Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Some Herbal Extracts on Entomopathogenic Nematodes, Silkworm and Ground Beetles

몇 가지 한약재 추출물이 곤충병원성선충과 누에 및 먼지벌레에 미치는 영향

  • Lee, Dong-Woon (Department of Applied Biology, Kyungpook National University) ;
  • Choi, Hyeon-Cheol (Young Il Chemical Co., LTD.) ;
  • Kim, Tae-Su (Department of Applied Biology, Kyungpook National University) ;
  • Park, Jong-Kyun (Department of Applied Biology, Kyungpook National University) ;
  • Park, Jung-Chan (Division of Applied Life Science (BK21), Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Yu, Hwang-Bin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Lee, Sang-Myoung (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Choo, Ho-Yul (Division of Applied Life Science (BK21), Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 이동운 (경북대학교 생물응용학과) ;
  • 최현철 ((주)영일케미컬, 농업생명과학연구원) ;
  • 김태수 (경북대학교 생물응용학과) ;
  • 박종균 (경북대학교 생물응용학과) ;
  • 박정찬 (경상대학교 응용생명과학부(BK21)) ;
  • 유황빈 (경상대학교 응용생명과학부(BK21)) ;
  • 이상명 (국립산림과학원 남부산림연구소) ;
  • 추호렬 (경상대학교 응용생명과학부(BK21))
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Effect of four nematicidal herbal extracts (Daphne genkwa, Eugenia caryophyllata, Quisqualis indica and Zingiber officinale) and 3 acricidal herbal extracts (Pharbitis nil, Xanthium strumarium, and Desmodium caudatum) on entomopathobenic nematodes [Steinernema carpocapsae Pocheon strain (ScP) and Heterorhabditis sp. Gyeongsan strain (HG)], silkworm (Bombyx mori), and ground beetles (Synuchus sp.) were investigated in the laboratory and field. D. genkwa was highly toxic to SCP and HG (100% mortality) at the concentration of 5,000 ppm in X-plate. All the infective juveniles of HG were dead after 3 days by E. caryophyllata and Q. indica. The mortality of ScP and HG was below 10% by D. genkwa, D. caudatum, E. caryophyllata, Q. indica and Z. officinale at the concentration of 1,000 ppm two days after treatment while mortality of HG was 62.8% by D. genkwa at the concentration of 1,000 ppm in X-plate. However, 1,000 ppm had not effect on nematode survival and pathogenicity of ScP in sand column. On the contrary, E. caryophyllata had effect on pathogenicity of HG. Mean number of dead Galleria mellonella larva of HG was 0.5 in E. caryophyllata treatment. Q. indica did not effect silkworm reared on mulberry leaves at the treatment of 1,000 ppm in 10 days after treatment. However, there were 20.0 and 100% mortalities in the treatment of D. genkwa 3 and 10 days after treatment, respectively. The weight of silkworm was low in D. genkwa and did not pupate. The weight of pupa and cocoon were not different in E. caryophyllata, P. nil, Q. indica, X. strumarium and Z. officinale. D. genkwa, E. caryophyllata, P. nil, Q. indica and Z. officinale had no effect on ground beetles, Synuchus sp. in forest soil.

