Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Verification of the Fractions with Strong Estrogenic Activities from Brown Algae

갈조류로부터 에스트로겐 고활성 분획의 검증

  • Lee, Seung-Woo (Department of Pharmaceutical Engineering, Silla University) ;
  • Jang, Min-Kyung (Department of Pharmaceutical Engineering, Silla University) ;
  • Kim, Nam-Young (Department of Pharmaceutical Engineering, Silla University) ;
  • Jang, Hye-Ji (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, Silla University) ;
  • Kim, Mi-Hyang (Department of Food and Nutrition, Silla University) ;
  • Kim, Yuck-Young (Bioport Korea Co., Marine Bio-industry Department Center) ;
  • Kim, Sung-Gu (IS Food Co., Marine Bio-industry Department Center) ;
  • Yoo, Byung-Hong (IS Food Co., Marine Bio-industry Department Center) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University)
  • 이승우 (신라대학교 의생명과학대학 제약공학과) ;
  • 장민경 (신라대학교 의생명과학대학 제약공학과) ;
  • 김남영 (신라대학교 의생명과학대학 제약공학과) ;
  • 장혜지 (신라대학교 의생명과학대학 제약공학과) ;
  • 이동근 (신라대학교 의생명과학대학 제약공학과) ;
  • 김미향 (신라대학교 의생명과학대학 식품영양학과) ;
  • 김육용 ((주) 아이에스푸드) ;
  • 김성구 ((주) 바이오포트 코리아) ;
  • 유병홍 ((주) 바이오포트 코리아) ;
  • 이상현 (신라대학교 의생명과학대학 제약공학과)
  • Received : 2010.08.06
  • Accepted : 2010.11.18
  • Published : 2010.12.30
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Abstract

In order to evaluate estrogenic compounds in brown algae, an in vitro test system for the verification of estrogenic activity was applied. Fractions from ethanol extracts of each brown alga were prepared by a systematic fractionation procedure with solvents such as $H_2O$, hexane, butanol and methanol. Aqueous fractions of brown algae showed the highest estrogenic activities. Estrogenic activities of $500\;{\mu}g/ml$ aqueous fractions of Undaria pinnatifida and Laminaria japonica showed almost the same strength as that of $10^{-7}\;M$ standard solution ($17{\beta}$-estradiol). Furthermore, estrogenic activities of $500\;{\mu}g/ml$ aqueous fractions of Ecklonia stolonifera and Porphyra suborbiculate represented higher activities than that of $10^{-8}\;M$ $17{\beta}$-estradiol. These observations suggest that aqueous fractions of all these brown algae are expected to possess estrogenic compounds and could be developed as estrogenic agents for postmenopausal disorder.

갈조류에서 에스트로겐 활성소재를 평가하기 위하여 in vitro 검출시스템을 사용하여 에스트로겐 효과를 가지는 분획을 검증하였다. 각각의 갈조류의 분획물들은 에탄올 추출로부터 물, 헥산, 부탄올 그리고 메탄올의 용매를 이용한 분획과정을 통해 제조하였다. 갈조류들의 수층 분획물에서 가장 높은 에스트로겐 활성을 보였다. 미역과 다시마의 수층 분획물들은 $500\;{\mu}g/ml$의 농도에서 $10^{-7}\;M$의 표준물질($17{\beta}$-estradiol)과 비슷한 활성을 보였으며, 곰피와 돌자반의 수층 분획물들은 $500\;{\mu}g/ml$의 농도에서 $10^{-8}\;M$의 표준물질보다 높은 활성을 보였다. 이 연구의 결과로 갈조류인 미역, 다시마, 곰피 및 돌자반의 수층 분획물에 에스트로겐 대체 작용을 할 수 있는 성분이 포함되어 있는 것을 확인하였으며, 이를 이용하여 폐경기 장애를 예방할 수 있는 에스트로겐 활성소재의 개발이 가능할 것으로 기대된다.

Keywords

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