The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effects of Paprika (Capsicum annuum L.) on Serum Lipid Profile and $\alpha$-Tocopherol Concentration in Rats Fed a High-Cholesterol Diet

파프리카 급여가 고콜레스테롤 식이 흰쥐의 혈청 지질과 $\alpha$-Tocopherol 농도 변화에 미치는 영향

  • Park, Jae-Hee (Dept. of Food and Nutrition, Kyungnam University) ;
  • Noh, Sang-K. (Dept. of Food and Nutrition, Changwon National University) ;
  • Kim, Chang-Soon (Dept. of Food and Nutrition, Changwon National University)
  • 박재희 (경남대학교 식품영양학과) ;
  • 노상규 (창원대학교 식품영양학과) ;
  • 김창순 (창원대학교 식품영양학과)
  • Received : 2010.05.28
  • Accepted : 2010.06.30
  • Published : 2010.09.30
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Abstract

In conclusion, rats were fed diets containing either NC, HC, HC-FDP, HC-WEP, or HC-EEP for 6 weeks. At the 6th week, increases in body weight and visceral fat were lower in the paprika fed groups as compared to the HC group. Serum levels of triglycerides, total cholesterol, low density lipoprotein cholesterol, and atherogenic index values were significantly lower in the paprika diet fed groups than the HC group (p<0.05). In particular, the lipid lowering effects in the HC-EEP group were superior among the paprika fed groups. Also, serum $\alpha$-tocopherol levels were lower in the control group compared to the paprika fed groups. The supplementation of paprika may exert lipid lowering effects and saving effect of $\alpha$-tocopherol in the serum of high-cholesterol diet fed rats. However, it should be noted that the results are based on very small sample numbers and a short experimental period.

본 연구는 추출물 형태에 따른 파프리카 첨가 식이가 고콜레스테롤 식이 흰쥐의 혈청 지질과 $\alpha$-tocopherol 농도에 미치는 영향을 알아보기 위해 Sprague-Dawley 수컷 25마리를 대조군(NC), 고콜레스테롤군(HC), 고콜레스테롤+동결건조 파프리카 분말 섭취군(HC-FDP), 고콜레스테롤+파프리카 물 추출물 섭취군(HC-WEP), 고콜레스테롤+파프리카 에탄올 추출물 섭취군(HC-EEP)으로 나누어 실험 식이를 6주간 공급하였다. 6주 후 HC군과 파프리카 급여군에서 체중 증가는 각각 85.2%와 65.5~68.88%로 HC군에서 체중이 유의적으로 증가하였다. 그리고 내장지방 무게는 HC군보다 파프리카 급여군에서 감소하였으며, 파프리카 급여군의 중성지질, 총 콜레스테롤, LDL-콜레스테롤과 atherogenic index 역시 HC군보다 유의적으로 감소하였다(p<0.05). 그리고 파프리카 급여군들 중에서는 HC-EEP 군이 혈청지질농도 감소 효과가 가장 큰 것으로 나타났다. 혈청 $\alpha$-tocopherol 농도는 HC-EEP에서 $30.5{\pm}0.6\;{\mu}mol/{\ell}$로 가장 높았고, 그 다음으로는 HC-WEP($30.0{\pm}0.8\;{\mu}mol/{\ell}$)>HC-FDP($29.1{\pm}0.5\;{\mu}mol/{\ell}$)>HC($27.7{\pm}0.3\;{\mu}mol/{\ell}$)이었다. 이는 고콜레스테롤 식이에 의한 산화적 스트레스를 감소시키기 위하여 혈청 $\alpha$-tocopherol이 소모되는 것을 파프리카 급여로 절약하는 효과를 나타낸 것으로 사료된다. 따라서 본 연구를 통해 파프리카는 혈중 지질 농도 저하 효과를 보였고, $\alpha$-tocopherol 절약 작용을 통해 체내 항산화제로서 활용 가능성을 확인할 수 있었다.

