한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제23권5호
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  • Pages.423-429
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  • 2010
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지

지골피, 동충하초, 가시오가피 혼합 추출물의 항당뇨 작용

Anti-diabetic Effects of Mixed Extracts from Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus

  • 김대중 (강원대학교 생명건강공학과) ;
  • 김정미 (강원대학교 생명건강공학과) ;
  • 김태혁 (강원대학교 생명건강공학과) ;
  • 백종미 (강원대학교 생명건강공학과) ;
  • 김현숙 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 최면 (강원대학교 생명건강공학과)
  • Kim, Dae-Jung (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Jeong-Mi (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Tae-Hyuck (Department of Bio-Health Technology, Kangwon National University) ;
  • Baek, Jong-Mi (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Hyun-Sook (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Choe, Myeon (Department of Bio-Health Technology, Kangwon National University)
  • 투고 : 2010.06.07
  • 심사 : 2010.10.18
  • 발행 : 2010.10.30
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초록

본 연구에서는 유전적으로 제2형 당뇨를 가진 Goto-Kakizaki 흰쥐의 간 세포액에 함유된 당대사 관련 주요 효소인 glucokinase(GK)와 acetyl-CoA carboxylase(ACC)의 활성 및 $\alpha$-glucosidase 저해 활성을 측정하였다. 사용된 소재로는 지골피(Lycium chinense), 동충하초(Cordyceps militaris), 가시오가피(Acanthopanax senticosus)를 선정하였고, 이를 단독으로 사용하기 보다는 유효성분의 항당뇨 효능에 대한 상승효과를 알아보기 위하여 일정비율로 혼합하였다. 각 소재들을 일정 비율로 혼합한 모든 추출물에서 GK와 ACC의 활성이 증가하였으며, 가시오가피와 동충하초의 혼합 비율이 높아질수록 효소의 활성 증가율도 높아진 것으로 나타났다. 이는 지골피와 가시오가피, 동충하초의 상승작용 때문으로 사료된다. $\alpha$-glucosidase의 저해 활성은 JDG 5(1:6:6) 처리군에서 acarbose 보다 높은 억제율을 나타내었다. 결론적으로 지골피, 가시오가피, 동충하초의 혼합 비율이 1:6:6 일 때 당대사 관련 효소인 GK와 ACC 효소를 활성화시키고, $\alpha$-glucosidase의 활성을 저해하여 흡수된 당의 이용에 유리하게 작용함으로써 항당뇨 효과를 나타낼 수 있을 것으로 사료된다.

In this study, we examined the anti-diabetic activity in vitro by the mixed Korean herb water extracts(JDG) composed of Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus on hepatic glucose-regulating enzyme activities such as glucokinase(GK), acetyl-CoA carboxylase(ACC), and inhibition activity of $\alpha$-glucosidase. The hepatic cytosol fraction of a type II diabetic animal(Goto-Kakizaki rat) was used in GK and ACC activity assays. JDG mixed water extracts significantly increased the hepatic GK and ACC activity. The highest anti-$\alpha$-glucosidase activity was observed in JDG 5 water extract when compared to the acarbose control, inhibitor of $\alpha$-glucosidase. We suggest that Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus mixed water extracts may exert an anti-diabetic activity by enhancing the glucose metabolism and may be used as natural $\alpha$-glucosidase inhibitors in type 2 diabetic conditions.

