The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Lipid Modulatory Functions of Cysteine Compounds Found in Genus Allium Plants in Diabetic Mice

Allium 속 식물 Cysteine화합물이 당뇨 쥐의 지질대사에 미치는 영향

  • Received : 2010.06.28
  • Accepted : 2010.07.19
  • Published : 2010.09.30
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Abstract

In this study, cysteine compounds found in genus Allium plants, including N-acetyl cysteine(NAC), S-allyl cysteine(SAC), S-ethyl cysteine(SEC), and S-methyl cysteine(SMC), were examined for effects on blood glucose, glucose tolerance, and plasma lipid concentrations in streptozotocin(STZ)-induced diabetic mice. In the mice, the ingestion of these cysteine compounds did not affect blood glucose levels significantly. However, their ingestion did improve the diabetic symptoms of polydipsia, polyphagia, polyuria, and weight loss. Glucose tolerance was also found to be improved in the STZ diabetic animals by feeding the cysteine compounds. Treatment of the compounds also caused a slight decrease in plasma concentrations of total cholesterol along with increases in HDL-cholesterol and slight decreases in LDL-cholesterol, resulting in a significant decrease in the atherogenic index of plasma in the diabetic animals. They also showed reductions of liver triglyceride content to relieve diabetic fatty liver syndrome. In summary, the cysteine compounds such as NAC, SAC, SEC, and SMC, found in genus Allium plants, had certain beneficial effects on blood glucose metabolism along with preventing abnormalities in lipid metabolism, a complication of diabetes, by improving the atherogenic index of plasma and fatty liver in STZ-induced diabetic mice.

Streptozotocin(STZ)으로 유도된 당뇨 쥐에서 마늘, 양파 등 Allium 속(屬) 식물에 함유되어 있는 N-acetyl cysteine(NAC), S-allyl cysteine(SAC), S-ethyl cysteine(SEC) 및 S-methyl cysteine (SMC) 등 organocysteine 화합물이 혈당과 포도당 내성 및 혈장과 간 조직의 지질 농도에 미치는 영향을 살펴보았다. NAC, SAC, SEC 및 SMC 등 cysteine 화합물 투여는 STZ 당뇨로 인한 다음, 다식, 다뇨 및 체중 감소 증세를 일부 개선시키고, 내당능을 향상시키는 결과를 보였다. 당뇨 쥐에 있어서 cysteine 화합물의 투여는 혈장 총 콜레스테롤과 중성지질 농도에는 큰 영향을 미치지 않았으나, HDL-cholesterol의 농도의 증가와 LDL-cholesterol 농도의 감소를 통해 전반적으로 동맥경화 유발지수를 개선시키는 결과를 보였으며, 간 조직 중성지질 농도를 낮추어 당뇨군의 지방간 증세를 완화시킴과 아울러 혈장 GOT, GPT 활성을 감소시켜 간 보호 효과를 보였다. 이들 결과를 볼 때 NAC, SAC, SEC 및 SMC 등 cysteine 화합물은 STZ에 의해 유도된 당뇨 쥐에 있어 당대사 조절과 함께 지질대사 조절을 통해 지질대사 이상으로 인한 합병증 유발을 완화시킬 수 있는 가능성이 있다고 사료된다.

