The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Tyrosinase Inhibitory Activity of Macrolepiota procera

큰갓버섯(Macrolepiota procera)의 Tyrosinase 효소활성 저해효과

  • 곽정훈 (동국대학교 대학원 생명과학과) ;
  • 한영환 (동국대학교 대학원 생명과학과)
  • Received : 2010.11.30
  • Accepted : 2010.12.20
  • Published : 2010.12.31
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Abstract

Inhibitory effect on tyrosinase activity and melanogenesis of the mycelia and mycelial culture broth of Macrolepiota procera were investigated. The methanol fraction of culture broth showed 56.6% of tyrosinase inhibitory activity and its IC50 was 8.78 mg/ml. Using Streptococcus bikiniensis for melanogenesis in vitro, the methanol extract of mycelia showed 94.0% inhibition of melanogenesis.

멜라닌 생합성에 관련된 tyrosinase 효소활성의 억제를 측정을 위하여, 큰갓버섯(Macrolepiota procera) 균사체 및 균사체 배양액을 이용하여 실험을 하였다. 배양액의 메탄올 분획이 56.6%의 효소활성 억제를 보여주었으며, 농도 의존적억제 실험에서 50%의 tyrosinase 효소활성 억제 농도는 8.78mg/ml이었다. Streptococcus bikiniensis를 이용한 멜라닌생성 억제는 균사체의 메탄올 추출물에서 94.0%의 우수한 결과를 나타내었다(Table 2).

Keywords

References

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