오리엔탈 종간잡종 나리 미숙배주 배양

Immature Ovule Culture of Lilium Oriental Interspecific crossings

  • 최은경 (전북대학교 농업과학기술연구소) ;
  • 박병모 (전북대학교 환경조경디자인학과)
  • Choi, Eun Gyung (Institute of Agricultural Science & Technology, Chonbuk National University) ;
  • Park, Byung Mo (Environmental Gardening, Chonbuk National University)
  • 투고 : 2009.03.18
  • 심사 : 2009.12.22
  • 발행 : 2009.12.31

초록

오리엔탈나리 (Lilium spp.)를 모본으로 하여 환경적응성이 우수한 종간잡종 나리를 육성하기 위하여 종간 교잡을 실시하였다. 오리엔탈 ${\times}$ 아시아틱 종간교잡은 화주절단수분법에 의해 수분하였지만 평균 29%왜 낮은 결실률을 나타냈다. 오리엔탈 나리 'Rodolfa' ${\times}$ L. asiatic 'Toronto'는 대조구에 비하여 1/4 MS나 1/6 MS배지에서 76.6~78.3%로 발아율이 높았으나, 오리엔탈 나리 'Farolito' ${\times}$ L. asiatic 'Buff Pixie'는 MS와 1/2 MS배지에서 발아율이 86~90% 크게 향상 되었다. 그러나 'Snow Cristal' ${\times}$ Lilium asiatic 'Royal Trinity'는 모든 처리구에서 100%의 발아율을 나타냈다. 종간 잡종배는 배지에 GA $0.5{\sim}1.0mg{\cdot}L^{-1}$ 첨가에 의해서 발아율이 약간 향상되었다. 오리엔탈 나리 'Farolito' ${\times}$ L. asiatic 'Buff Pixie'와 'Belcanto' ${\times}$ L. callosum는 $0.01mg{\cdot}L^{-1}$ NAA와 $0.01mg{\cdot}L^{-1}$ kinetin이 첨가된 MS 배지에서 66.6%와 45.2%로 가장 높은 발아율을 나타냈다.

Interspecific crossing was conducted to obtain hybrid lilly with better quality of adapting to the unfavorable environment using oriental Lilium spp. as a female parent. Average interspecific crossing rate between oriental and asiatic lines by cut-style pollination was as low as 29%. Although the average germination rate of zygotic hybrid embryo between oriental 'Rodolfa' ${\times}$ asiatic 'Toronto' was increased from 76.6% to 78.3%. on 114 or 1/6 strength MS medium compared to the control, the rate of zygotic embryos formation between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' was significantly enhanced up about 86~90% on 1/2 strength MS medium. Meanwhile, germination rate of interspecific hybrid embryo between oriental 'Snow Cristal' ${\times}$ asiatic 'Royal Trinity' showed up 100%. Germination rate of interspecific hybrids slightly increased by addition of $0.5-1.0mg{\cdot}L^{-1}$ GA. Germination rate of zygotic embryos between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' and oriental 'Belcanto' ${\times}$ L. callosum was highest on the MS medium containing $0.01mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ kinetin, as 66.6% and 45.2% respectively.

키워드

참고문헌

  1. Honma, S. and W.L. Summers. 1976. Interspecific hybridization between Brassica napus L. (Napobrassica group) and B. oleracea L. (Botrytis group). J. Amer Soc Hort Sci 101:299-302.
  2. Kim, K. W. and S.K. Sung, 1990. Obtaining of plantlet through immature embryo culture of lilies. J. Kor. Soc. Hort. Sci. 31:423-431.
  3. Kim, S.H., J.S. Lee, D.K. Moon, K.C. Han, and J. Hong. 1998. Immature embryo culture for interspecific hybridization of lilies. J. Kor. Soc. Hort. Sci 39:24-826.
  4. Kim, Y.J., J.H. Lim, Y.P. Hong, K.K. Kim, and S.T. Choi. 1991. I. Studies on hybridization of lily species native in Korea and in vitro culture of immature hybrid embryos. Res. Rept. RDA(H) 33:45-53.
  5. Kim, Y.J., S.M. Park, and J.H. Kim. 2001. Application of in vitro culture methods for overcoming cross-incompatibility in interspecific crosses between L. longiflorum and L. cernuum. Kor. J. Hort. Sci & Technol 19:378-383.
  6. Lee, J.Y. and Y.P. Lim. 2003. Effect of stigmatic and cut-style pollination on selfed and intraspecific seed set in Lilium spp. Korean J. Plant Tissue Culture 30:347-352.
  7. Li, T.H. and Y. Miimi. 1995. A comparison of seed sets in self-intraspecific and interspecific pollination of Lilium species by stigmatic and cut-style pollination methods. J. Japan Soc Hort Sci 64:149-159. https://doi.org/10.2503/jjshs.64.149
  8. Park, C.H., D.S. Ryu, Y.K. Shin, C.B. Park, J.U. Jeong, C.J. Hwang, and M.S. Lee. 1995. Cross compatibility and immature ovule culture of interspecific hybrids in genus Glycine. Korean J. Breed 27:409-416.
  9. Rhee, H.K. 2002. Improvement of breeding efficiency in interspecific hybridization of lilies. Ph. D. thesis, Seoul National University, Seoul, Korea.
  10. Song, C.Y. 2008. Lilium longiflorum 'Charm' as a $F_{1}$ hybrid for pot plant. Flower Res. J. 16:304-308.