Keywords

References

  1. Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC. 1974. Enzymatic determination of total serum cholesterol. Clin Chem 20:470-475
  2. Anderson JW. 1990. Dietary fiber and nutrition management of diabetes mellitus In: Chen SC, ed. Proceedings of Kellog International Symposium on Dietary Fiber. pp.59-68. Center for Academic Publications, Japan
  3. Burstein M, Scholnick HR, Morfin R. 1970. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res 11:583-595
  4. Coulston AM, Hollenbeck CB. 1988. Source and amount of dietary carbohydrate in patients with noninsulin-dependent diabetes mellitus. Top Clin Nutr 3:17-24
  5. Dobiasova M, Frohlich J. 2001. The plasma parameter log(TG/ HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate in apoB-lipoprotein-depleted plasma(FERHDL). Clin Biochem 34:583-588 https://doi.org/10.1016/S0009-9120(01)00263-6
  6. Folch J, Lees M, Stanley GHS. 1957. A simplified method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497-509
  7. Franois B. 1996. Non-insulin dependent diabetes and reverse cholesterol transport. Atherosclerosis 124(suppl):S39-S42
  8. Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499-502
  9. Goldberg RB. 1981. Lipid disorders in diabetes. Diabetes Care 4:561-572 https://doi.org/10.2337/diacare.4.5.561
  10. Han HJ, Kim HJ, Chong MS, Cho HE, Choi YH, Lee KN. 2009. Effect of red ginseng with processed sulfur extracts on serum lipids concentration and metabolic variables in diabetic rats. Kor J Herbology 24:89-98
  11. Health Insurance Review & Assessment Service. 2009. 2008 National Health Insurance Statistical Yearbook. pp.6, pp.544-550. National Health Insurance Corporation
  12. Hsu CC, Huang CN, Hung YC, Yin MC. 2004. Five cysteinecontaining compounds have amtioxidative activity in Balb/cA mice. J Nutr 134:149-152
  13. Kahn CR. 1985. The molecular mechanism of insulin action. Ann Rev Med 36:249-251
  14. Kim HS. 2004. Effects of the Prunus persica Batsch var. davidiana Max. extract on the blood glucose and serum lipid components in streptozotocin-induced diabetic rats. Korean J Food & Nutr 17:337-345
  15. Lalla E, Lamster IB, Drury S, Fu C, Schmidt AM. 2000. Hyperglycemia, glycoxidation and receptor for advanced glycation endproducts: potential mechanisms underlying diabetic complications, including diabetes-associated periodontitis. Periodontology 23:50-62 https://doi.org/10.1034/j.1600-0757.2000.2230104.x
  16. Lin CC, Yin MC, Hsu CC, Lin MP. 2004. Effect of five cysteinecontaining compounds on three lipogenic enzymes in Balb/ cA mice consuming a high saturated fat diet. Lipids 39: 843-848 https://doi.org/10.1007/s11745-004-1305-4
  17. Lin CC, Yin MC, Liu WH. 2008. Alleviative effects of s-allyl cysteine and s-ethyl cysteine on MCD diet-induced hepatotoxicity in mice. Food Chem Toxicol 46:3401-3406 https://doi.org/10.1016/j.fct.2008.08.010
  18. Liu L, Yeh YY. 2001. Water-soluble organosulfur compounds of garlic inhibit fatty acid and triglyceride syntheses in cultured rat hepatocytes. Lipids 36:395-400 https://doi.org/10.1007/s11745-001-0734-4
  19. Matkovics B, Kotorman M, Varga IS, Hai DQ, Varga C. 1998. Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung 85:29-38
  20. Min HK, Yoo HJ, Lee HK, Kim EJ. 1981. Changing patterns of the prevalence of diabetes mellitus in Korea. J Kor Diabetes Assoc 6:1-4
  21. Morel DW, Chisolm GM. 1989. Antioxidative treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity. J Lipid Res 30:1827-1834
  22. Park JS, Yang JS, Hwang BY, Yoo BK, Han K. 2009a. Effect of yacon tuber extract and its constituent, chlorogenic acid, in streptozotocin-induced diabetic rats. Biomol Ther 17:256-262 https://doi.org/10.4062/biomolther.2009.17.3.256
  23. Park YK, Kim JS, Jeon EJ, Kang MH. 2009b. The improvement of Chaga mushroom(Inonotus obliquus) extract supplementation on the blood glucose and cellular DNA damage in streptozotocininduced diabetic rats. Korean J Nutr 42:5-13
  24. Preston AM, Tome J, Morales JJ, Milan L, Cuevas AA, Medina J, Santiago JA. 1991. Diabetic parameters 58 weeks after injection with streptozotocin in rats fed basal diets or diets supplemented with fiber, mineral and vitamins. Nutr Res 11:895-906 https://doi.org/10.1016/S0271-5317(05)80617-7
  25. Reaven KM. 1987. Abnormal lipoprotein metabolism in noninsulin dependent diabetes mellitus. Am J Med 83:31-40
  26. Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:58-63
  27. Sheng J, Yuji M, Kenji M, Shinji K, Tadash, N, Katsuto T, Masharu K, Seiichiro T. 1988. Increased activity of intestinal Acyl-Co A: Cholesterol acyltransferase in rats with streptozotocininduced diabetes and restoration by insulin supplement. Diabetes 37:342-346 https://doi.org/10.2337/diabetes.37.3.342
  28. Takada N, Yano Y, Wanibuchi H, Otani S, Fukushima S. 1997. S-methylcysteine and cysteine are inhibitors of induction of glutathione S-transferase placental form-positive foci during initiation and promotion phases of rat hepatocarcinogenesis. Jpn J Cancer Res 88:435-442 https://doi.org/10.1111/j.1349-7006.1997.tb00401.x
  29. Vijayaraghavan M, Wanibuchi H, Takada N, Yano Y, Otani S, Yamamoto S, Fukushima S. 2000. Inhibitory effects of S-methylcysteine and cysteine on the promoting potential of sodium phenobarbital on rat liver carcinogenesis. Jpn J Cancer Res 91:780-785 https://doi.org/10.1111/j.1349-7006.2000.tb01013.x
  30. Welch C, Wuarin L, Sidell N. 1992. Antiproliferative effect of the garlic compound S-allyl cysteine on human neuroblastoma cells in vitro. Cancer Lett 63:211-219 https://doi.org/10.1016/0304-3835(92)90263-U
  31. West KM, Ahuja MS, Bennett PH. 1983. The role of circulating glucose and triglyceride concentrations and their interaction with other "risk factors" as determinants of arterial disease in nine diabetic population samples from the WHO multinational study. Diabetes Care 6:361-369 https://doi.org/10.2337/diacare.6.4.361
  32. Yoon JA, Son YS. 2009. Effects of Opuntia ficus-indica complexes B(OCB) on blood glucose and lipid metabolism in streptozotocininduced diabetic rats. Korean J Food & Nutr 22:48-56