살선충 활성을 지닌 4종의 약용식물 원화(팥꽃나무: Daphne genkwa), 정향(Eugenia caryophyllata), 사군자(Quisqualis indica) 및 건강(생강: Zingiber officinale)과 살비활성을 가진 흑축(나팔꽃: Pharbitis nil), 창이자(도꼬마리: Xanthium strumarium), 청주항(된장풀: Desmodium caudatum) 추출물을 이용하여 천적 미생물인 곤충병원성 선충 [Steinernema carpocapsae 포천계통(ScP)과 Heterorhabditis sp. 경산계통(HG)], 유용곤충인 누에(Bombyx mori) 및 환경지표종인 먼지벌레(Synuchus sp.)에 미치는 영향을 조사하였다. 실내 X-plate에서 한약재 추출물 5,000 ppm 처리에서는 원화의 경우 두 선충 모두 처리 3일 후 100% 치사되었고, HG는 정향과 사군자 처리에서 100% 치사되었다. 1,000 ppm농도에서는 두 선충 모두 처리 2일 후까지 10% 이하의 낮은 치사율을 보였으며 원화 처리에서 HG선충이 62.8%의 치사율을 보였다. Sand column 검정에서 1,000 ppm 농도의 한약재 추출물 처리는 ScP 선충의 생존과 병원성에 차이를 보이지 않았으나 HG선충은 정향 추출물 처리에서 가장 낮은 병원성을 보여 0.5마리의 꿀벌부채명나방 유충만을 치사시켰다. 원화, 정향, 사군자, 흑축, 창이자, 건강이 누에의 생존에 미치는 영향에서는 원화 추출물을 처리한 뽕잎을 급상한 처리구에서는 급상 3일 후에 20%의 치사율을 보였고, 10일째에는 모든 누에가 치사되어 고치와 번데기를 형성하지 못하였다. 누에의 체중도 원화 처리구에서 가장 낮았으며 원화 처리를 제외한 모든 추출물 처리에서는 누에 번데기의 무게와 고치무게가 무처리구와 차이가 없었다. 원화, 정향, 사군자, 흑축, 건강은 먼지벌레에 영향을 미치지 않았다.

