KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Extraction, Purification and Property of the lipid from Scenedesmus sp.

Scenedesmus sp.로 부터의 지방 추출, 정제 및 특성

  • Kim, Na-Young (Department of Bioengineering and Technology, Kangwon National University) ;
  • Oh, Sung-Ho (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Choi, Woon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
  • 김나영 (강원대학교 생물공학과) ;
  • 오성호 (강원대학교 바이오산업산업공학부) ;
  • 최운용 (강원대학교 바이오산업산업공학부) ;
  • 이현용 (강원대학교 바이오산업산업공학부) ;
  • 이신영 (강원대학교 생물공학과)
  • Received : 2010.01.19
  • Accepted : 2010.08.25
  • Published : 2010.08.31
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Abstract

Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was $21.38{\pm}1.03$ wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of $C_{16}{\sim}C_{22}$ with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.

Keywords

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