Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Comparison of Various Methods for Estrus Stage Determination in Bitch

개의 발정 진단 방법에 따른 효율 비교

  • Ko, Young-Jin (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kang, Eun-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Sung-Lim (College of Veterinary Medicine, Gyeongsang National University)
  • 고영진 (경상대학교 수의과대학 수의학과) ;
  • 강은주 (경상대학교 수의과대학 수의학과) ;
  • 이성림 (경상대학교 수의과대학 수의학과)
  • Published : 2009.09.30
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Abstract

In dogs, correct diagnosis of estrus is important and the exact time of ovulation can be determined by variouse methods. Vaginal cytology has commonly used in conjunction with the physical examination, clinical history, vaginoscopy, and hormonal assays to determine the stage of the reproductive cycle. This study was therefore investigated the effectiveness of direct ovulation detector designed by changes of electrical resistance in vaginal mucus following different estrus cycles with several methods; vaginal cytology, concentration of plasma estrogen and progesterone, and direct examination by laparotomy. A total of 12 bitches was selected for the study and observed estrus signs. The bitches were evaluated clinical sign (vulvar swelling and bleeding), cytological examination (keratocyte and RBC), electrical resistance, plasma estrogen and progesterone concentration for estrus assessment. Accuracy of ovulation detection by vaginal cytology was significantly (p<0.05) lower than those by electrical resistance and plasma progesterone concentration, based on the confirmation by laparotomy. Vaginal smear is not confidential method compared to detection of electrical resistance and plasma progesterone concentration at ovulation. Although the value of electrical resistance was varied at the same points of estrus in individuals, ovulation was occurred at the first day which shown the peak of electrical resistance and mating time was third day after peak. In conclusion, ovulation detector designed by changes of electrical resistance is an effective and economic instrument for predicting estrus and ovulation in bitches.

