Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Analysis of QTLs Related to Resistance to Brown Planthopper in Rice

DH 집단을 이용한 벼멸구 저항성 연관 QTLs 분석

  • Kim, Suk-Man (Department of Agronomy, Kyungpook National University) ;
  • Qin, Yang (Department of Agronomy, Kyungpook National University) ;
  • Sohn, Jae-Keun (Department of Agronomy, Kyungpook National University)
  • 김석만 (경북대학교 농업생명과학대학) ;
  • 친양 (경북대학교 농업생명과학대학) ;
  • 송재근 (경북대학교 농업생명과학대학)
  • Received : 2009.07.27
  • Published : 20090900
⟨ Previous Next ⟩

Abstract

This study was conducted to develop a japonica-type rice cultivar with brown planthopper (BPH) resistance using DNA markers. A doubled haploid (DH) population consisting of 120 pure-lines was established by anther culture of $F_1$ hybrids between 'Samgang', a Tongil type BPH resistance cultivar, and 'Nagdong', a japonica cultivar. To determine the map position of genes responsible for BPH resistance in rice, a genetic map was constructed based on 120 DH lines. A total of 162 molecular markers were classified into 12 linkage groups, covering 1,884 Kosami centimorgan (cM) with an average of 11.6 cM. Five QTLs (qBPR3, qBPR6, qBPR7, qBPR8, and qBPR12) associated with BPH resistance were identified and mapped on chromosomes 3, 6, 7, 8, and 12, respectively, using the genetic map constructed in this study. To analyze the relationship between BPH resistance and agronomic traits, a total of eight QTLs related to the agronomic traits were detected on 12 rice chromosomes. In an analysis of relationships, three QTLs (qBPR3, qBPR7, and qBPR8) showed a linkage with tested agronomic traits. A QTL (qBPR3) located on chromosome 3 (RM282-3023) was closely linked to culm length (qCL3). The QTL (qBPR8) for BPH resistance on the short arm of chromosome 8 also overlapped the region detected in culm length (qCL8).

벼멸구 저항성 유전자의 mapping, 벼멸구 저항성 관련 QTL 분석, 주요 농업형질과 벼멸구 저항성과의 관계 분석 등에 관한 연구를 수행 하여 얻은 결과는 다음과 같다. 벼멸구 저항성을 가진 통일형 벼인 '삼강'과 자포니카형 벼인 '낙동'을 교배하여 양성된 $F_1$의 약을 배양하여 육성된 120 DH 계통을 유전자 지도 작성을 위한 mapping 재료로 이용 하였다. Maker 검정에서 양친에 다양성을 나타낸 SSR 73, STS 89개 등 총 162개의 marker를 이용하여 전체 길이가 1,884 cM이고 평균 marker간 거리가 11.6 cM인 연관지도를 작성하였다. 유전자지도 작성에 이용된 120 DH 계통에 대한 벼멸구 저항성 정도를 조사하여 저항성 관련 QTL을 분석한 바, 3번, 6번, 7번, 8번 그리고 12번 염색체에서 1개씩 총 5개의 QTL을 확인하고 이들을 qBPR3, qBPR6, qBPR7, qBPR8, qBPR12로 명명하였다. 간장, 수장, 수수 및 출수일수에 대한 QTL을 분석하여, 총8개의 QTL이 7개의 염색체에 위치하는 것으로 확인되었다. 벼멸구 저항성과 주요 농업형질관련 QTL과의 연관관계를 분석한 바, 3번 염색체 상의 벼멸구 저항성 QTL(qBPR3)이 간장 및 수수 관련 QTL(qCL3, qNP6)과 연관되어 있었으며, 7번 염색체 상의 qBPR7은 분상질립 관련 QTL qPGC7.1과 qPWBP7.2과 그리고 8번 염색체상의 벼멸구 저항성 QTL 역시 간장관련 QTL(qCL8)과 연관되어 있는 것으로 나타났다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

