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밀리타리스 동충하초 열수추출물의 멜라닌 분비 억제능 효과

Inhibitory Effects of Melanin Secretion on B16 Melanoma cell of Cordyceps militaris Water Extract

  • 남병혁 (부산대학교 자연과학대학 미생물학과) ;
  • 조월순 (동남권원자력의학원) ;
  • 최유진 (동남권원자력의학원) ;
  • 이재윤 (부산대학교 자연과학대학 미생물학과) ;
  • 강은영 ((재)부산테크노파크 해양생물산업육성센터) ;
  • 정민호 (동아대학교 의과대학 미생물학교실) ;
  • 이재동 (부산대학교 자연과학대학 미생물학과)
  • Nam, Byung-Hyouk (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Jo, Wool-Soon (Dong Nam Institute of Radiological & Medical Sciences) ;
  • Choi, Yoo-Jin (Dong Nam Institute of Radiological & Medical Sciences) ;
  • Lee, Jae-Yun (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Kang, Eun-Young (Busan Techno-park Marine Bio-Industry Development Center) ;
  • Jeong, Min-Ho (Department of Microbiology, Dong-A University Medical College) ;
  • Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
  • 투고 : 2010.07.21
  • 심사 : 2010.11.17
  • 발행 : 2010.12.31

초록

The present study aims to evaluate Cordyceps militaris water extract (CMWE) with a view to develop melanogenesis inhibitors. Inhibitory activities of CMWE against tyrosinase, L-DOPA(L-3,4-dihydroxyphenylalanine) oxidation, and melanin biosynthesis in B16 mouse melanoma cells were investigated. CMWE, at $5000\;{\mu}g/ml$, inhibited tyrosinase activity of 71% and DOPA oxidation of 40% as reacting with L-DOPA. Furthermore, B16 mouse melanoma cell survived over 50% from low to high dose on MTT assay, and CMWE markedly inhibited (> 50%) melanin synthesis at $5000\;{\mu}g/ml$. The inhibitory effect of CMWE on melanogenesis was attributed to enhancement of tyrosinase degradation. Key enzyme of melanin biosynthesis is tyrosinase which catalyses a beginning step from tyrosine to DOPA quinine and melanin formation step, respectively. These results indicated that CMWE may be a potential source of novel whitening agents for cosmetic or therapeutic application.

