Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Comparison of Cuticular Hydrocarbons of the Pine Sawyer (Monochamus saltuarius), Japanese Pine Sawyer (Monochamus alternatus) and Oak Longicorn Beetle (Moechotypa diphysis)

북방수염하늘소(Monochamus saltuarius), 솔수염하늘소(Monochamus alternatus), 털두꺼비하늘소(Moechotypa diphysis) 성충의 표피탄화수소 비교

  • Lee, Jeong-Eun (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Kim, Eun-Hee (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University)
  • 이정은 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김은희 (충북대학교 농업생명환경대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2010.07.20
  • Accepted : 2010.07.09
  • Published : 2010.09.30
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Abstract

Cuticular hydrocarbons (CHCs) of the pine sawyer (Monochamus saltuarius), Japanese pine sawyer (M. alternatus) and oak longicorn beetle (Moechotypa diphysis) were analyzed by GC, GC-MS and compared. Monochamus beetles are typical vectors of pine wilt disease but Moechotypa diphysis, which belongs to the same family, is not. They possess different CHCs in carbon number: 23-25 in M. saltuarius, 25-32 in M. alternatus, and 23-29 in M. diphysis. In comparison to inter-species, these three species of adult beetles have different numbers and chains of constituents of CHCs. In comparison between male and female in intra-species, the quantities of CHCs show the difference but constituents are not. Major constituent of M. saltuarius were analyzed as n-pentacosane > n-nonacosane > n-heptacosane; those of M. alternatus were n-nonacosene > n-pentacosane > n-nonacosane; and those of M. diphysis were n-heptacosane > 13-methylheptacosane > 3-methylheptacosane. From the body surface, most saturated carbohydrates of 3 species beetles are composed of n-alkane (40.2 - 65.7%) and followed by olefines > monomethylalkanes that one or two double bonds in M. saltuarius and M. alternatus. Otherwise, M. diphysis have the difference in order of monomethylalkanes > olefins.

북방수염하늘소, 솔수염하늘소, 털두꺼비하늘소 성충 암·수의 표피탄화수소를 GC와 GC-MS로 분석하고 비교하였다. 북방수염하늘소와 솔수염하늘소는 소나무재선충병의 대표적 매개충이지만 같은 과에 속한 털두꺼비하늘소는 그렇지 않다. 이들은 표피탄화수소 사슬을 다르게 가지고 있는데, 북방수염하늘소의 표피탄화수소 사슬은 $C_{23-35}$, 솔수염하늘소는 $C_{25-32}$, 그리고 털두꺼비하늘소는 $C_{23-29}$로 종간 차이를 보였다. 주 구성성분의 암 수간 차이에서 함량에 대한 차이는 있었지만, 조성에 대한 차이는 없었다. 북방수염하늘소는 n-pentacosane > n-nonacosane > n-heptacosane, 솔수염하늘소는 n-nonacosene > n-pentacosane > n-nonacosane을 가장 많이 포함하고 있었으며, 털두꺼비하늘소는 n-heptacosane > 13-methylheptacosane > 3-methylheptacosane을 가장 많이 포함하고 있는데 반해 n-nonacosene, n-pentacosane의 함량은 적었다. 3종하늘소 표피의 대부분은 n-alkane인 포화탄화수소로 이루어져 있고(40.2 - 65.7%) 그 다음으로, 북방수염하늘소와 솔수염하늘소는 하나 또는 두 개의 이중결합을 갖는 olefins > monomethylalkanes 순이었다. 이와 달리, 털두꺼비하늘소는 monomethylalkanes > olefins 순으로 함량에 차이가 있었다.

Keywords

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