Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Buan Mulberry on Metabolic Improvement in Streptozotocin-Induced Diabetic Rats

Streptozotocin으로 유발된 당뇨쥐의 신진대사 증진에 대한 부안 오디의 효과

  • Lee, Ju-Taek (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Ryu, Jae-Sung (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Kwak, Dong-Hoon (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Park, Yun-Jum (Division of Horticulture and Pet Animal-Plant Science, Wonkwang University) ;
  • Kang, Seong-Sun (Buan Agricultural Development & Technology Center) ;
  • Kim, Pyoung-Jun (Buan Agricultural Development & Technology Center) ;
  • Hwang, Kyung-A (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Choo, Young-Kug (Department of Biological Science, College of Natural Sciences, Wonkwang University)
  • 이주택 (원광대학교 자연과학대학 생명과학부) ;
  • 류재성 (원광대학교 자연과학대학 생명과학부) ;
  • 곽동훈 (원광대학교 자연과학대학 생명과학부) ;
  • 박윤점 (원광대학교 생명자원과학대학 원예.애완동식물학부) ;
  • 강성선 (전라북도 부안군 농업기술센터) ;
  • 김병준 (전라북도 부안군 농업기술센터) ;
  • 황경아 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 추영국 (원광대학교 자연과학대학 생명과학부)
  • Received : 2010.08.31
  • Accepted : 2010.11.17
  • Published : 2010.12.30
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Abstract

This study was designed to evaluate the anti-diabetic effects of Buan mulberries by using an insulin-dependent diabetes mellitus animal model. Several studies have shown that mulberries have metabolism-improving, antioxidant, and lipid-lowering properties in rats with streptozotocin (STZ)-induced diabetes. In this study, Sprague-Dawley male rats were randomly assigned to 1 normal control group and 5 STZ-induced diabetes groups: rats that had STZ-induced diabetes and did not receive any agents (diabetic group; negative control), rats that had STZ-induced diabetes and received insulin (insulin group; positive control), rats that had STZ-induced diabetes and received 0.5% mulberry extract (0.5% mulberry group), rats that had STZ-induced diabetes and received 1.0% mulberry extract (1.0% mulberry group), and rats that had STZ-induced diabetes and received 2.0% mulberry extract (2.0% mulberry group). Mulberry extracts were administered to the diabetic animals for 4 weeks. The rats that received mulberry extracts showed lower body weights and insulin levels, as well as higher kidney weights, blood glucose levels, urine quantities, and water intake in comparison with the normal controls. Further, the insulin concentrations in the mulberry-fed animals were higher than those in the diabetic group, and the kidney weights, blood glucose levels, urine quantities, and water intake in the mulberry-fed animals were lower than the corresponding values in the diabetic controls. These results suggest that mulberry may be an effective functional food to prevent diabetes-related complications.

본 연구에서는 streptozotocin (STZ)에 의해 유도된 당뇨병 흰쥐를 이용하여 부안산 오디의 항 당뇨효과를 in vivo 실험을 중심으로 조사하였다. 기존의 연구는 주로 in vitro 실험을 통해 이루어 졌으며 일부 지역의 오디는 STZ에 의해 유도된 당뇨병 흰쥐에서 신진대사 증진, 항산화 및 체내 지방 저하 효과가 있음이 시사 되어졌다. 먼저, Sprague-Dawley 수컷 흰쥐를 난괴법을 통해 하나의 정상군(Normal)과 대조군(Diabetic), 인슐린 처리군(Insulin), 0.5% 오디 투여군(0.5% Mulberry), 1.0% 오디 투여군(1.0% Mulberry) 그리고 2.0% 오디 투여군(2.0% Mulberry) 등으로 분류 한 후 STZ로 당뇨병을 유도하였다. 부안산 오디를 STZ에 의한 당뇨병 흰쥐에 4주간 투여한 후 정상군과 비교 했을때 다양한 농도의 오디를 투여한 그룹에서 몸무게, 혈중 인슐린의 농도는 감소했지만, 신장무게, 혈당량, 요량 및 음수량은 증가하였다. 추가적으로 STZ에 의한 당뇨병 흰쥐와 이들 동물들에게 오디를 투여한 그룹을 비교한 결과 오디를 투여한 그룹은 신장 무게, 혈당, 요량 및 음수량이 확연히 감소하였다. 또한 오디를 투여한 그룹에서의 인슐린 농도는 대조군과 비교하여 유의적으로 증가하였다. 이와 같은 연구결과로부터 부안산 오디는 향후 당뇨 합병증을 억제하기 위한 기능성 식품의 원료로 사용 되어질 가능성을 시사한다.

Keywords

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