KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimization of Lipid Extraction from Scenedesmus sp. Using Taguchi Approach

Scenedesmus sp.로부터 Taguchi 법을 이용한 지방추출의 최적화

  • Kim, Na-Young (Department of Bioengineering and Technology, Kangwon National University) ;
  • Oh, Sung-Ho (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Choi, Woon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
  • 김나영 (강원대학교 생물공학과) ;
  • 오성호 (강원대학교 바이오산업공학부) ;
  • 최운용 (강원대학교 바이오산업공학부) ;
  • 이현용 (강원대학교 바이오산업공학부) ;
  • 이신영 (강원대학교 생물공학과)
  • Received : 2010.01.26
  • Accepted : 2010.08.25
  • Published : 2010.08.31
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Abstract

For the biodiesel fuel production from microalgae, the lipid from wet and dry samples of green algae Scenedesmus sp. was extracted by using various solvents and pre-treatment methods. Extraction yield of the lyophilized sample was better than that of dry sample. Chloroform/methanol (2:1, v/v) and ultrasonication or homogenization method were also selected as the most effective solvent and pre-treatment methods for lipid extraction, respectively. Under these constraint conditions, optimization experiment of lipid extraction was investigated by Taguchi approach using orthogonal matrix $L_9$ ($3^4$) method. The optimum extraction conditions of lipid extraction was obtained at pre-treatment of homogenization, extraction time of 5 hour, temperature of $35^{\circ}C$, and solvent ratio of 1:20 (w/v). Yield of extraction at optimized condition was 20.55% and it was 96% of total lipid content (21.38%) of Scenedesmus sp.

Keywords

References

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