References

  1. Chen S, Xu CG, Lin XH, and Zhang Q. 2001. Improving bacterial blight resistance of '6078', and elite restorer line of hybrid rice, by molecular marker-assisted selection. Plant Breeding 120:133-137 https://doi.org/10.1046/j.1439-0523.2001.00559.x
  2. Chen H, Wang S, Xing Y, Xu C, Hayes PM, Zhang Q. 2003. Comparative analyses of genomic locations and race specificities of loci for quantitative resistance to Pyricularia grisea in rice and barley. Proc. Natl. Acad. Sci. 100:2544-2549 https://doi.org/10.1073/pnas.0437898100
  3. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on nitrogen source. Scientica Sinica. 5:659-668
  4. Davierwala AP, Reddy APK, Lagu MD, Ranjekar PK, Gupta VS. 2001. Marker assisted selection of bacterial blight resistance genes in rice. Biochemical Genetics. 39:261-278 https://doi.org/10.1023/A:1010282732444
  5. Gad GY, Juvik JA. 2001. Comparison of phenotypic and marker-assisted selection for quantitative traits in sweet corn. Crop Science 41:645-655 https://doi.org/10.2135/cropsci2001.413645x
  6. Fukuta Y, Tamura K, Hirae M, Oya S. 1998. Genetic analysis of resistance to green rice leafhopper (Nephotettix cincticeps UHLER) in rice parental line, norin-PL6, using RFLP markers. Breed. Sci. 48:243-249
  7. Ha WH, Kim KM, and Sohn JK. 2000. Detection of resistance gene to brown planthopper (Nilaparvata lugens Stal.) using RFLP and Isozyme marker in rice. Korean J. Breed. 32(4):319-322
  8. Hirabayashi H, Ideta O, Satoh H, Ohta H, Nemoto H, Imbe T, Vrar DS, Ogawa T. 2002. Location of brown planthopper resistance gene, Bph10(t) in rice. Breeding Science. 52(Suppl.2):509
  9. Hittalmani SA, Parco TV, Mew RS, Zeigler RS, Huang N. 2000. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor. Appl. Genet. 100:1121-1128 https://doi.org/10.1007/s001220051395
  10. Huang N, Parco A, Mew T, Magpantay G, McCouch S, Guiderdoni E, Xu J, Subudhi P, Angeles ER, Khush GS. 1997. RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance on a doubled haploid rice population. Molecular Breed. 3:105-113 https://doi.org/10.1023/A:1009683603862
  11. Huang Z, He G, Shu L, Li X, Q Zhang. 2001. Identification and mapping of two brown planthopper resistance genes in rice. Theor. Appl. Genet. 102: 929-934 https://doi.org/10.1007/s001220000455
  12. Jena KK, Moon HP, Mackill DJ. 2003. Marker assisted selection - A new paradigm in plant breeding. Korean J. Breed. 35(3):133-140
  13. Jena KK, Jeung JU, Lee JH, Choi HC, Brar DS. 2006. High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.). Theor. Appl. Genet. 112:228-297 https://doi.org/10.1007/s00122-005-0194-x
  14. Kaneda C. 1984. Studies on breeding japonica rice resistant to the brown planthopper, Nilaparvata lugens Stal. Bull. Natl. Agric. Res. Cent. 2:1-74
  15. Li ZK, Yu SB, Lafitte HR, Huang N, Courtois B, Hittalmani S, Vijayakumar CHM, Liu GF, Wang GC, Shashidhar HE, Zhuang JY, Zheng KL, Singh VP, Sidhu JS, Srivantaneeyakul S, Khush GS. 2003. QTL environment interactions in rice. I. Heading date and plant height. Theor. Appl. Genet. 108:141-153 https://doi.org/10.1007/s00122-003-1401-2
  16. Manly KF, Olson JM. 1999. Overview of QTL mapping software and introduction to Map Manager QT. Mamm Genome. 10:327-334 https://doi.org/10.1007/s003359900997
  17. Mei HW, Luo LJ, Ying CS, Wang YP, Yu XQ, Guo LB, Paterson AH, Li ZK. 2003. Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two testcross populations. Theor. Appl. Genet. 107:89-101 https://doi.org/10.1007/s00122-003-1192-5
  18. Ramalingam J, Basharat HS, Zhang G. 2002. STS and microsatellite marker-assisted selection for bacterial blight resistance and waxy genes in rice, Oryza sativa L. Euphytica. 127:255-260 https://doi.org/10.1023/A:1020267000418
  19. Sugiura N, Tsuji T, Fujii K, Kato T, Saka N, Touyama T, Hayano-saito Y, Izawa T. 2004. Molecular markerassisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). Breeding research. 6:143-148 https://doi.org/10.1270/jsbbr.6.143
  20. Tsunematsu H, Yasui H, Yoshimura A, Iwata N, Yamasaki M. 1999. Quantitative trait locus mapping of ovicidal response in rice (Oryza sativa L.) against whitebacked planthopper (Sogatella furcifera Horvath). Crop Science. 39:1178-1183 https://doi.org/10.2135/cropsci1999.0011183X003900040038x
  21. Qin Y, Kim SM, Sohn JK. 2009. Genetic amalysis and QTL mapping for grain chalkiness Characteristics of brown rice (Oryza sativa L.). Genes & Genomics 31(2):155-164 https://doi.org/10.1007/BF03191148
  22. Xu XF, Mei H, Luo W, Cheng X, Li ZK. 2002. RFLPfacilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens stal.). Theor. Appl. Genet. 104:248-253 https://doi.org/10.1007/s00122-001-0777-0
  23. Yang H, You A, Yang Z, Zhang F, He R, Zhu L, He G. 2004. High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theor. Appl. Genet. 110:182-191 https://doi.org/10.1007/s00122-004-1844-0
  24. Yeo US, Sohn JK. 1995. Effective screening methods and inheritance of resistance to brown planthopper (Nilaparvata lugens Stal.) in rice. Korean J. Breed. 27(4):372-379
  25. Zou JH, Pan XB, Chen ZX, Xu JY, Lu JF, Zhai WX, Zhu LH. 2000. Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars (Oryza sativa L.), Theor. Appl. Genet. 101:569-573 https://doi.org/10.1007/s001220051517