Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-diabetic Effects of Mixed Extracts from Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus

지골피, 동충하초, 가시오가피 혼합 추출물의 항당뇨 작용

  • Kim, Dae-Jung (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Jeong-Mi (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Tae-Hyuck (Department of Bio-Health Technology, Kangwon National University) ;
  • Baek, Jong-Mi (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Hyun-Sook (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Choe, Myeon (Department of Bio-Health Technology, Kangwon National University)
  • 김대중 (강원대학교 생명건강공학과) ;
  • 김정미 (강원대학교 생명건강공학과) ;
  • 김태혁 (강원대학교 생명건강공학과) ;
  • 백종미 (강원대학교 생명건강공학과) ;
  • 김현숙 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 최면 (강원대학교 생명건강공학과)
  • Received : 2010.06.07
  • Accepted : 2010.10.18
  • Published : 2010.10.30
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Abstract

In this study, we examined the anti-diabetic activity in vitro by the mixed Korean herb water extracts(JDG) composed of Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus on hepatic glucose-regulating enzyme activities such as glucokinase(GK), acetyl-CoA carboxylase(ACC), and inhibition activity of $\alpha$-glucosidase. The hepatic cytosol fraction of a type II diabetic animal(Goto-Kakizaki rat) was used in GK and ACC activity assays. JDG mixed water extracts significantly increased the hepatic GK and ACC activity. The highest anti-$\alpha$-glucosidase activity was observed in JDG 5 water extract when compared to the acarbose control, inhibitor of $\alpha$-glucosidase. We suggest that Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus mixed water extracts may exert an anti-diabetic activity by enhancing the glucose metabolism and may be used as natural $\alpha$-glucosidase inhibitors in type 2 diabetic conditions.

본 연구에서는 유전적으로 제2형 당뇨를 가진 Goto-Kakizaki 흰쥐의 간 세포액에 함유된 당대사 관련 주요 효소인 glucokinase(GK)와 acetyl-CoA carboxylase(ACC)의 활성 및 $\alpha$-glucosidase 저해 활성을 측정하였다. 사용된 소재로는 지골피(Lycium chinense), 동충하초(Cordyceps militaris), 가시오가피(Acanthopanax senticosus)를 선정하였고, 이를 단독으로 사용하기 보다는 유효성분의 항당뇨 효능에 대한 상승효과를 알아보기 위하여 일정비율로 혼합하였다. 각 소재들을 일정 비율로 혼합한 모든 추출물에서 GK와 ACC의 활성이 증가하였으며, 가시오가피와 동충하초의 혼합 비율이 높아질수록 효소의 활성 증가율도 높아진 것으로 나타났다. 이는 지골피와 가시오가피, 동충하초의 상승작용 때문으로 사료된다. $\alpha$-glucosidase의 저해 활성은 JDG 5(1:6:6) 처리군에서 acarbose 보다 높은 억제율을 나타내었다. 결론적으로 지골피, 가시오가피, 동충하초의 혼합 비율이 1:6:6 일 때 당대사 관련 효소인 GK와 ACC 효소를 활성화시키고, $\alpha$-glucosidase의 활성을 저해하여 흡수된 당의 이용에 유리하게 작용함으로써 항당뇨 효과를 나타낼 수 있을 것으로 사료된다.

Keywords

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