Keywords

References

  1. Alexander, S.A. and CM_ Waldenmaier. 2002, Suppression of Pratylenchus penetrans populations in potato and tomato using African marigolds, J. Nematol. 34: 130-134
  2. Bae, K.H. 2000, The medicinal plants of Korea, 654 pp, Kyo-Hak Publishing Co., Ltd. Seoul
  3. Banken, lA.O. and J.D. Stark. 1997, Stage and age influence on the susceptibility of Coccinella septempunctata (Coleoptera: Coccinellidae) after direct exposure to meemix, a neem insecticide, J. Econ. Entomol. 90: 1102-1105 https://doi.org/10.1093/jee/90.5.1102
  4. Cho, I.H. 2006, SAS lecture and statistical consulting. 805 pp, Y oungjin.com. Seoul
  5. Chun, lC., S.E. Kim, lC. Kim and K.Y. Cho. 1999, Identification of natural insecticidal compound in medicinal plants against diamondback moth, The Kor. J. Pesticide Sci. 3: 13-19
  6. Desender, K., M. Dufrene and J.-P. Maelfait. 1994, Long term dynamics of carabid beetles in Belgium: a pre-liminary analysis on the influence of changing climate and land use by means of a database covering more than a century, pp. 247-252. In eds. by Desender, K. et al., Carabid beetle: ecology and evolution. Kluwer Academic Press. Dordrecht
  7. Elbadri, GAA., D.W. Lee, J.e. Park, H.B. Yu, H.Y. Choo, S.M. Lee and T.H. Lim. 2008a, Nematicidal screening of essential oils and herbal extracts against Bursaphelenchus xylophilus, Plant Pathol. J 24: 178-182 https://doi.org/10.5423/PPJ.2008.24.2.178
  8. Elbadri, GAA., D.W. Lee, le. Park, H.Y. Choo and H. H. Kim. 2008b, Inhibition of Meloidogyne incognita egg hatching by herbal extracts, The Kor. J. Pesticide Sci. 12: 155-161
  9. Ferguson, e.S., P.e. Schroeder and E.J. Shields. 1995, Vertical distribution, persistence, and activity of entomopathogenic nematodes (Nematoda: Heterorhabditidae and Steinernematidae) in alfalfa snot beetle (Coleoptera: Curculionidae) infested fields, Environ. Entomol. 24: 149-158 https://doi.org/10.1093/ee/24.1.149
  10. Gokce, A., R. Isaacs and M.E. Whalon. 2006, Behavioural response of Colorado potato beetle (Leptinotarsa decemlineata) larvae to selected plant extracts, Pest Manag. Sci. 62: 1052-1057 https://doi.org/10.1002/ps.1271
  11. Gaugler, R. and H. K. Kaya. 1990, Entomopathogenic nematodes in biological control, CRC Press. Boca Raton, FL. 365 pp
  12. Glazer, I. 2002. Survival biology. pp.l69-187, In Entomopathogenic nematology, ed. by Gaugler, R. 388 pp, CABI Publishing, New York
  13. Hackney, R.W. and O.J. Dickerson. 1975, Marigold, caster bean, and chrysanthemum as controls of Meloidogyne incognita and Pratylenchus alleni, J. Nematol. 7: 84-90
  14. Hiremath, I. G., YJ. Ahn, S.1. Kim, B.R. Choi and J.R. Cho.1995, Insecticidal and arcricidal activities of African plant extracts against the brown planthopper and two-spotted spider mite, Korean J. Appl. Entomol. 34: 200-205
  15. Hwang, I.e., l Kim, H.M. Kim, D.1. Kim, S.G. Kim, S.S. Kim and e. Jang. 2009, Evaluation of toxicity of plant extract made by neem and matrine against main pests and natural enemies, Korean J. Appl. Entomol. 48: 87-94 https://doi.org/10.5656/KSAE.2009.48.1.087
  16. Inomori, Y., K. Takeuchi, K. Babu and M. Kozawa. 1987, Antifeedant and insecticidal activity of daphnodorins A, B, and C. Chern. Pharm. Bull 35: 3931-3934 https://doi.org/10.1248/cpb.35.3931
  17. Isman, M.B. 1999, Neem and related natural products, pp.139-153, In eds. by F.R. Hall, and lJ. Menn. Biopesticides use and delivery, Human Press. Totowa
  18. Kaya, H.K. and R. Gaugler. 1993, Entomopathogenic nematodes, Annu. Rev. Entomol. 38:181-206 https://doi.org/10.1146/annurev.en.38.010193.001145
  19. Kaya, H.K. and S.P. Stock. 1997, Techniques in insect nematology. pp.281-324. In Manual of techniques in insect pathology, ed. by Lacey, L.A. 409 pp. academic Press, San Diego
  20. Kim, J.B. 2005, Pathogen, insect and weed control effects of secondary metabolites from plants, J. Korean Soc. Appl. Chern.48:1-15
  21. Kong, J.O., S.M. Lee, Y.S. Moon, S.G. Lee and Y.l Ahn. 2006, Nematicidal activity of plant essential oils against Bursaphelenchus xylophilus (Nematoda: Aphelenchoidedae), J. Asia-Pacific Entomol. 9:173-178 https://doi.org/10.1016/S1226-8615(08)60289-7
