화훼연구 (FLOWER RESEARCH JOURNAL)

  • 제17권3호
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  • Pages.165-171
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  • 2009
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  • 1225-5009(pISSN)
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  • 2287-772X(eISSN)
한국화훼학회 (The Korean Society for Floricultural Science)
권호 표지

팔레높시스 화경절편조직 배양에 의한 다신초 형성

Multiple Shoot Formation from Flower Stalk Tissue Culture of Phalaenopsis Hybrids

  • 최진영 (대구가톨릭대학교 플라워디자인학과) ;
  • 고재철 (대구가톨릭대학교 플라워디자인학과)
  • Choi, Jin Young (Department of Floral Design, Catholic University of Daegu) ;
  • Koh, Jae Chul (Department of Floral Design, Catholic University of Daegu)
  • 투고 : 2009.08.04
  • 심사 : 2009.09.24
  • 발행 : 2009.09.30

⟨ 이전 논문 다음 논문 ⟩

초록

팔레높시스 화경의 모든 부위를 사용한 화경절편조직을 기내 배양하여 효율적이고 변이 발생률이 낮은 균일한 식물체를 일시에 대량생산하기 위하여 Hyponex와 생장조절물질 TDZ, BA의 적정 농도를 규명하고자 하였다. 두 공시 품종은 Hyponex, TDZ, BA 농도별로 floral stem, shoot, 다신초의 기관형성 발생에서 차이를 보였다. 팔레높시스의 화경절편조직 배양에 적절한 Hyponex의 농도는 $4g{\cdot}L^{-1}$ 가 가장 효과적이었으며, 다신초 형성에 효과적인 TDZ의 농도는 $0.3mg{\cdot}L^{-1}$이고, BA의 농도는 $5mg{\cdot}L^{-1}$ 이었다. 화경절편조직 배양 시 Hyponex $4g{\cdot}L^{-1}$을 기본배지로 하여 TDZ와 BA의 효과를 비교한 결과 TDZ가 더 효과적이었다.

This experiment was conducted to extend utility of in-vitro culture material stalk and to mass produce uniform plants in Phalaenopsis. The optimal concentration of hyponex medium and plant hormone were examined. The most effective concentration of the hyponex in the flower stalk tissue culture of Phalaenopsis was $4mg{\cdot}L^{-1}$. The most effective concentration of TDZ and BA on the formation of multiple shoots in Phalaenopsis flower stalk culture was $0.3mg{\cdot}L^{-1}$ and $5mg{\cdot}L^{-1}$, respectively. TDZ was more effective for formation of the shoots and multiple shoots than BA at the basic medium with hyponex $4g{\cdot}L^{-1}$.

키워드

참고문헌

  1. Arditti, J. and R. Emst. 1993. Micropropagation of orchids. John Wiley & Sons, Inc. New York. p.467-520.
  2. Been, C.G. 2003a. Continuous production of Phaiaenopsis clones by basal shoot culture. Korean J. Plant Biotechnol. 30: 375-380. https://doi.org/10.5010/JPB.2003.30.4.375
  3. Been, e.G. 2003b. Production of Phaiaenopsis clones by shoot propagation. J. Kor. Flower Res. Soc. 11 : 171-176.
  4. Chen. W.H., T.M. chen, YM. Fu, R.M. Hsieh, and W.S. Chen. 1998. Studies on somaclonal variation in Phaiaenopsis. Plant Cell Reports. 18: 7-13. https://doi.org/10.1007/s002990050523
  5. Fast, G. 1979. Klonvermehrung von phragmipedium Sedenii und Phaiaenopsis hybr. aus Blutenknopspen. Die Orchidee. 30: 241-244.
  6. Homma, Y and T.A sahira. 1985. New means of Phaiaenopsis propagation with internodal sections of flower stalk, J. Japan. Soc. Hort. Sci . 54: 379-387. https://doi.org/10.2503/jjshs.54.379
  7. Hooykass, P.J.J., M.A . Hall, and K.R. Leibbenga. 1999. Biochemistry and molecu lar biology of plant hormones. Elsvier Science.
  8. Ichihashi, S. 1979. Studies on the media for orchid seed germination IV influence of the characteristic of some culture media on the growth of orchid seedling. J. Japan. Soc. Hort. Sci. 48: 345-474. https://doi.org/10.2503/jjshs.48.345
  9. Ichihashi, S. 1992. Micropropagation of Phaiaenopsis through the culture of lateral buds from young flower stalks. Lindleyana. 7: 208-215.
  10. Intuwong, O., J. Kunisaki and Y. Sagawa. 1972. Vegetative propagation of Phalaenopsis by flower stem cuttings. Na Okika O Hawaii-Hawaii Orchid J. 1: 13-18.
  11. Intuwong, O. and Y. Sagawa. 1974. Clonal propagation of Phaiaenopsis by shoot tip culture. Amer Orchid Soc. Bull . 54: 893-895.
  12. Khin TM., C. K. Kim, M.Y Chung, J. S. Park, and J.D. Chung. 2006. Direct induction of multiple shoots from stem tissue of Phaiaenopsis hybrids. Flower Res. J. 14: 69-74.
  13. Kim, M.S., J.S. Eun and YR. Lee. 2001. Effect of in vitro culture condition and lines on growth pattern of lateral bud from nodal cutting of Phaiaenopsis flower stalk. Korean J. Plant Tissue Culture. 28: 189-195.
  14. Kobayashi M., M. Komatuda. and S. Yonai . 1991. Studies on the vegetative propagation of Phalaenopsis through root up culture. Abstr. Japan Soc. Hort. Sci. 59: 664-665.
  15. Morel, G.M. 1960. Producing virus free Cymbidium. Amer. Orchid Soc. Bull. 29: 495-497.
  16. Na, A.S., C.G. Been and B.R. Jeong. 2007. Selection of optimum culture and subculturing conditions for multiple shoot induction and proliferation of Phalaenopsis. Flower Res, J. 15: 139-144.
  17. Roter. G. 1949. A method of vegetat ive propagation of Phalaenopsis species and hybrids. A. O. S. Bull. 18: 738-739.
  18. Sweet, H.R. 1980. The Genus Phalaenopsis. The Orchid Digest Inc.
  19. Tanaka, M. and Y. Sakanish. 1977. Clonal propagation of Phalaenopsis by leaf tissue culture. Amer. Orchid Soc. Bull. 46: 733-737.
  20. Tanaka, M. and Y. Sakanishi. 1978. Factors affecting the growth of in vitro cultured lateral buds from Phalaenopsis flower stalks. Scientia Hort. 8: 169- 173 . https://doi.org/10.1016/0304-4238(78)90022-5
  21. Tokuhara, K. and M. Mii. 1993. Micropropagation of Phalaenopsis and Dorilaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Reports. 13: 7-11.
  22. Tse, A.T.U., Y.R.J. Smith, and W.P. Hackett. 1971. Adventitious shoot formation on Phalaenopsis nodes. Amer. Orchid Soc. Bull. 40: 807-810.