키워드

참고문헌

  1. Aburjai, T. and Natsheh, F. M. 2003. Plants used in cosmetics. Phytotherapy Res. 17(9):987-1000. https://doi.org/10.1002/ptr.1363
  2. Agar, N. and Young, A. R. 2005. Melanogenesis : a photoprotective response to DNA damage. Mutation Res. 571(1):121-132. https://doi.org/10.1016/j.mrfmmm.2004.11.016
  3. Bae, S. Y. and Keun, L. J. 1998. A bibliographical study on the origin of Chinese Caterpillar Fungus, Kor. J. Herbology, 13(2):181-187.
  4. Briganti, S., Camera, E. and Picardo, M. 2003. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res. 16(2):101-110. https://doi.org/10.1034/j.1600-0749.2003.00029.x
  5. Cabanes, J., Chazarra, S. and Garcia-Carmona, F. 1994. Kojic acid, a cosmetic skin whitening agent, is a show binding inhibitor of cathecilase activity of tyrosinase. J. Pharmacol. 46(12):982-985. https://doi.org/10.1111/j.2042-7158.1994.tb03253.x
  6. Choi, W. H., Ann, H. S., Choi, T. Y., Jin, S. Y. and Ahn, R.M. 2006. Effects of natura extracts on UVB-induced pigmentation and inflammation in C57BL/6 mouse skin. Kor. J. Env. Health Sci. 32(5):492-498.
  7. Hearing, V. J. and Jimenez, M. 1987. Mammalian tyrosinase-the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19:1141. https://doi.org/10.1016/0020-711X(87)90095-4
  8. Hearing, V. J. and Jimenez, M. 1989. Analysis of mammalian pigmentation at the molecular level. Pigment Cell Res. 2:75. https://doi.org/10.1111/j.1600-0749.1989.tb00166.x
  9. Jo, J. S. 1998. Study on the origin of Chinese caterpillar fungus. J. East-West Medicines 671-680.
  10. Kim, H. S., Roh, Y. J. and Choe, M. 2005. Cordyceps militaris increases hepatic glucokinase activities. J. Kor. Soc. Food Sci. Nutr. 34(2):158-161. https://doi.org/10.3746/jkfn.2005.34.2.158
  11. Kim, J. H., Cho, M. R., Ryu, C. R. and Chae, W. S. 2002. Effect of Cordyceps militaris mycelia(CMM) oral administration and herbal acupuncture at BL13, LU4 on asthma induced by ovalbumin in rats. J. Kor. Acupuncture & Moxibustion Soc. 19(2):39-50.
  12. Kim, M. N., Oh, S. W., Lee, D. S. and Ham, S. S. 2001. Antioxidative and antimutagenic effect of the ethaol extract from Cordyceps militaris. Kor J. Postharvest Sci. Technol. 8(1):109-117.
  13. Kim, S. J., Lim, D. K., Park, C. W., Cerbo, R. M., Hyung, S. W., Lee, K. K., Kim, J. O. and Ha, Y. L. 2004. Inhibition of free radicalinduced lipid oxidation by the extract from submerged-liquid culture of mushrooms in the medium containing mulberry tree powders. J. Kor. Soc. Food Sci. Nutr. 33(2):255-261. https://doi.org/10.3746/jkfn.2004.33.2.255
  14. Kobayashi, T., Vieira, W. D., Potterf, B., Sakai, C. and Imokawa, G. 1995. Modulation of melanogenic protein expression during the switch from eu-to phaeomelanogenesis. J. Cell Sci. 108:2301-2309.
  15. Kobayashi, T., Imokawa, G., Bennett, D. C. and Hearing, V. J. 1998. Tyrosinase stabilization by Tyrpl(the brown locus protein). J. Biol. Chem. 273(48):3180-3185. https://doi.org/10.1074/jbc.273.6.3180
  16. Koh, J. B. and Choi M. A., 2001, Effect of Cordyceps militraris on lipid metabolism in rats fed cholesterol diet. Kor. Nutri. Soc. 34(3):265-270.
  17. Koh, J. B. 2002. The Effects of Cordyceps militaris on Lipid Metabolism, protein levels and enzyme activities in rats fed a high fat diet. Kor. Nutri. Soc. 35(4):414-420.
  18. Kwon, S. H., Woo, H. J., Han. D. S. and Kim, M. K. 2001. Effect of dried powders and water extracts of paecilomyces tenuipes and cordyceps militaris on lipid metabolism, antioxidative capacity and immune status in rats. Kor. Nutri. Soc. 34(3):271-284.
  19. Lee, E. H., Lee, J. K., Hong, J. T., Jung, K. M., Kim, Y. K., Lee, S. E., Chung, S. Y. and Lee, Y. W. 2001. Protective effect green tea extract catechin on UVB induced skin damage. J. Food Hygiene and Safety 16(2):117-124.
  20. Lee, H. M., Lee, Y. J. and Park, T. S. 2004. Tumor growth inhibitory and immunomodulatory activities of Cordyceps militaris water extracts in icr mice bearing sarcoma-180 solid tumor. J. Kor. Soc. Food Sci. Nutr. 33(1):59-65. https://doi.org/10.3746/jkfn.2004.33.1.059
  21. Lee, J. D. 1998. Mycology 3rd edition, Gu-duck publishing company.
  22. Lee, S. H., Ko, S. S., Youn, Y. J. and Lee, J. I. 2002. Effect of Cordyceps militaris on maximal aerobic power and recovery of fatigue. JKSSPE 6(2):187-193.
  23. Lim, H. W., Kwon, Y. M., Cho, S. M., Kim, J. H., Yoon G. H., Lee, S. J., Kim H. W. and Lee, M. W. 2004. Antitumor activity of Cordyceps militaris on human cancer cell line. Kor. J. Pharmacogn 35(4):364-367.
  24. Mason, H. S. 1948. The chemistry of melanin. III. Mechanism of the oxidation of trihydroxyphenylalanine by tyrosinase. J. Biol. Chem. 172:83-99.
  25. Maeda, K. and Fukuda, M. 1991. In vitro effectiveness of several whitening cosmetic components in human melanocytes. J. Soc. Cosmetic Chemists 42(6):361-368.
  26. Oh, S. W., Kim, S. H., Song, H, N. and Han, D. S. 2003. Comparative Chemical Composition of Four Kinds of Tochukaso. Kor. J. Food Sci. Technol, 35(1):15-22.
  27. Olivares, C., Jimenez-Cervantes, C., Lozano, J. A., Solano, F. and Garcia- Borron, J. C. 2001. The 5,6-dihydroxyindole-2-carboxylic acid (DHICA) oxidase activity of human tyrosinase. Biochem. J. 354: 131-139. https://doi.org/10.1042/0264-6021:3540131
  28. Park, C. S., Kwon, C. J., Choi, M. A., Park, G. S. and Choi, K. H. 2002. Antibacterial activities of Cordyceps spp., Mugwort and Pine needle Extracts. Kor. J. Food Preservation 9(1):109-113.
  29. Park, S. N. 1997. Skin aging and oxygen species. J. Cosmetic Sci. 23(1):75-132.
  30. Parvez, S., Kang, M. K., Chung, H. S., Cho, C. W., Hong, M. C., Shin, M. K. and Bae, H. S. 2006. Survey and mechanism of skin depigmenting and lightening agents. Phytother Res. 10:1002
  31. Raper, H. S. 1928. The anaerobic oxidases. Physiol. Rev. 8:245-282.
  32. Sugimoto, K., Nishhura, T., Nomura, K. and Kuriki, T. 2004. Inhibitory effects of alpha arbutin on melanin synthesis in cultured human melanoma cells and a three-dimensional human skin model. Biol & Pharmaceutical Bulletin 27(4):510-514. https://doi.org/10.1248/bpb.27.510
  33. Terasaka, H., Takayama, F., Satoh, K., Fujisawa, S. and Sakagami, H. 2000. Effect of antioxidants on radical intensity and cytotoxicity of hydroquinone. Anticancer Res. 20(5):3357-3362.

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