  22. Koul, O. 2004. Neem: a global perspective, pp. 1-19. In eds. by O. Koul and S. Wahab. Neem: today and in the new millennium, Kluwer Academic Press. Dordrecht
  23. Kubota, K., lK. Kim, C.Y. Lee and K. Furata. 2001, Ground beetle fauna in Pinus densiflora forests in Yangyang-gun, Kangwon province, with a special reference to the outbreaks of the pine needle gall-midge (Thecodiplosis japonensis), Jour. Korean For. Soc. 90: 632-642
  24. Kwon, T.S., Y.S. Park, Y.H. Kwon., M.Y. Song, S.C. Shin and J.D. Park. 2003, Effects of aerial pesticide application on arthropod communities in pine forests. Jour. Korean For. Soc.92: 608-617
  25. Lee, D.W., H.Y. Choo and E.J. Choi. 1999, Effects of aqueous solutions of pesticides on survival of entomopathogenic nematodes, Kor. Turfgrass Sci. 13: 213-222
  26. Lee, H.S., G.l Choi, K.Y. Cho, S.G. Lee and Y.J. Ahn. 2000, Fungicidal and insecticidal activities of various grain extracts against five insect pests and six phytopathogenic fungi. The Kor. J. Pesticide Sci. 4: 7-14
  27. Lee, H.P. and G.H. Lee. 1995, Species composition and seasonal abundance of ground beetles (Coleoptera: Carabidae) in three different types of forests, Entomol. Res. Bull. 21: 84-90
  28. Menn, J.l and F.R. Hall. 1999, Biopesticides: present status and future prospects, pp. 1-10. In eds. by F.R. Hall, and lJ. Menn, Biopesticides use and delivery, Human Press, Totowa
  29. Nam, J.H. and Y.I. Ma. 2000, Utilization and resources of insect in the world, 206 pp. Seoul National University Press, Seoul
  30. Nickle, W. R. 1984, Plant and insect nematodes. 890 pp. Marcel Dekker, New York
  31. Perez, E. and E.E. Lewis. 2002, Use of entomopathogenic nematodes to suppress Meloidogyne incognita on greenhouse tomatoes, J. Nematol. 34: 171-174
  32. Prakash, A. and J. Rao. 1997, Botanical pesticides in agriculture,461 pp. CRC Press, Inc. Boca Raton
  33. Ranger, C.M., M.E. Reding, lB. Oliver, II Moyseenko and N.N. Youssef. 2009, Toxicity of botanical formulations to nurseryinfesting white grubs (Coleoptera: Scarabaeidae), J. Econ. Entomol. 102: 304-308 https://doi.org/10.1603/029.102.0140
  34. Samaliev, H.Y., F.1. Andreoglou, SA Elawad, N.G.M. Hague and S.R. Gowen. 2000, The nematicidal effects of the bacteria Pseudomonas oryzihabitans and Xenorhabdus nematophilus on the root-knot nematode Meloidogyne javanica, Nematology. 2:507-514 https://doi.org/10.1163/156854100509420
  35. Smitley, D.R., F.W. Warner and G.W. Bird. 1992, Influence of irrigation and Heterorhabditis bacteriophora on plant-parasitic nematodes in turf, J Nematol. 24: 637-641
  36. Stark, J.D. 2004, Risk assessment of neem products: environmental implications, pp. 97-107, In eds. by O. Koul and S. Wahab. Neem: today and in the new millennium, Kluwer Academic Press. Dordrecht
  37. Woodring, 1. L. and H. K. Kaya. 1988, Steinernematid and Heterorhabditid Nematodes: A Handbook of Biology and Techniques. Arkansas Agricultural Experiment Station, Southern Cooperative Series, Bulletin 331
  38. Yeon, H.S., 1.K. Park, D.W. Lee and K.M. Chung. 2005, Distribution of gronnd-beetles (Coleoptera: Carabidae) in Mt. Gabjangsan, Korea. Kor. Turfgrass Sci, 19: 47-55
  39. Yu, Y.M., E.1. Kang, MJ. Seo, M.G. Kang, H.J. Lee, D.A. Kim, M.R. Gil and Y.N. Yun. 2006, Effects of environment friendly agricultural materials to insect parasitoids in the laboratory, Korean J. Appl. Entomol' 45: 227-234
  40. Zasada, LA. H. Ferris and L. Zheng. 2002, Plant sources of Chinese herval remedies: laboratory efficacy, suppression of Meloidogyne javanica in soil, and phytotoxity assays, J. Nematol 34:124-129

Cited by

  1. Nematicidal Efficacy of Herbal Extracts against Meloidogyne hapla vol.50, pp.4, 2011, https://doi.org/10.5656/KSAE.2011.10.0.57
  2. Eco-Friendly Organic Pesticides (EFOP)-Mediated Management of Persimmon Pests, Stathmopoda masinissa and Riptortus pedestris 2016, https://doi.org/10.5656/KSAE.2016.08.0.016
  3. Preparation of Porous Anti-Insect Repellent Powder Using Spray Drying of Medicinal Herbal Extracts Anti-Insect Repellent Silica Sol vol.26, pp.5, 2015, https://doi.org/10.14478/ace.2015.1067