키워드

참고문헌

  1. Bailey, C.J. 1999. Insulin resistance and antidiabetic drugs. Biochem. Pharmacol. 58:1511-1520. https://doi.org/10.1016/S0006-2952(99)00191-4
  2. Choe, M., D.J. Kim, H.J. Lee, J.K. You, D.J. Seo, J.H. Lee, and M.J. Chung. 2008. A study on the glucose-regulating enzymes and antioxidant activities of water extracts from medicinal herbs. J. Korean Soc. Food Sci. Nutr. 37:542-547(in Korea). https://doi.org/10.3746/jkfn.2008.37.5.542
  3. Colowick, S.P. and N.O. Kaplan. 1981. Methods in enzymology. Lowenstein JM. Academic Press, New York. Vol. 71, p 5-15.
  4. Gao, H. and J. Kawabata. 2005. $\alpha$-Glucosidase inhibition of 6-hydroxyflavones. Part 3 : synthesis and evaluation of 2,3,4-trihydroxybenzoyl-containing flavonoid analogs and 6-amino flavones as $\alpha$-glucosidase inhibitors. Bioorg. Med. Chem. 13:1661-1671. https://doi.org/10.1016/j.bmc.2004.12.010
  5. Ham, I.H., E.S. Jeong, B.H. Lee, and H.Y. Choi. 2008. The study on anti-hypertensive and anti-diabetic effect of Mori ramulus. Korean J. Herbology 23:203-212(in Korea).
  6. Hess, B. and K. Brand. 1974. Methods of Enzymatic Analysis. 2nd ed. Hans UB. Academic Press, New York and London. p. 407-409.
  7. Huang, T.H.W., Q. Yang, M. Harada, J. Uberai, J. Radford, G.Q. Li, J. Yamahara, B.D. Roufogalis, and Y. Li. 2006. Salacia oblonga root improves cardiac lipid metabolism in Zucker diabetic fatty rats: Modulation of cardiac PPAR-$\alpha$-mediated transcription of fatty acid metabolic genes. Toxicology and Applied Pharnacology 210:78-85. https://doi.org/10.1016/j.taap.2005.07.020
  8. Iynedjian, P.B., A. Gjinovei, and A.C. Renold. 1988. Stimulation by insulin of glucokinase gene transcription in liver of diabetic rats. J. Biol. Chem. 83:1998-2001.
  9. Jang, M.J. and Rhee, S.J. 2004. Hypoglycemic effects of pills made of mulberry leaves and silkworm powder in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 33:1611-1617(in Korea). https://doi.org/10.3746/jkfn.2004.33.10.1611
  10. Joo, C.N. and Kim, S.J. 1993. Hypoglycemic action of fat soluble fraction of Panax ginseng C.A. meyer in streptozotocin induced diabetic rats. Korean J. Ginseng Sci. 17:101-108 (in Korea).
  11. Joo, C.N., J.H. Koo, and H.B. Lee. 1993. Study on the hypoglycemic action of fat soluble fraction of Panax ginseng C.A. meyer in streptozotocin induced diabetic rats. Korean J. Ginseng Sci. 17:13-21(in Korea).
  12. Jung, U.J., M.K, Lee, Y.B. Park, M.A. Kang, and M.S. Choi. 2006. Effect of citrus flavonoids on lipid metabolism and glucose-regulating enzyme mRNA levels in type-2 diabetic mice. Int. J. Biochem. Cell Biol. 38:1134-1145. https://doi.org/10.1016/j.biocel.2005.12.002
  13. Kim, D.J., M.J. Chung, J.K. You, D.J. Seo, Kim. J.M. and M. Choe. 2009. Effect of medicinal plant water extracts on glucose-regulating enzyme activities in Goto-Kakizaki rat liver cytosol. J. Korean Soc. Food Sci. Nutr. 38:1331- 1335(in Korea). https://doi.org/10.3746/jkfn.2009.38.10.1331
  14. Kim, O.K. 2008. Antidiabetic and antioxidative effects of Lycii fructus in streptozotocin-induced diabetic rats. J. Kor. Oil Chemist's Soc. 23:73-82(in Korea).
