한국육종학회지 (Korean Journal of Breeding Science)

  • 제41권3호
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  • Pages.205-212
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  • 2009
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  • 0250-3360(pISSN)
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  • 2287-5174(eISSN)
한국육종학회 (The Korean Society of Breeding Science)
권호 표지

Proteomics Approach on Puroindoline Gene of Pre-harvest Sprouting Wheat

  • Kamal, Abu Hena Mostafa (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Park, Cheol-Soo (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Heo, Hwa-Young (Breeding Resource Development, National Institute of Crop Science) ;
  • Chung, Keun-Yook (Department of Agricultural Chemistry, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Cho, Yong-Gu (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Kim, Hong-Sig (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Song, Beom-Heon (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Lee, Chul-Won (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Woo, Sun-Hee (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
  • 투고 : 2009.08.13
  • 발행 : 20090900
⟨ 이전 논문 다음 논문 ⟩

초록

Wheat (Triticum aestivum L.) grain texture is an important determinant of milling properties and end product use. Two linked genes, puroindoline a (PINA) and puroindoline b (PINB), control most of the genetic variation in wheat grain texture. Wheat seed proteins were examined to identify PINA and PINB gene using two pre-harvest sprouting wheat cultivars; Jinpum (resistant) and Keumgang (susceptible).Wheat seed proteins were separated by two-dimensional electrophoresis with IEF gels over pH ranges: pH 3-10. A total of 73 spots were digested with trypsin resulting peptide fragmentation were analyzed by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS). Mass spectra were automatically processed and searched through NCBInr, SWISS-PORT and MSDB database with mono isotopic masses and complete gene sequence were found by UniProt database. Puroindoline a and puroindoline b that is responsible for grain texture related with baking performance and roughness. Two spots were found Pin b (16.7 kDa) and Pin a (16.3 kDa) in Jinpum compare to seven spots were identified Pin a (16.1 kDa, 16.3 kDa) and Pin b (16.7 kDa, 9.5 kDa and 14.4 kDa) in Keumgang. Some selected spots were identified puroindoline like grain softness protein (16.9 kDa, 17 kDa and 18.1 kDa) in Keumgang. Moreover, to gain a better inferring the identification of puroindoline related proteins using proteomics, we accomplished a complete gene sequence of PINA and PINB gene in pre-harvesting sprouting wheat seeds between resistant (Jinpum) and susceptible (Keumgang).

키워드

과제정보

연구 과제 주관 기관 : Rural Development Administration

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