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Genotypic Characterization of Oak Wilt Pathogen Raffaelea quercus-mongolicae and R. quercivora Strains

참나무류에 시들음병을 일으키는 Raffaelea quercus-mongolicae와 R. quercivora의 유전적 특성

  • Received : 2010.09.03
  • Accepted : 2010.10.14
  • Published : 2010.12.01

Abstract

Recently, the oak wilt diseases especially on Quercus mongolica, have been increasing in various districts of Korea. A collection of 38 strains of the oak wilt pathogen Raffaelea qeurcus-mongolicae and R. quecivora isolated from Quercus spp. in Korea and Japan was characterized by $\beta$-tubulin gene sequence and randomly amplified polymorphic DNA (RAPD) analysis. In cluster analysis based on $\beta$-tubulin gene sequence the strains were divided into 4 clusters, of which clusters 2 and 4 were composed of Japanese strains except for one Korean strain. RAPD analysis showed that they were also effectively differentiated by a strong RAPD fragments. On the basis of the two genetic analysis, significant differences were detected between Korean strains and Japanese strains.

최근 신갈나무를 중심으로한 참나무과 나무에 시들음병 피해가 증가하고 있다. 한국과 일본에서 분리한 38개의 시들음병 균주(Raffaelea quercus-mongolicae, R. quercivora)에 대해 $\beta$-tubulin 유전자 염기서열 분석과 RAPD 분석을 이용해 유전적 특성을 조사하였다. $\beta$-tubulin 유전자 염기서열을 이용한 cluster 분석결과 시들음병 균주들은 4개의 그룹으로 나뉘었으며, cluster 2와 4에는 1균주를 제외하고 모두 일본균주가 속해 있었다. RAPD 분석결과 시들음병 균주들은 3개의 그룹으로 나뉘었으며, 한국균주와 일본균주는 쉽게 구별되었다. $\beta$-tubulin 유전자 염기서열 분석과 RAPD 분석결과 한국균주와 일본균주는 상당히 다른 유전적 특성을 가지고 있었다.

Keywords

References

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Cited by

  1. Assessment of Virulence of Raffaelea quercus-mongolicae and Raffaelea spp. Isolates by Artificial Inoculation of Quercus mongolica Logs vol.20, pp.4, 2014, https://doi.org/10.5423/RPD.2014.20.4.270