Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant and Antitumor Activities of Ethanol Extracts from Unhulled and Hulled Rice Hiami (Oryza sativa L. cv. Hiami)

하이아미의 정조 및 현미 추출물의 in vitro 항산화 및 항암활성

  • Woo, Koan-Sik (National Institute of Crop Science, Rural Development Administration) ;
  • Chun, A-Reum (National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Sea-Kwan (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Kee-Jong (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Dae-Jung (National Institute of Crop Science, Rural Development Administration) ;
  • Yang, Chang-Ihn (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Yeon-Gyu (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Jae-Hyun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 우관식 (농촌진흥청 국립식량과학원) ;
  • 천아름 (농촌진흥청 국립식량과학원) ;
  • 오세관 (농촌진흥청 국립식량과학원) ;
  • 김기종 (농촌진흥청 국립식량과학원) ;
  • 김대중 (농촌진흥청 국립식량과학원) ;
  • 양창인 (농촌진흥청 국립식량과학원) ;
  • 김연규 (농촌진흥청 국립식량과학원) ;
  • 김재현 (국립농업과학원) ;
  • 정헌상 (충북대학교 식품공학과)
  • Published : 2010.02.27
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Abstract

To evaluate the antioxidant and antitumor potential of a newly bred rice, Hiami (Suweon-511), total polyphenol content, $\gamma$-oryzanol content, radical scavenging activities, and antitumor activities were measured (control: Ilpum). Total polyphenol contents were 6.31 and 3.75 mg/g for unhulled (UHR) and hulled rice (HR) of Hiami, which was higher than that of Ilpum (5.66 and 3.47 mg/g). The $\gamma$-oryzanol contents were 33.53 and 39.47 mg/100 g for UHR and HR of Hiami, which was higher than that of Ilpum (24.33 and 28.68 mg/100 g). DPPH radical, hydrogen peroxide, superoxide radical and hydroxyl radical scavenging activities of 70% ethanol extracts of Hiami were higher compared to Ilpum, and UHR was higher than those of HR. Antitumor activities of the 70% ethanol extracts of Hiami and Ilpum were increased with a dose-dependent manner. The extracts of Hiami have higher activities of antitumor activities on gastric and breast cancer cell lines compared to other cancer cell lines, and Ilpum has higher activities of antitumor activities on colon and liver cancer cell lines.

하이아미의 정조와 현미상태에 대해 일품벼를 대조구로 하여 항산화성분의 함량과 에탄올 추출물에 대한 in vitro 항산화활성 및 항암활성을 측정하였다. 하이아미의 정조 및 현미 추출물의 총 폴리페놀 함량은 각각 6.31 및 3.75 mg/g으로 나타났으며, 일품은 각각 5.66 및 3.47 mg/g으로 나타나 하이아미가 약간 높은 것을 알 수 있었다. 하이아미의 정조와 현미 추출물의 $\gamma$-oryzanol 함량은 각각 33.53 및 39.47 mg/100 g으로 나타났으며, 일품의 경우 각각 24.33 및 28.68 mg/100 g으로 나타나 일품에 비해 하이아미에서 높은 함량을 보이는 것으로 나타났다. 하이아미와 일품벼 정조 및 현미의 70% 에탄올 추출물에 대한 DPPH radical, hydrogen peroxide, superoxide radical 및 hydroxyl radical 소거활성을 5 mg/mL의 농도에서 측정한 결과 하이아미가 일품에 비해 높은 것으로 나타났으며, 정조 추출물이 현미 추출물보다 높은 radical 소거활성을 보이는 것으로 나타났다. 70% 에탄올 추출물에 대한 in vitro 항암활성을 측정한 결과 정조 및 현미 추출물의 억제 활성은 용량 의존적으로 증가하는 것으로 나타났으며, 특히 하이아미는 위암 및 유방암세포에 높은 활성을 보였으며, 일품은 대장암 및 간암세포에 대해 높은 활성을 보였다.

Keywords

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