한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제39권1호
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  • Pages.36-41
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  • 2010
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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버섯균사체로 발효시킨 인삼 추출물의 암세포 증식억제 효과

Effect of Fermented Ginseng Extract by Mushroom Mycelia on Antiproliferation of Cancer Cells

  • Kim, Hyun-Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Joung, Eun-Mi (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Jae-Hyun (Dept. of Food and Biotechnology, Chungju National University) ;
  • Yu, Kwang-Won (Dept. of Food and Biotechnology, Chungju National University) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 발행 : 2010.01.30
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초록

상황버섯, 영지버섯 및 노루궁뎅이버섯 균사체로 발효된 인삼추출물이 암세포증식에 미치는 영향을 살펴보기 위하여 위암세포(MKN-45), 대장암세포(HCT116), 유방암세포(MCF-7), 폐암세포(NCIH460), 전립선암세포(PC-3) 및 간암세포(HepG2)에 농도별(0.25～1.5 mg/mL)로 처리하여 암세포 성장억제율을 측정하였다. 전립선암 세포에서의 암세포 증식억제 효과는 영지버섯 균사체발효 인삼추출물이 1.5 mg/mL 농도에서 3.07%로 가장 낮은 생존율을 나타내었으며, 상황버섯 균사체발효 인삼추출물이 35.05%, 노루궁뎅이버섯 균사체발효 인삼추출물이 44.29%의 생존율을 보였다. 폐암세포에 대한 세 가지 버섯균사체발효 인삼추출물의 1.5 mg/mL 농도에서 영지버섯 균사체발효 인삼추출물은 5.31%로 우수한 항암활성을 나타낸 반면, 상황버섯 균사체발효 인삼추출물이 53.52%, 노루궁뎅이버섯 균사체발효 인삼추출물이 35.27%의 생존율을 나타내었다. 이러한 결과로부터 영지버섯 균사체로 발효시킨 인삼추출물이 다른 균사체 인삼발효물보다 다양한 암세포에 대한 성장억제 효과가 우수함을 확인할 수 있었다.

This study was conducted to investigate the effects of fermented ginseng extract by mushroom mycelia on antiproliferation of cancer cells. Phellinus linteus, Ganoderma lucidum, and Hericium erinaceum mycelia were inoculated to ginseng. The effects of fermented ginseng extract on antiproliferation of stomach (MKN-45), colon (HCT116), mammary (MCF-7), lung (NCIH460), prostate (PC-3), and liver (HepG2) cancer cells were investigated by MTT assay. Fermented ginseng extract showed significant antiproliferation effects compared with fresh ginseng extract. Fermented ginseng extract by P. linteus, G. lucidum, and H. erinaceum mycelia showed growth-inhibitory effect of 44.50, 17.75 and 43.98% viability at 1.5 mg/mL on the MKN-45 cell line, 62.86, 3.73, and 54.55% at 1.5 mg/mL on the HCT116 cell line, 41.81, 7.01, and 37.84% at 1.5 mg/mL on the MCF-7 cell line, 53.52, 5.31, and 35.27% at 1.5 mg/mL on the NCIH460 cell line, 35.05, 3.07, and 44.29% at 1.5 mg/mL on the PC-3 cell line, and 59.57, 6.34, and 4.97% at 1.5 mg/mL on the HepG2 cell line, respectively. These results indicated that fermented ginseng by G. lucidum mycelium showed the highest antiproliferation effect against various cancer cells.