Keywords

References

  1. Aizawa T, Inakuma T. 2009. Dietary capsanthin, the main carotenoid in paprika (Capsicum annuum L.), alters plasma highdensity lipoprotein cholesterol levels and hepatic gene expression in rats. Br J Nutr 31:1-7
  2. Allard JP, Royall D, Kurian R, Muggli R, Jeejeebhoy KN. 1994. Effects of $\beta$-carotene supplementation on lipid peroxidation in humans. Am J Clin Nutr 59:884-90
  3. Berliner JA, Heinecke JW. 1996. The role of oxidized lipoproteins in atherogenesis. Free Rad Biol Med 20:707-727 https://doi.org/10.1016/0891-5849(95)02173-6
  4. Bieri JG, Tolliver TJ, Catignani GL. 1979. Simultaneous determination of $\alpha$-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. Am J Clin Nutr 32: 2143-2149
  5. Boey PL, Nagao A, Terao J, Tanaka K, Suzuki T, Takama K. 1992. Antioxidant activity of xanthophylls on peroxy radicalmediated phopholipid peroxidation. Biochim Biophys Acta 1126:178-184 https://doi.org/10.1016/0005-2760(92)90288-7
  6. Friedewald WT. Levey RI. 1972. Estimation of the concentration of low-density lipoprotein cholesterol plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499-502
  7. Fuhrman B, Elis A, Aviram M. 1997. Hypocholesterolemic effect of lycopene and $\beta$-carotene is related to suppression of cholesterol synthesis and augmentation of LDL receptor activity in macrophages. Biochem Biophys Res Commun 233:658-662 https://doi.org/10.1006/bbrc.1997.6520
  8. Gaziano JM, Hennekens CH. 1993. The role of beta-carotene in the prevention of cardiovascular disease. Carotenoids in human health. In: Ann. Rev. N.Y. Acad Sci (Canfield LM, Krinsky NI, Olson JA eds) 691:148-155 https://doi.org/10.1111/j.1749-6632.1993.tb26166.x
  9. Glowinska B, Urban M, Koput A. 2002. Cardiovascular risk factors in children with obesity, hypertension and diabetes: Lipoprotein(a) M, Koput A. body mass indva correlate with family history of cardiovascular disease. Eur J Pediatr 161: 511-518 https://doi.org/10.1007/s00431-002-1040-7
  10. Ikeuchi M, Koyama T, Takahashi J, Yazawa K. 2007. Effects of astaxanthin in obese mice fed a highst diet. Biosci Biotechnol Biochem 7:B893-899
  11. Jang JH, Yang KM, Seo JS. 2002. Effect of dietary supplementation of vitamin A or -carotene on oxidative damage induced by acute ethanol administration in rats. J Korean Soc Food Sci Nutr 31:81-86 https://doi.org/10.3746/jkfn.2002.31.1.081
  12. Jang JJ, Devor DE, Logsdon DL, Ward JM. 1992. A 4-week feeding study of ground red chilli (Capsicum annuum) in male B6C3F1 mice. Food Chem Toxicol 30:783-787 https://doi.org/10.1016/0278-6915(92)90080-5
  13. Jialal I, Norkus EP, Cristol L, Grundy SM. 1991. Beta-carotene inhibits the oxidative modification of low density lipoprotein. Biochem Biophys Acta 1086:134-139 https://doi.org/10.1016/0005-2760(91)90164-D
  14. Kang KJ, Choi SS, Han HK, Kim KJ, Kwon SY. 2004. Effects of instant coffee on weight, plasma lipids, leptin, and fat cell size in rats fed on a high fat diet. Korean J Food Sci Technol 36:478-483
  15. Kim MJ, Jang JY, Lee MK, Park JY, Park EM. 1999. Effects of fiber on lipid concentration in hypocholesterolemic rats. Korean J Food Nutr 12:20-25
  16. Krinsky NI, Cornwell DG, Oncley JL. 1958. The transport of vitamin A and carotenoids in human plasma. Arch Biochem Biophys 73:233-246 https://doi.org/10.1016/0003-9861(58)90259-5
  17. Materska M, Perucka I. 2005. Antioxidant activity of the main phenolic compouns isolated from hot pepper fruit (Capsicum annuum L.). J Agric Food Chem 53:1750-1756 https://doi.org/10.1021/jf035331k
  18. Murakami A, Nakashima M, Koshiba T, Maoka T, Nishino H, Yano M, Sumida T, Kim OK, Koshimizu K, Ohigashi H. 2000. Modifying effects of carotenoids on superoxide and nitric oxide generation from stimulated leukocyte. Cancer Letters 149:115-123 https://doi.org/10.1016/S0304-3835(99)00351-1
  19. Nam JH. 1994. Effects of dietary polyunsaturated fatty acids on tocopherol contents and lipid peroxidation of plasma and tissues in rats fed high fat diet. Korean J Food Nutr 7: 373-382
  20. Nicolle C, Cardinault N, Aprikian O, Busserolles J, Grolier P, Rock E, Demigne C, Mazur A, Scalbert A, Remesy. 2003. Effect of carrot intake on cholesterol metabolism and on antioxidant status in cholesterol-fed rat. Eur J Nutr 42:254-261 https://doi.org/10.1007/s00394-003-0419-1
  21. Niki E, Noguchi N, Tsuchihashi H, Gotoh N. 1995. Interaction among vitamin C, vitamin E, and $\beta$-carotene. Am J Clin Nutr 62:1322-1326
  22. Ojima F, Sakamoto H, Ishiguro Y, Terao J. 1993. Consumption of carotenoids in phostosensitized oxidation of human plasma and plasma low-density lipoprotein. Free Radical Biol Med 15:377-384 https://doi.org/10.1016/0891-5849(93)90037-U
  23. Oshima S, Sakamoto H, Ishiguro Y, Terao J. 1997. Accumulation and clearance of capsanthin in blood plasma after the ingestion of paprika juice in men. J Nutr 127:1475-1479
  24. Peto R, Buckley JD, Sporn MB. 1981. Can dietary beta-carotene materially reduce human cancer rates? Nature(Lond) 290: 201-208 https://doi.org/10.1038/290201a0
  25. Seppanen CM, Csallany AS. 2002. The effect of paprika carotenoids on in vivo lipid peroxidation measured by urinary excretion of secondary oxidation products. Nutrition Res 22:1055-1065 https://doi.org/10.1016/S0271-5317(02)00405-0
  26. Sies H, Stahl W, Sundquist A. 1992. Antioxidant functions of vitamins. Ann NY Acad Sci 669:7-20 https://doi.org/10.1111/j.1749-6632.1992.tb17085.x
  27. Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witztum JL. 1989. Beyond cholesterol: Modifications of low density lipoprotein that increase its atherogenicity. N Eng J Med 320:915-924 https://doi.org/10.1056/NEJM198904063201407