Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Production of All-Female using Sex-Reversal Gynogenetic Diploid in Rainbow Trout, Oncorhynchus mykiss

무지개송어의 자성발생2배체 성전환 수컷을 이용한 전 암컷 생산

  • Lee, Cheul-Ho (Cold-Water Fish Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Dae-Jung (Inland Aquaculture Research Center, NFRDI) ;
  • Jeong, Chang-Hwa (Binex, Inc.) ;
  • Choi, Gyeong-Cheol (Chungcheongbuk-do Inland Fisheries Research Institute) ;
  • Lee, Chae-Sung (Cold-Water Fish Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Dong-Soo (Department of Aquaculture, Pukyong National University)
  • Received : 2010.09.06
  • Accepted : 2010.10.07
  • Published : 2010.12.30
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Abstract

This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. Induction of sex-reversed gynogenetic diploid rainbow trout males and mass production of all-female rainbow trout by genetic sex reversal was performed. Phenotypic males in the gynogenetic diploid group were induced successfully by dietary administration of 5 mg of 17 alpha-methyltestosterone per kg diet for 82 days. All females were produced by crossing between normal female and sex-reversed gynogenetic diploid male rainbow trout.

본 연구는 무지개송어 양식 산업의 생산성 향상을 위한 일환으로 염색체공학 기법을 이용하여 전 암컷 무지개 송어의 대량생산을 유도 하였다. 자성발생 2배체를 유도한 후 17 alpha-methyltestosteron으로 성전환을 성공적으로 유도하였다. 성전환된 수컷에서는 일반 암컷 모양을 띤 생식소가 형성 되었으나, 수정관의 발달은 보이지 않는 전형적인 성전환 개체의 특징을 나타내었다. 성전환된 자성발생 2배체 가짜수컷을 이용하여 정상 암컷과 단순교배로 발안율 55.7%, 부화율 52.9%의 전 암컷 집단 97,850 마리를 얻을 수 있었고, 4개월 사육 후 치어기(6~7 cm)때의 생식소 확인 결과 100% 암컷임이 확인되었다.

Keywords

References

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