Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Effects of Cactus Extracts on Human Intestinal Microflora

선인장 추출물이 인체 장내미생물에 미치는 영향

  • Ra, Bo-Hyun (Dept. of Food Science and Technology, Chungbuk National Univ.) ;
  • Lee, Woon-Jong (Dept. of Food Science and Technology, Chungbuk National Univ.) ;
  • Cho, Yun-Won (Dept. of Food Science and Technology, Chungbuk National Univ.) ;
  • Kim, Kwang-Yup (Dept. of Food Science and Technology, Chungbuk National Univ.)
  • Received : 2009.02.12
  • Accepted : 2009.06.20
  • Published : 2009.06.30

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Abstract

This study was designed to investigate the effect of cactus (Opuntia ficus-indica var. saboten) extracts on the intestinal bacteria, antioxidative activity and angiotensin -I-converting enzyme(ACE) inhibitory activity. The antimicrobial activities were measured using the 96well-plate method and disc plate method with concentration of 20mg of cactus extract. The stem extract of cactus was inhibitory against Eubacterium limosum, Clostridium perfringens, C. butyricum, C. difficile and Staphylococcus aureus, but was not inhibitory against Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, Streptococcus thermophilus. The fruit extract of cactus showed no inhibition against Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, and Streptococcus thermophilis. Their inhibitory activities were not reduced after heat and pH treatment. Antioxidative effects of cactus extracts showed high total polyphenol and flavonoid contents and high activity against free radical DPPH. The stem and fruit extract of cactus showed strong ACE inhibitory activities of 88.8% and 69.2%, respectively. In conclusion cactus (Opuntia ficus-indica var. saboten) extract might be utilized as a functional food material to control intestinal microflora.

본 연구에서는 인체 내 장내 세균 및 병원성 식중독 세균에 선인장 추출물이 미치는 영향과 항산화, 항고혈압 활성에 대하여 분석하였다. 항균활성은 96well-plate법과 paper disc법을 이용하여 실험하였으며 줄기 추출물의 경우 96well-plate 및 paper disc법의 결과, 장내유해균인 Eubacterium limosum 및 Clostridium perfringens, C. butyricum, C. difficile과 Staphylococcus aureus에 대하여 높은 억제율을 보였으나 Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, Streptococcus thermophilus의 경우 억제율을 나타내지 않았다. 열매 추출물의 경우 장내유해균 및 장내유익균에 Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, and Streptococcus thermophilis에 대하여 억제율을 나타내지 않았다. 또한 줄기 추출물의 경우 열 및 pH에서도 장내미생물에 대한 항균활성에 안정함을 나타내었다. 선인장 추출물의 폴리페놀과 플라보노이드 함량 및 DPPH 전자공여능을 측정한 결과 열처리하지 않은 열매와 줄기 추출물의 폴리페놀은 3.02 mg/g, 5.85 mg/g, 플라보노이드는 1.04 mg/g, 2.1 mg/g, 전자공여능은 87, 88%로 높은 수율을 나타내었다. 선인장 추출물의 항고혈압활성은 줄기 추출물의 경우 88.8% 열매 추출물의 경우 69.2%의 항고혈압활성을 보였으며 상기 결과를 종합할 때, 선인장 줄기추출물은 장내 유해균의 억제 및 유익균의 증진을 통한 장내 균총 개선을 위한 기능성 식품소재로 활용할 수 있다고 판단된다.

