Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Association of Microsatellite Marker in FABP4 Gene with Marbling Score and Live Weight in Hanwoo

  • Lee, Seung-Hwan (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R.D.A.) ;
  • Cho, Yong-Min (Green Growth & Future Strategy Team, R.D.A.) ;
  • Kim, Hyeong-Cheol (Hanwoo Experiment Station, National Institute of Animal Science, R.D.A.) ;
  • Lim, Da-Jeong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R.D.A.) ;
  • Moon, Hee-Joo (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R.D.A.) ;
  • Hong, Seong-Koo (Hanwoo Experiment Station, National Institute of Animal Science, R.D.A.) ;
  • Oh, Sung-Jong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R.D.A.) ;
  • Kim, Tae-Hun (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R.D.A.) ;
  • Yoon, Du-Hak (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R.D.A.) ;
  • Park, Eung-Woo (Cooperation & Governance Unit Research Policy Planning Division, Research Policy Bureau, R.D.A.)
  • Received : 2010.10.12
  • Accepted : 2010.12.14
  • Published : 2010.12.31
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Abstract

The bovine fatty acid binding protein 4 (FABP4) plays an important role to uptake intracellular fatty acid. It has been previously reported as a positional candidate gene for marbling score in livestock. The re-sequencing of FABP4 gene detected a polymorphic AT repeated sequence in intron II of FABP4 gene. Allelic distribution for this microsatellite marker was examined in other cattle breeds. A total of 8 alleles were detected with diverse repeat units (14 to 21 AT repeat) in Hanwoo and 7 breeds. Of the 8 alleles, the predominant alleles were $[AT]_{16}$, $[AT]_{18}$ and $[AT]_{19}$ in the Hanwoo and 7 cattle breeds. The linear mixed model for genotypic effect (3237AT) on carcass traits showed a significant effect on marbling score (MAR P=0.025) and live weight (LWT; P=0.04) in the 583 Hanwoo cattle population. Live weight (LW) was highest in the homozygous $(AT)_{17}$ genotype ($557.5{\pm}6.94$) and lowest in the heterozygous $(AT)_{16/17}$ genotype ($521.7{\pm}7.70$). On the other hand, the homozygous $(AT)_{17}$ genotype ($3.0{\pm}0.15$) has the highest effect on marbling score and the lowest effect was in homozygous (AT)$_{18}$ genotype ($2.2{\pm}0.15$). The marbling score difference between both groups was 0.8 which is around two times higher than SNP genotype effect on marbling score in Limousin $\times$ Wagyu crosses.

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References

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