Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Hericium erinaceus Mycelia Supplementation on the Oxidative Stress and Inflammation Processes Stimulated by LPS and Their Mechanisms in BALB/C Mice

BALB/C Mice에서 노루궁뎅이버섯 균사체 보충이 LPS로 인한 산화적 스트레스와 염증 반응에 미치는 효과 및 기전

  • Jang, Ji-Hyun (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Noh, Kyung-Hee (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Choi, Ji-Na (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Jin, Kyong-Suk (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Shin, Jin-Hyuk (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • On, Joon-Ho (KBF Co., LTD) ;
  • Cho, Chung-Won (School of Biological Sciences, College of Biomedicinal Sciences and Engineering, Inje University) ;
  • Jeong, Woo-Sik (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Kim, Myo-Jeong (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Song, Young-Sun (BK21 Center of Smart Foods and Drugs, Food Science Institute, and Biohealth Product Research Center, Inje University)
  • 장지현 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터) ;
  • 노경희 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터) ;
  • 최지나 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터) ;
  • 진경숙 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터) ;
  • 신진혁 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터) ;
  • 온준호 (KBF(주)) ;
  • 조정원 (인제대학교 의생명과학대학 생명과학부) ;
  • 정우식 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터) ;
  • 김묘정 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터) ;
  • 송영선 (인제대학교 BK21 식의약생명공학과, 식품과학연구소 및 바이오헬스 소재 연구센터)
  • Published : 2010.02.27
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Abstract

This study was carried out to investigate the anti-oxidative and anti-inflammatory actions of Hericium erinaceus mycelia in BALB/C mice injected with lopopolysaccharide (LPS), called endotoxin. Mice (6 weeks of age) weighing approximately $24.73\pm0.11$ g were divided into 5 groups and were fed on the experimental diets containing Hericium erinaceus mycelia powder (HMP) for 1 week. Experimental groups were NC (normal control), HMP-C (HMP control), LC (LPS control), HMP 3%, and HMP 10%. Endotoxin shock was induced by intraperitoneal injection of LPS (100 mg/kg BW). NC and HMP-C groups were injected with saline solution (100 mg/kg BW). Food efficiency ratio were significantly (p<0.05) decreased in the HMP supplementation groups. Total fat and $\beta$-glucan excretion were higher in HMP supplementation groups than NC and LC groups, while plasma TG level was not different among groups. Plasma ALT levels were significantly (p<0.05) lower in the HMP supplementation groups than in LC group at 8 hr after LPS injection, while tumor necrosis factor-$\alpha$ and interleukine-6 levels of plasma were not different among groups. Hepatic superoxide dismutase, glutathione-reductase (GSH-red), and glutathione-peroxidase activities were higher in the HMP supplementation groups than in LC group at 4 hr after intraperitoneal injection of LPS. Hepatic GSH levels and protein expression of GSH-red was significantly (p<0.05) higher in the HMP supplemented groups than in LC group at 1 hr, 4 hr and 8 hr after LPS injection. From the above results, it is concluded that Hericium erinaceus mycelia may ameliorate hepatic oxidative stress by LPS through the elevation of hepatic glutathione level and antioxidant enzyme activities, which support the hepatoprotective effect of Hericium erinaceus mycelia.

본 연구는 in vivo에서의 6주령의 BALB/C 생쥐(평균체중 $24.73\pm0.11$ g)에게 1주일 간 노루궁뎅이버섯 균사체(Hericium erinaceus mycelia)를 첨가한 식이를 급여한 후 내독소인 LPS로 인한 산화적 스트레스 및 염증반응에 미치는 영향을 조사하고자 하였다. 실험군은 5군으로 분류하였으며 정상 대조군(NC)과 양성 대조군(LC)은 chow를, HMP 10%군과 HMP 3%군은 chow에 노루궁뎅이버섯 균사체를 각각 3%와 10%를 첨가하였으며 HMP 대조군(HMP-C)은 chow에 노루궁뎅이버섯 균사체를 10% 첨가하여 pellet 형태로 조제하여 1주일 간 각각의 실험 식이를 급여하였다. LC군과 HMP 3% 및 HMP 10%군은 각각 LPS(100 mg/kg BW)를 투여하였으며 NC군과 HMP-C군은 생리식염수를 LPS와 동일한 농도로 투여하였다. 실험동물의 식이효율은 노루궁뎅이버섯 균사체를 보충한 군에서 저하되었으며 노루궁뎅이버섯 균사체의 보충군에서 체중감소 효과를 보였다. 노루궁뎅이버섯 균사체 10%를 보충한 HMP-C군과 HMP 10%군은 분변의 배설량이 가장 많았고 분변 중 총지방 양은 노루궁뎅이버섯 균사체를 섭취한 군에서 높은 수준을 보였으나 섭취량에 따른 현저한 차이는 보이지 않았고, 혈장 TG 함량은 군 간에 유의적인 차이는 보이지 않았으나 노루궁뎅이버섯 균사체 10%를 첨가한 식이를 섭취한 군에서 다소 높은 경향을 보였다. 분변 100 g 당 $\beta$-glucan의 함량은 노루궁뎅이버섯 균사체 3%를 보충한 군에서 가장 높았다. 혈장의 ALT 수준은 LPS를 투여한 후 8시간 경과 후에는 LC군에서 현저하게 증가한 반면 노루궁뎅이버섯 균사체를 첨가한 식이를 급여한 HMP 3%와 HMP 10%군에서는 유의적으로(p<0.05) 저하되어 NC군과 비슷한 수준을 보였다. 간에서의 SOD, GSH-red, GSH-px 활성은 LPS 투여 후 LC군에 비해 노루궁뎅이버섯 균사체를 섭취한 군에서 증가하였으며 GSH-red의 단백질 발현과 GSH 함량 또한 높아지는 것으로 나타났다. 이상의 결과로 미루어 볼 때 노루궁뎅이버섯 균사체의 보충은 내독소인 LPS로 유도된 산화적 스트레스에 저항하여 간 기능을 보호하는 효과가 있는 것으로 보이며 그 기전으로는 항산화 효소계 활성과 GSH-red 단백질 발현 증가, 그리고 GSH 수준 향상 등을 열거할 수 있다.

Keywords

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