Keywords

Acknowledgement

Supported by : 충북대학교

References

  1. Amster D. 1996. Susceptibility testing of antimicrobial in liquid media, antibiotics in laboratory medicine. 4th ed. Williams and Wikins, MD, USA. p 52-111.
  2. Cushman D. W and H. S. Cheung. 1971. Spectrophotometric assey and properties of the angiotensin-converting enzyme of rabbit lung. Bio-chem Pharmacol 20: 1637-1648. https://doi.org/10.1016/0006-2952(71)90292-9
  3. Dewanto V., W, Xianzhong, and R. H. Liu. 2002. Processed sweet corn has higher antioxidant activity. J, Agric, Food Chem, 50: 4959-4964. https://doi.org/10.1021/jf0255937
  4. Floch M. H., M. Wolfman, and R. Doyle. 1980. Fiber and gastrointestinal microecology. J. Clin. Gastroenterol. 2: 175-184. https://doi.org/10.1097/00004836-198006000-00014
  5. Gurrieri S., L. Miceli, C. M. Lanza, F. Tomaselli, R. P. Bonomo, and E. Rizzarellli. 2000. Chemical Charcteriztion of Sicilian prikly pear(Opuntia ficus indica) and perspectives for the storage of its juice. J. Agric. Food Chem. 48: 5424-5431. https://doi.org/10.1021/jf9907844
  6. Peter J., Turnbaugh., Ruth E., Loy., Michael A., Mahowald., Vincent Magrini., Elaine., R. Mardis and I. G. Jeffrey. 2006. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444: 1027-1031. https://doi.org/10.1038/nature05414
  7. Tesoriere L, D. Butera, A. Pintaudi, M. Allgra, and M. A. Livrea. 2004. Supplementation with cactus pear(Opuntia ficus-indica) fruit decrease oxidative stress in healthy human: a comparative study with vitamin C1-3. Am. J Clin Nutr 80: 391-395 https://doi.org/10.1093/ajcn/80.2.391
  8. Cho, I. S., Y. H. Han, G. Y. Lee, and K. Y. Park. 2007. Searching for medicinal plants on improvable effect of intestinal microflora. Korean J. Medicinal Crop Sci. 15: 26-29.
  9. Choi, S. S., and N. J. Ha. 1999. Fecal microflora of mice in relation to diet. Korean J. Micro 35: 128-132.
  10. Chung, H. J. 2000. Antioxidative and antimicrobial activities of Opuntia ficus-indica var. saboten. Korean J. Soc. Food Sci. 16: 160-166.
  11. Do, J. R., I. S. Heo, S. Y. Back, H. S. Yoon, J. H. Jo, Y. M. Kim, K. J. Kim, and S. K. Kim. 2006. Antihypertensive, Antimicrobial and Antifungal Activities of Buckwheat Hydrolysate. Korean J. Food Sci .Technol. 38: 268-272.
  12. Gibson, G. R and M. B. Ronefroid. 1995. Dietary modulation of the human colonic microbiota; infrosucing the concept of prebiotics. J. Nutr. 125: 1401-1402
  13. Ha, M. H., W. P. Park, S. H. Lee, H. J. Heo, and S. H. Cho. 2007. Antimicrobial characteristic of methanolic extracts from Prunus mune byproduct against food spoilage microorganism. Korean J. Food Preserv. 14: 183-187.
  14. Han, B. H., S. K. Woo, and H. K. Shin. 1995. Effect of the water extract of Akebia(Akebia quinata Decaisne) on the growth of Clostridium perfrigens and some intestinal microorganism. Korean J. Appel Micro. Biotech. 23: 633-640.
  15. Jeong, S. J., K. Y. Jun, T. H. Kang, E. B. Ko, and Y. C. Kim. 1999. Flavonoids from the fruits of Opuntia ficus-indica var. saboten. Kor. J. Pharmacogn. 30: 84-86
  16. Kim, M. S., D. C. Lee, J, E. Hong, K. S. Chang, H,. Y. Cho, Y. K. Kwon, and H. Y. Kim. 2000. Antimicrobial effect of ethanol extracts from Korean and Indonesian plants. Korean J. Food Sci. Technol. 32: 949-958.
  17. Kim, T. J., H. D. Yoon, D. S. Lee, S. B. Suh, and D. M. Yeum. 1996. Angiotensin-I converting enzyme inhibitory activity of hot-water extract and enzymematic hydrolysate of freash fish. J. Korean Soc. Food Nutr. 25: 871-877.
  18. Laidlaw, S. A., M. Grosvenor, and J. D. Kopple. 1990 The taurine content of common foodstuffs. J. Parentr. Enteal. Nutr. 14: 183-188 https://doi.org/10.1177/0148607190014002183
  19. Lee, K. S., K. Y. Lee, and C. S. Oh. 2005. Antioxidative effect of the fractions extracted from a Cactus Chennyuncho(Opuntia humihusa). Korean J. Food Sci. Technol. 37: 474-478.
  20. Lee, Y. C., K. H. Hwang, D. H. Han, and S. D. Kim. 1997. Compositions of Opuntia-ficus indica. Korean J. Food Sci. Technol. 29: 847-853.
  21. Maruyama, S., and H. Tanaka. 1989. Angiotensin-I-converting enzyme inhibitors derived from ficus carica. Agric. Biol. chem, 53: 2763-2767
  22. Moon, Y. G., and M S. Her. 2007. Screening of antioxidative and antibacterial activity from methanol extracts of indigenous plants, Jeju-Island. Korean J. Biotechnol Bioeng. 22: 78-83.
  23. Moon, Y. G., C. Y. Song, I. K. Yeo, G. Y. Kim, and M. S. Her. 2008. Antibacterial activities of Suaeda maritima Extract. Journal of life Science 18: 776-781. https://doi.org/10.5352/JLS.2008.18.6.776
  24. Park, E. H., and M. J. Chun. 2001. Wound healing activity of Opuntia fucus-indica. Fitoterapia 72: 165-167. https://doi.org/10.1016/S0367-326X(00)00265-3
  25. Park, E. H., J. H. Kahng, S. H. Lee, and K. H. Shin. 2001. An anti-inflammatory principle from cactus. Fitoterapia 72: 288-290. https://doi.org/10.1016/S0367-326X(00)00287-2
  26. Park, J. Y., J. Y. Jang, M. K. Lee, E. M. Park, M. Y. Kim, D. H. Son, H. Y. Chung, and S. Y. Cho. 2002. Effect of Dandelion(Taraxacum Officinale) extracts on the intestinal microorganism of streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 31: 1112-1118. https://doi.org/10.3746/jkfn.2002.31.6.1112
  27. Seo, K., K. H. Yang, and K. H. Shin., 1999. Antimicrobial and anti oxidative activities of Opuntia-ficus indica var. saboten extracts. Korean J. Postharvest Sci. Technol. 6: 355-359.
  28. Shin, T. K., S. J. Lee, and S. J. Kim. 1998. Effect of Opuntia ficus-indica extract on the activation of immune cells with special references to autoimmune disease models. Korean J. Vet. Pathol. 2: 31-36.
  29. 이시진. 1994. 본초강목. 의성당, 서울.