Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Capsular serogroups and toxA gene of Pasteurella multocida isolated from Pneumonic Lung Lesions of Swine

돼지 폐렴병소로부터 분리한 Pasteurella multocida의 capsular serogroup과 toxA gene의 분포

  • Sohn, Jun-Hyung (College of Veterinary Medicine, Kyungpook National University) ;
  • Choi, Seong-Kyoon (College of Veterinary Medicine, Kyungpook National University) ;
  • Cho, Gil-Jae (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2009.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was conducted to investigate the capsular serogroups and distribution of toxA gene of Pasteurella (P.) multocida isolated from pneumonic lung lesions of swine in Korea. A total number of 91 (36.3%) P. multocida isolated from 251 lung lesions. P. multocida isolates were typed for capsular serogroup and toxA gene by polymerase chain reaction. Of the 91 strains, serogroup A and D were 69 strains (75.8%) and 22 strains (24.2%), respectively. Sixty one strains (67.0%) out of 91 strains were detected as toxA gene, and 47 strains (77.0%) and 14 strains (23.0%) belongs to serogroup A and D, respectively.

Keywords

References

  1. Carter GR. Some characteristics of type A strains of Pasteurella multocida. Br Vet J 1958; 114: 356-357
  2. Carter GR, Annau E. Isolation of capsular polysaccharides form colonial variants of Pasteurella multocida. Am J Vet Res 1953; 14: 475-478
  3. Carter GR. Serological classification of pasteurella. Vet Rec 1988; 122: 311
  4. Carter GR. Studies on Pasteurella multocida. IV. Serological types from species other than cattle and swine. Am J Vet Res 1959; 20: 173-175
  5. Cho GJ, Kim BH, Tak RB. Capsular serogrouping and antimicrobial drug susceptibility of Pasteurella multocida isoated from young swine herds, Korea J Vet Res 1989; 29: 487-492
  6. De Angelis PL, Padgett-McCue AJ, Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F, J Biol Chem 2000; 275: 24124-24129 https://doi.org/10.1074/jbc.M003385200
  7. Djordjevic SP, Eamens GJ, Ha H, Walker MJ, Chin JC. Demonstration that Australian Pasteurella multocida isolates from sporadic outbreaks of porcine pneumonia are non-toxigenic (toxA-) and display heterogeneous DNA restriction endonuclease profiles compared with toxigenic isolates from herdswith progressive atrophic rhinitis. J Med Microbiol 1998; 47: 679-688 https://doi.org/10.1099/00222615-47-8-679
  8. Donnio PY, Allardet-Servent A, Perrin M, Escande F, Avril JL. Characterisation of dermonecrotic toxin-producing strains of Pasteurella multocida subsp. multocida isolated from man and swine. J Med Microbiol 1999; 48: 125-131 https://doi.org/10.1099/00222615-48-2-125
  9. Gautam R, Kumar AA, Singh VP, Singh VP, Dutta TK, Shivachandra SB. Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay. Vet Sci 2004; 76: 179-185 https://doi.org/10.1016/j.rvsc.2003.10.005
  10. Hoskins I.C, Thomas LH, Lax LJ. Nasal infection with Pasteurella multocida causes proliferation of bladder epithelium in gnotobiotic pigs. Vet Rec 1997; 140: 22 https://doi.org/10.1136/vr.140.1.22
  11. Kielstein P. On the occurence of toxin-producing Pasteurella multocida strains in atrophic rhinitis and in pneumoniae of swine and cattle. J Vet Med 1986; 33: 418-424 https://doi.org/10.1111/j.1439-0450.1986.tb00052.x
  12. Kim JY, Park JM, Kim NM. Studies on the immunogenicity of Pasteurella multocida isolated from swine in Korea. Res Report of the Rural Development Administration 1986; 28: 77-93
  13. Lax AJ, Grigoriadis AE. Pasteurella multocida toxin: themitogenic toxin that stimulates signalling cascades to regulategrowth and differentiation. Int J Med Microbiol 2001; 291:261-268 https://doi.org/10.1078/1438-4221-00129
  14. Lax AJ, Chanter N, Pullinger GD, Higgins T, Staddon JM, Rozengurt E. Sequence analysis of the potent mitogenic toxin of Pasteurella multocida. FEBS Lett. 1990; 277: 59-64 https://doi.org/10.1016/0014-5793(90)80809-W
