Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
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Osmotic Cross Second Virial Coefficient ($B_{23}$) of Unfavorable Proteins: Modified Lennard-Jones Potential

  • Choi, Sang-Ha (Division of Chemical Engineering and Molecular Thermodynamics Lab., Hanyang University) ;
  • Bae, Young-Chan (Division of Chemical Engineering and Molecular Thermodynamics Lab., Hanyang University)
  • Published : 2009.10.25
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Abstract

A chromatographic method is used to measure interactions between dissimilar proteins in aqueous electrolyte solutions as a function of ionic strength, salt type, and pH. One protein is immobilized on the surface of the stationary phase, and the other is dissolved in electrolyte solution conditions flowing over that surface. The relative retention of proteins reflects the mean interactions between immobile and mobile proteins. The osmotic cross second virial coefficient calculated by assuming a proposed potential function shows that the interactions of unfavorable proteins depend on solution conditions, and the proposed model shows good agreement with the experimental data of the given systems.

Keywords

References

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