Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and Identification of a Biphenyl-degrading Bacterium, Pseudomonas sp. DS-94

Biphenyl 분해 미생물 Pseudomonas sp. DS-94의 분리 및 동정

  • Lee, Dae-Sung (POSTECH Ocean Science & Technology Institute, POSTECH) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu)
  • 이대성 (포항공과대학교 해양대학원) ;
  • 정성윤 (대구가톨릭대학교 의생명과학과)
  • Received : 2010.09.04
  • Accepted : 2010.10.12
  • Published : 2010.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Three biphenyl-degrading microorganisms were isolated from polluted soil samples in Sasang-gu, Busan. Among them, isolate DS-94 showing the strong degrading activity was selected. The morphological, physiological, and biochemical characteristics of DS-94 were investigated by API 20NE and other tests. This bacterium was identified as the genus Pseudomonas by 16S rDNA sequencing and designated as Pseudomonas sp. DS-94. The optimum temperature and pH for the growth of Pseudomonas sp. DS-94 were $25^{\circ}C$ and pH 7.0, respectively. This isolate could utilize biphenyl as sole source of carbon and energy. Biphenyl-degrading efficiency of this isolate was measured by HPLC analysis. As a result of biological biphenyl-degradation at high biphenyl concentration (500 mg/L), biphenyl-removal efficiency by this isolate was 73.5% for 7 days.

Keywords

References

  1. 최병순, 국승욱, 김진한, 이동훈, 박철희, 2001, 토양오염 처리기술, 토양오염 개론, 동화기술, 237-244.
  2. Ambrose, A. M., Booth, A. N., DeEds, F., Cox, A. J., 1960, A toxicological study of biphenyl, a citrus fungistat, Food Res., 25, 328-336. https://doi.org/10.1111/j.1365-2621.1960.tb00338.x
  3. Boehncke, A., Koennecker, G., Mangelsdorf, I., Wibbertmann, A., 1999, Biphenyl, Concise International Chemical Assessment Document, 6, World Health Organization, Geneva, Switzerland, 1-31.
  4. Catelani, D., Mosselmans, G., Nienhaus, J., Sorlini, C., Treccani, V., 1970, Microbial degradation of aromatic hydrocarbons used as reactor coolants, Experientia, 26, 922-923.
  5. Dunbar, J., Ticknor, L. O., Kuske, C. R., 2000, Assessment of microbial diversity in four Southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis, Appl. Environ. Microbiol., 66, 2943-2950. https://doi.org/10.1128/AEM.66.7.2943-2950.2000
  6. Furukawa, K., Miyazaki, T., 1986, Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes, J. Bacteriol., 166, 392-398. https://doi.org/10.1128/jb.166.2.392-398.1986
  7. Georgiou, G., Lin, S. C., Sharma, M. M., 1990, Surface active compounds from microorganisms, Biores. Technol., 10, 60-65.
  8. Kumar, S., Tamura, K., Jakobsen, I. B., Nei, M., 2001, MEGA2: Molecular evolutionary genetics analysis software, Bioinformatics, 17, 1244-1245. https://doi.org/10.1093/bioinformatics/17.12.1244
  9. Lars, R., Andrew, J., D., 2007, Biodegradation of biphenyl in a solid–liquid two-phase partitioning bioreactor, Biochem. Eng. J., 36, 195-201. https://doi.org/10.1016/j.bej.2007.02.016
  10. Li, A., Qu, Y. Y., Zhou, J. T., Gou, M., 2009, Isolation and characteristics of a novel biphenyl-degrading bacterial strain, Dyella ginsengisoli LA-4, J. Environ. Sci., 21, 211-217. https://doi.org/10.1016/S1001-0742(08)62253-6
  11. Lunt, D., Evans, W. C., 1970, The microbial metabolism of biphenyl, Biochem. J., 118, 54-55.
  12. MacFaddin, J. F., 1980, Biochemical tests for identification of medical bacteria, 2nd ed., Williams and Wilkins Co., Baltimore, 36-308.
  13. Mondello, F. J., 1989, Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation, J. Bacteriol., 171, 1725-1732. https://doi.org/10.1128/jb.171.3.1725-1732.1989
  14. Moody, J. D., Doerge, D. R., Freeman, J. P., Cerniglia, C. E., 2002, Degradation of biphenyl by Mycobacterium sp. strain PYR-1, Appl. Microbiol. Biotechnol., 58, 364-369. https://doi.org/10.1007/s00253-001-0878-3
  15. Moyer, C. L., Dobbs, F. C., Karl, D. M., 1994, Estimation of diversity and community structure through RFLP distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active hydrothermal vent, Loihi Seamount, Hawaii, Appl. Environ. Microbiol., 60, 871-879.
  16. Pieper, D. H., 2005, Aerobic degradation of polychlorinated biphenyls, Appl. Microbiol. Biotechnol., 67, 170-191. https://doi.org/10.1007/s00253-004-1810-4
  17. Sambrook, J., Fritsch, E. F., Maniatis, T., 1989, Molecular cloning, a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory, New York, 25-28.
  18. Sandmeyer, E. E., 1981, Aromatic Hydrocarbons, Wiley, New York, 3325-3330.
  19. Valle, M., D., Jurado, E., Dachs, J., 2005, The maximum reservoir capacity of soils for persistent organic pollutants: implications for global cycling, Environ. Pollut., 134, 53-164.