  15. Lichtensteiger CA, Steenbergen SM, Lee RM, Polson DD, Vimr ER. Direct PCR analysis for toxigenic Pasteurella multocida. J Clin Microbiol 1996; 34: 3035-3039
  16. Miyashita T. Pasteurella multocida pneumonia. Ryoikibetsu Shokogun Shirizu. 1999; 23: 384-386
  17. Mullan PB, Lax AJ. Pasteurella multocida toxin is a mitogen for bone cells in primary culture. Infect Immun 1996; 64: 959- 965
  18. Muniandy N, Love DN, Mukkur TK. Immunogenicity of purified lipopolysaccharide or protein-oligosaccharide conjugates of Pasteurella multocida type 6:B in mice. Comp. Immunol Microbiol Infect Dis 1998; 21: 257-279 https://doi.org/10.1016/S0147-9571(98)00015-0
  19. Nagai S, Someno S, Yagihashi T. Differentiation of toxigenic from nontoxigenic isolates of Pasteurella multocida by PCR. J Clin Microbiol 1994; 32: 1004-1010
  20. Nakai T, Sawata A, Tsuji M, Samejima Y, Kume K. Purification of dermonecrotic toxin from a sonic extract of Pasteurella multocida SP-72 serotype D. Infect Immun 1984; 46: 429-434
  21. Shin NR, Park JY, Park YH, Yoo HS. Characteristics of Pasteurella multocida isolated frem pneumonic lung lesions of swine; antimicrobial susceptibility, plasmid profile and distribution of toxA. Korean J Vet Res 1999; 39: 1091-1098
  22. Oswald E, Nougayrede JP, Taieb F, Sugai M. Bacterial toxins that modulate host cell-cycle progression. Curr Opin Microbiol 2005; 8: 83-91 https://doi.org/10.1016/j.mib.2004.12.011
  23. Rubies X, Casal J, Pijoan C. Plasmid and restriction endonuclease patterns in Pasteurella multocida isolated from a swine pyramid. Vet Microbiol 2002; 84: 69-78 https://doi.org/10.1016/S0378-1135(01)00440-0
  24. Park JM, Kim JY, Byeon JO. Isolation and serotyping of Pasteurella multocida from pigs respiratory disease. Res. Report of the office of rural deveolpment Korea 1983; 25: 97-104
  25. Petersen SK, Foged NT, Bording A, Nielsen JP, Riemann HK, Frandsen PL. Recombinant derivatives of Pasteurella multocida xin: candidates for a vaccine against progressive atrophic rhinitis. Infect Immun 1991; 59: 1387-1393
  26. Pettit RK, Ackermann MR, Rimler RB. Receptor-mediated binding of Pasteurella multocida dermonecrotic toxin to canine osteosarcoma and monkey kidney (vero) cells. Lab Invest 1993; 69: 94-100
  27. Pijoan C, Lastra A, Ramirez C. Isolation of toxigenic strains of Pasteurella multocida from lungs of pneumoic swine, JAVMA 1984; 185: 522-523
  28. Pullinger GD, Bevir T, Lax AJ. The Pasteurella multocida toxin is encoded within a lysogenic bacteriophage. Mol Microbiol 2004; 51: 255-269 https://doi.org/10.1046/j.1365-2958.2003.03829.x
  29. Rimler RB. Presumptive identification of Pasteurella multocida serogroups A, D and F by capsule depolymerization with mucopolysaccharidases. Vet Rec 1994; 134: 191-192
  30. Rosner H, Grimmecke HD, Knirel YA, Shashkov AS. Hyaluronic acid and a (134)-beta-D-xylan, extracellular polysaccharides of Pasteurella multocida (Carter type A) strain 880. Carbohydr Res 1992; 223: 329-333 https://doi.org/10.1016/0008-6215(92)80032-V
  31. Rozengurt E, Higgins T, Chanter N, Lax AJ, Staddon JM. Pasteurella multocida toxin: potent mitogen for cultured fibroblasts. Proc Natl Acad Sci USA 87. 1990; 123-127 https://doi.org/10.1073/pnas.87.1.123
  32. Rutter JM, Mackenzie A. Pathogenesis of atrophic rhinitis in pigs, a new perspective. Vet Rec 1984; 114: 89-90
  33. Simmon KE, Steadman DD, Durkin S, Baldwin A, Jeffrey WH, Sheridan P, Horton R, Shields MS. Autoclave method for rapid preparation of bacterial PCR-template DNA. J Microbiol Methods 2004; 56: 143-149 https://doi.org/10.1016/j.mimet.2003.10.003
  34. Townsend KM, Boyce JD, Chung JY, Frost AJ, Adler B. Genetic organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system. J Clin Microbiol 2001; 39: 924-929 https://doi.org/10.1128/JCM.39.3.924-929.2001
  35. Zoetendal EG, Ben-Amor K, Akkermans AD, Abee T, de Vos WM. DNA isolation protocols affect the detection limit of PCR approaches of bacteria in samples from the human gastrointestinal tract. Syst Appl Microbiol 2001; 24: 405-410 https://doi.org/10.1078/0723-2020-00060