Keywords

References

  1. Babalola GO and Shapiro BH. 1988. Correlation of follicular steroid hormone profiles with ovarian cyclicity in sows. J. Reprod. Fertil. 84:79-87 https://doi.org/10.1530/jrf.0.0840079
  2. Bartlewski PM, Beard AP and Rawlings NC. 1999. The relationship between vaginal mucous impedance and serum concentrations of estradiol and progesterone throughout the sheep estrous cycle. Theriogenology 51:813-827 https://doi.org/10.1016/S0093-691X(99)00029-1
  3. Bell ET, Bailey JB and Christie DW. 1973. Studies on vaginal cytology during the canine oestrous cycle. Res. Vet. Sci. 14:173-179
  4. Bouchard GF, Solorzano N, Concannon PW, Youngquist RS and Bierschwal CJ. 1991. Determination of ovulation time in bitches based on teasing, vaginal cytology, and ELISA for progesterone. Theriogenology 35:603-611 https://doi.org/10.1016/0093-691X(91)90456-N
  5. Boue F, Delhomme A and Chaffaux S. 2000. Reproductive management of silver foxes (Vulpes vulpes) in captivity. Theriogenology 53:1717-1728 https://doi.org/10.1016/S0093-691X(00)00310-1
  6. Christie DW and Bell ET. 1971. Endocrinology of the oestros cycle in the bitch. J. Small. Anim. Pract. 12:383-389 https://doi.org/10.1111/j.1748-5827.1971.tb06245.x
  7. Christie DW and Bell ET. 1972. Studies on canine reproductive behaviour during the normal oestrous cycle. Anim. Behav. 20:621-631 https://doi.org/10.1016/S0003-3472(72)80134-9
  8. Fay J, Mezo T, Solti L, Wolfling A and Abonyi-Toth Z. 2003. Comparison of different methods used for oestrus examination in the bitch. Acta. Vet. Hung. 51:385-394 https://doi.org/10.1556/AVet.51.2003.3.12
  9. Fischer L, Germain G, Florence G and Milhaud C. 1990. Changes in electrical impedance of the vaginal medium during the menstrual cycle of female rhesus monkeys (Macaea mulatta). J. Med. PrimatoI. 19:573-582
  10. Freshman JL. 1991. Clinical approach to infertility in the cycling bitch. Vet. Clin. North. Am. Small. Anim. Pract. 21: 427-435 https://doi.org/10.1016/S0195-5616(91)50052-8
  11. Ginther OJ, Gastal EL, Gastal MO and Beg MA. 2005. Regulation of circulating gonadotropins by the negative effects of ovarian hormones in mares. BioI. Reprod. 73:315-323 https://doi.org/10.1095/biolreprod.105.040253
  12. Haritha S and Rajagopalan G. 2003. Follicular growth, endo-metrial thickness, and serum estradiol levels in spontaneous and clomiphene citrate-induced cycles. lnt. J. GynaecoI. Obstet. 81:287-292 https://doi.org/10.1016/S0020-7292(02)00404-6
  13. Hase M, Hori T, Kawakami E and Tsutsui T. 2000. Plasma LH and progesterone levels before and after ovulation and observation of ovarian follicles by ultrasonographic diagnosis system in dogs. J. Vet. Med. Sci. 62:243-248 https://doi.org/10.1292/jvms.62.243
  14. Hayer P, Gunzel-Apel AR, Ltierssen D and Hoppen HO. 1993. Ultrasonographic monitoring of follicular development, ovulation and the early luteal phase in the bitch. J. Reprod. Fertil. Suppl. 47:93-100
  15. Hiemstra M, Schaefers-Okkens AC, Teske E and Kooistra HS. 2001. The reliability of vaginal cytology in determining the optimal mating time in the bitch. Tijdschr. D. 126:685-689
  16. Jeffcoate lA and England GC. 1997. Urinary LH, plasma LH and progesterone and their clinical correlates in the periovulatory period of domestic bitches. J. Reprod. Fertil. Suppl. 51:267-275
  17. Jeffcoate lA and Lindsay FE. 1989. Ovulation detection and timing of insemination based on hormone concentrations, vaginal cytology and the endoscopic appearance of the vagina in domestic bitches. J. Reprod. Fertil. Suppl. 39:277-287
  18. Jochle Wand Andersen AC. 1977. The estrous cycle in the dog: a review. Theriogenology 7:113-140 https://doi.org/10.1016/0093-691X(77)90240-0
  19. Kutzler MA. 2007. Estrus induction and synchronization in canids and felids. Theriogenology 68:354-374 https://doi.org/10.1016/j.theriogenology.2007.04.014
  20. Lamming GE, Foster JP and Bulman DC. 1979. Pharmacological control of reproduction cycles. Vet. Rec. 104:156-160 https://doi.org/10.1136/vr.104.8.156
  21. Lilley KG, Epping RJ and Hafner LM. 1997. The guinea pig estrous cycle: correlation of vaginal impedance measurements with vaginal cytologic findings. Lab. Anim. Sci. 47: 632-637
  22. Lyimo ZC, Nielen M, Ouweltjes W, Kruip TA and van Eerdenburg FJ. 2000. Relationship among estradiol, cortisol and intensity of estrous behavior in dairy cattle. Theriogenology 53:1783-1795 https://doi.org/10.1016/S0093-691X(00)00314-9
  23. Martinez AR, Zinaman MJ, Jennings VH and Lamprecht VM. 1995. Prediction and detection of the fertile period: the markers. lnt. J. Fertil. Menopausal. Stud. 40:139-155
  24. Menegatos J, Chadio S, Kalogiannis T, Kouskoura T and Kouimtzis S. 2003. Endocrine events during the periestrous period and the subsequent estrous cycle in ewes after estrus synchronization. Theriogenology 59:1533-1543 https://doi.org/10.1016/S0093-691X(02)01205-0
  25. Moghissi KS. 1980. Prediction and detection of ovulation. FertiI. SteriI. 34:89-98 https://doi.org/10.1016/S0015-0282(16)44888-0
  26. Phemister RD, Holst PA, Spano JS and Hopwood ML. 1973. Time of ovulation in the beagle bitch. BioI. Reprod. 8: 74-82 https://doi.org/10.1093/biolreprod/8.1.74
  27. Post K. 1985. Canine vaginal cytology during the estrus cycle. Can. Vet. J. 26:101-104
  28. Ramos SD, Lee JM and Peuler JD. 2001. An inexpensive meter to measure differences in electrical resistance in the rat vagina during the ovarian cycle. J. Appl. Physiol. 91:667-670 https://doi.org/10.1152/jappl.2001.91.2.667
  29. Sokolowski JH, Stover DG and VanRavenswaay F. 1977. Seasonal incidence of estrus and interestrous interval for bitches of seven breeds. J. Am. Vet. Med. Assoc. 171:271-273
  30. Wallace SS, Mahaffey MB, Miller DM, Thompson FN and Chakraborty PK. 1992. Ultrasonographic appearance of the ovaries of dogs during the follicular and luteal phases of the estrous cycle. Am. J. Vet. Res. 53:209-215
  31. Wildt DE, Chakraborty PK, Panko WB and Seager SW. 1978. Relationship of reproductive behavior, serum luteinizing hormone and time of ovulation in the bitch. Biol. Reprod. 18:561-570 https://doi.org/10.1095/biolreprod18.4.561
  32. Wildt DE, Levinson CJ and Seager SW. 1977. Laparoscopic exposure and sequential observation of the ovary of the cycling bitch. Anat. Rec. 189:443-449 https://doi.org/10.1002/ar.1091890305