  4. Hideki Agata et al.: Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells. Differentiation 76: 981-993, 2008.
  5. Butler WT, Ritchie H: The nature and functional significance of dentin extracellular matrix proteins. Int. J. Dev. Biol 39: 169-79, 1995.
  6. Bae HS, Cho YS: The effect of over-expression and inactivation of nuclear factor I-C on the dentin matrix gene expression of MDPC-23 odontoblasts. J Dent Hyg Sci 9(4):427-433, 2009.
  7. Papaccio G et al.: Long-term cryopreservation of dental pulp stem cells(SBD-DPSCs) and their differentiated osteoblasts: a cell source for tissue repair. J Cell Physiol 208: 319-325, 2006. https://doi.org/10.1002/jcp.20667
  8. Schwer CI et al.: Heme oxygenase-1 inhibits the proliferation of pancreatic stellate cells by repression of the extracellular signal-regulated kinase1/2 pathway. J Pharmacol Exp Ther 327(3): 863-71, 2008. https://doi.org/10.1124/jpet.108.136549
  9. Lee SK et al.: Nitric oxide modulates osteoblastic differentiation with heme oxygenase-1 via the mitogen activated protein kinase and nuclear factor-kappaB pathways in human periodontal ligament cells. Biol Pharm Bull 32(8): 1328-34, 2009. https://doi.org/10.1248/bpb.32.1328
  10. Durante W: Heme oxygenase-1 in growth control and its clinical application to vascular disease. J Cell Physiol 195(3): 373-82, 2003. https://doi.org/10.1002/jcp.10274
  11. Lee SK et al.: Mechanical stress activates proinflammatory cytokines and antioxidant defense enzymes in human dental pulp cells. J Endod 34: 1364-9, 2008. https://doi.org/10.1016/j.joen.2008.08.024
  12. Barbagallo I et al.: A cytoprotective role for the heme oxygenase-1/CO pathway during neural differentiation of human mesenchymal stem cells. J Neurosci Res 86: 1927-35, 2008. https://doi.org/10.1002/jnr.21660
  13. Stephan Immenschuh, Giuliano Ramadori: Gene regulation of heme oxygenase-1 as a therapeutic target. Biochem Pharmacol 60(8): 1121-1128, 2000. https://doi.org/10.1016/S0006-2952(00)00443-3
  14. Wu WT et al.: Proteasome inhibitors up-regulate haem oxygenase-1 gene expression: requirement of p38 MAPK (mitogen-activated protein kinase) activation but not of NFkappa B (nuclear factor kappa B) inhibition. Biochem J 379(3): 587-93, 2004. https://doi.org/10.1042/BJ20031579
  15. Kim SJ et al.: The role of Heme Oxyganase-1 on proliferation and differentiation in human dental pulp cells. J Endod 36(8): 1326-31, 2010. https://doi.org/10.1016/j.joen.2010.04.011
  16. Farombi EO, Surh YJ: Heme oxygenase-1 as a potential therapeutic target for hepatoprotection. J Biochem Mol Biol 30:39(5): 479-91, 2006. https://doi.org/10.5483/BMBRep.2006.39.5.479
  17. Zhang W, Liu HT: MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res 12(1): 9-18, 2002. https://doi.org/10.1038/sj.cr.7290105
  18. Krishna M, Narang H: The complexity of mitogen-activated protein kinases (MAPKs) made simple. Cell Mol Life Sci 65(22): 3525-44, 2008. https://doi.org/10.1007/s00018-008-8170-7
  19. Lee HJ et al.: Differential induction of heme oxygenase-1 against nicotine-induced cytotoxicity via the PI3K, MAPK, and NF-kappa B pathways in immortalized and malignant human oral keratinocytes. J Oral Pathol Med 37(5): 278-86, 2008. https://doi.org/10.1111/j.1600-0714.2007.00616.x
  20. Ana Cuadrado et al.: A new p38 MAP kinase-regulated transcriptional coactivator that stimulates p53-dependent apoptosis. EMBO J 26(8): 2115-2126, 2007. https://doi.org/10.1038/sj.emboj.7601657
  21. Kitagawa M et al.: Immortalization and characterization of human dental pulp cells with odontoblastic differentiation. Arch Oral Biol 52: 727-31, 2007. https://doi.org/10.1016/j.archoralbio.2007.02.006
  22. Hayami T et al.: Dexamethasone's enhancement of osteoblastic markers in human periodontal ligament cells is associated with inhibition of collagenase expression. Bone 40: 93-104, 2007. https://doi.org/10.1016/j.bone.2006.07.003
  23. Couble ML et al.: Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif Tissue Int 66: 129-138, 2000. https://doi.org/10.1007/PL00005833
  24. Nakashima M: Bone morphogenetic proteins in dentin regeneration for potential use in endodontic therapy. Cytokine Growth Factor Rev 163: 369-376, 2005.
  25. Shi S et al.: The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res 8: 191-199, 2005. https://doi.org/10.1111/j.1601-6343.2005.00331.x
  26. Butler WT: Dentin matrix proteins and dentinogenesis. Connect Tissue Res 33: 59-65, 1995. https://doi.org/10.3109/03008209509016983
  27. Nakamura Y et al.: Immunohistochemical characterization of rapid dentin formation induced by enamel matrix derivative. Calcif Tissue Int 75(3): 243-52, 2004. https://doi.org/10.1007/s00223-003-0153-y
  28. Ibaraki K et al.: Bone matrix mRNA expression in differentiating fetal bovine osteoblasts. J Bone Miner Res 7: 743-754, 1992.
  29. Feng JQ et al.: The Dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. J Dent Res 82(10): 776-80, 2003. https://doi.org/10.1177/154405910308201003
  30. Otterbein LE, Choi AM: Heme oxygenase: colors of defense against cellular stress. Am J Physiol Lung Cell Mol Physiol 279(6): 1029-37, 2006.
  31. Kook YA et al.: Effects of substance P on osteoblastic differentiation and heme oxygenase-1 in human periodontal ligament cells. Cell Biol Int 33: 424-428, 2009. https://doi.org/10.1016/j.cellbi.2008.12.007
  32. Reibmant et al.: Airway epithelial cells release MIP-3alpha/ CCL20 in response to cytokines and ambient particulate matter. Am J Respir Cell Mol Biol 28(6): 648-54, 2003. https://doi.org/10.1165/rcmb.2002-0095OC
  33. Immenschuh S, Ramadori G: Gene regulation of heme oxygenase-1 as a therapeutic target. Biochem Pharmacol 60(8): 1121-1128, 2000. https://doi.org/10.1016/S0006-2952(00)00443-3