  15. Kim, O.K. 2009. Antidiabetic and antioxidative effect of Lycii Fructus in streptozotocin-induced diabetic rats. Kor. J. Pharmacogen. 40:128-136(in Korea).
  16. Kim, H.S. and M. Choe. 2005. Hypoglycemic effect of Paeciloyces japonica in NIDDM patients. J. Korean Soc. Food Sci. Nutr. 34:821-824(in Korea). https://doi.org/10.3746/jkfn.2005.34.6.821
  17. Kim, H.S., Y.J. Ro, and M. Choe. 2005. Effects of Cordyceps militaris on key enzymes of carbohydrate metabolism. J. Korean Soc. Food Sci. Nutr. 34:1531-1535(in Korea). https://doi.org/10.3746/jkfn.2005.34.10.1531
  18. Kim, S.D., S.I. Lee, and K.O. Shin. 2005. Effect of Acanthopanax senticosus extracts on blood sugar and serum lipid profiles of streptozotocin-induced diabetic rats. J. East Asian Soc. Dietary Life. 15:549-557(in Korea).
  19. Kim, S.Y., H.I. Kim, T.H. Kim, and S.S. Im. 2000. SREBP-1c mediates the insulin dependent hepatic glucokinase expression. J. Biol. Chem. 279:30823-30829.
  20. Lee, K.J., I.H. Ham, Y.M. Bu, H.C. Kim, and H.Y. Choi. 2008. Antidiabetic effect of Glechoma longituba(Nakai) Kupr in diabetic rats induced by streptozotocin. Kor. J. Herbology 23:176-180(in Korea).
  21. Ogawa, S., S. Fujieda, Y. Sakata, M. Ishizaki, S. Hisamatsu, K. Okazaki, Y. Ooki, M. Mori, M. Itoh, and T. Korenaga. 2004. Synthesis and glucosidase inhibitory activity of some N-substituted 5a-carba-$\beta$-fuco- and $\beta$-galactopyranosylamines, and selected derivatives. Bioorg. Med. Chem. 12:6569-6579. https://doi.org/10.1016/j.bmc.2004.09.023
  22. Park, M.J., S.J. Kang, and A.J. Kim. 2009. Hypoglycemic effect of Angelica gigas Nakai extract in Streptozotocininduced diabetic Rats. Kor. J. Food Nutr. 22:246-251. SREBP-1c mediates the insulin dependent hepatic glucokinase expression(in Korea).
  23. Park, S.A., M.S. Choi, M.J. Kim, U.J. Jung, H.J. Kim, K.K. Park, H.J. Noh, H.M. Park, Y.B. Park, J.S. Lee, and M.K. Lee. 2006. Hypoglycemic and hypolipidemic action of Du-zhong (Eucommia ulmoides Oliver) leaves water extract in C57BL/KsJ-db/db mice. J. Ethnopharmacol. 107:412-417 (in Korea). https://doi.org/10.1016/j.jep.2006.03.034
  24. Roman-Ropez, C.R. and J.B. Allred. 1987. Acute alloxan diabetes alters the activity but not the total quantity of acetyl CoA carboxylase in rat liver. J. Nutr. 117:1976-1981. https://doi.org/10.1093/jn/117.11.1976
  25. Sharma, C., R. Manjechwar, and S. Weinhouse. 1963. Effects of diet and insulin on glucose-adenosine triphosphate phosphotransferase of rat liver. J. Biol. Chem. 238:3840-3845.
  26. Takedaa, Y., H. Inouea, K. Honjo, H. Taniokaa, and Y. Daikuharaa. 1967. Dietary response of various key enzymes related to glucose metabolism in normal and diabetic rat liver. Biochim Biophys Acta. 136:214-222. https://doi.org/10.1016/0304-4165(67)90066-9
  27. Task Force Team For Basic Statistical Study of Korean Diabetes Mellitus. 2007. Report of Task Force Team For Basic Statistical Study of Korean Diabetes Mellitus. Diabetes in Korea 2007. 1st ed. Seoul, Goldfishery.
  28. Zhang B.B. and D.E. Moller. 2000. New approaches in the treatment of type 2 diabetes. Curr. Opin. Chem. Biol. 4:461-467. https://doi.org/10.1016/S1367-5931(00)00103-4