키워드

참고문헌

  1. Yu HE, Cho SM, Seo GS, Lee BS, Lee DH, Lee JS. 2006. Screening of bioactive compounds from mushroom Pholiota sp. Korean J Mycol 34: 15-21. https://doi.org/10.4489/KJM.2006.34.1.015
  2. Suzuki S, Oshima S. 1976. Influence of Shitake (Leruinus edodes) on human serum cholesterol. Mushroom Sci 9: 463-467.
  3. Hikino H, Kanno C, Mirin Y, Hayashi T. 1985. Isolation and hypoglycemic activity of Ganoderans A and B, glucans of Ganoderman lucidum fruit bodies. Planta Med 51: 339-340. https://doi.org/10.1055/s-2007-969507
  4. Lee JW, Chung CH, Jeong HJ, Lee KH. 1990. Anticomplementary and antirumor activities of the Ikal extract from the mycelia of Lentinus edodes IY-105. Korean J Appl Microbial Biotechnol 18: 571-577.
  5. Kim HJ, Ahn MS, Kim GH, Kang MH. 2006. Physiological activity/nutrition: antioxidative and antimicrobial activities of Pleurotus eryngii extracts prepared from different aerial part. Korean J Food Sci Technol 38: 799-804.
  6. Song SH, Kim JH, Yang BK, Kim KW. 1996. Anti-complementary polysaccharides produced from submerged mycelial culture of Pleurotus sajocaju. Korean J Mycology 24: 104-110.
  7. Jung IC, Park S, Park KS, Ha CH. 1996. Antioxidative effect of fruit body and mycelia extracts of Pleurotus ostreatus. Korean J Food Sci Technol 28: 464-469.
  8. Hu SY. 1976. The genus Panax (ginseng) in Chinese medicine. Econ Bot 30: 11-28. https://doi.org/10.1007/BF02866780
  9. Lee KY, Park JA, Chung E, Lee YH, Kim SI, Lee SK. 1996. Ginsenoside Rh2 blocks the cell cycle of SK-HEP-1 cells at G1/S boundary by selectively inducing the protein expression Pkip1. Cancer Lett 110: 193-200. https://doi.org/10.1016/S0304-3835(96)04502-8
  10. Lee YS, Kim YR, Kim KM. 1995. Effect of Panax ginseng on morphine-induced immune suppression. J Appl Pharmacol 3: 177-181.
  11. Kim HS, Lee MK. 1989. Effect of ginsenoside on the development of morphine-induced torerance and physical dependence in mice. Korean J Pharmacol 20: 123-127.
  12. Elma ZT, Ilian EZ, Christina IH. 1991. Effect of ginsenoside Rg1 on insulin binding in mice liver and brain membranes. Phytother Res 5: 46-48. https://doi.org/10.1002/ptr.2650050114
  13. Park EM, Kim SJ, Ye EJ, Bae MJ, Jo KC. 2005. Effect of mycelia extracts of mushroom-cultured ginseng by-product on proliferation in cancer cell lines. Korean J Soc Food Sci Nutr 34: 323-329. https://doi.org/10.3746/jkfn.2005.34.3.323
  14. Lee CH, Yang HM, Park SR, Kang YJ. 2007. Major components of mushroom mycelia cultivated with citrus juice processing wastes. Korean J Food Sci Technol 39: 128-132.
  15. Jang KH, Shin KO, Kim SD. 2005. Free amino acid and polysaccharide content of submerges mycelial culture of Fomitopsis pinicola. Korean J Food Preserv 12: 379-386.
  16. Mun HC, Lee HS, Park JH, Kim DH, Lee SY, Seong NS, Bang JG, Jeong HG, Lee HY. 2004. Enhancement of immune activities of Ganoderma lucidum mycelium cultured with garlic enriched medium. Korean J Med Crop Sci 12: 24-30.
  17. Kim MC, Kim JS, Heo MS. 2008. Antibacterial, antioxidant and antitumor activities of mushroom mycelium mixed culture extracts. Korean J Biotechnol Bioeng 23: 158-163.
  18. Kyu SY, Jang HS, Kim JS, Ryu HY, Kim JK, Kwun IS, Sohn HY. 2008. Solid fermentation of medicinal herb using Phellinus baumii mycelium and anti-thrombin and antioxidation activity of its methanol extract. Korean J Microbiol Biotechnol 36: 201-208.
  19. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. 1996. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Bio Pharm Bull 19: 1518-1520. https://doi.org/10.1248/bpb.19.1518
  20. Song JH, Lee HS, Hwang JK, Han JW, Ro JG, Keum DH, Park KM. 2007. Physiological activity of Sarcodon aspratus extracts. Korean J Food Sci Ani Resour 23: 172-179.
  21. Joung EM, Hwang IG, Lee HY, Jeong JH, Yu KW, Jeong HS. 2009. Changes of saponin and $\beta$-glucan content on the cultured ginseng with mushroom mycelia. J Korean Soc Food Sci Nutr 38: 1084-1089. https://doi.org/10.3746/jkfn.2009.38.8.1084
  22. Cha JY, Jeon BS, Moon JC, Yoo JH, Cho YS. 2004. Cytotoxic effect of Inonotus obliquus composition in HCT-15 human colon cancer cells and AGS gastric cancer cells. J Korean Soc Food Sci Nutr 33: 633-640. https://doi.org/10.3746/jkfn.2004.33.4.633
  23. Song JH, Lee HS, Hwang JK, Chung TY, Hong SR, Park KM. 2003. Physiological activities of Phellinus ribis extracts. Korean J Food Sci Technol 35: 690-695.
  24. Kwon SH, Kim CN, Kim CY, Kwon ST, Park KM, Hwangbo S. 2003. Antitumor activities of protein-bound polysaccharide extracted from mycelia of mushroom. Korean J Food Nutr 16: 15-21.
  25. Kim SH, Cha EJ, Hwang TJ. 2004. Studies on antitumor activity and antimicrobial activity of Coriolus versicolor (Fr.) Quel and Ganoderma Lucidum (Fr.) Karst. Korean J Human Ecology 7: 49-58.
  26. Bok JW, Lee SK, Kim BK. 1994. Studies of development of new pharmacologically active components of Ganoderma lucidum. Korean J Biochem 27: 149-153.
  27. Bae WC, Kim YS, Lee JW. 2005. Bioactive substances from Ganoderma lucidum. Korean J Microbiol Biotechnol 33: 75-83.

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  2. Isolation and identification of antiproliferative substances from ginseng fermented using Ganoderma lucidum mycelia vol.24, pp.2, 2015, https://doi.org/10.1007/s10068-015-0074-3
  3. Evaluation of Functional Properties of the Tissue Cultured Wild Ginseng Fermented by Lactobacillus sp. vol.27, pp.6, 2012, https://doi.org/10.7318/KJFC/2012.27.6.743
  4. and their improved bioactivities vol.34, pp.7, 2018, https://doi.org/10.1080/87559129.2018.1424183
  5. 미생물 처리 발효 산삼배양근의 생리활성 변화 vol.24, pp.3, 2010, https://doi.org/10.7783/kjmcs.2016.24.3.191
  6. Antioxidant and Anti-Inflammatory Activity and Cytotoxicity of Ethanol Extracts from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium vol.23, pp.4, 2018, https://doi.org/10.3746/pnf.2018.23.4.326