Mycobiology

  • 제37권3호
  • /
  • Pages.211-214
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  • 2009
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Optimal Conditions for Antimicrobial Metabolites Production from a New Streptomyces sp. RUPA-08PR Isolated from Bangladeshi Soil

  • Ripa, F.A. (Pharmaceutical Microbiology and Biotechnology Laboratory, Department of Pharmacy, Rajshahi University) ;
  • Nikkon, F. (Department of Biochemistry and Molecular Biology, Rajshahi University) ;
  • Zaman, S. (Department of chemistry, Rajshahi University) ;
  • Khondkar, P. (Pharmaceutical Microbiology and Biotechnology Laboratory, Department of Pharmacy, Rajshahi University)
  • 발행 : 2009.09.30
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초록

An actinomycete strain was isolated from northern part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. Attempts were made to optimize the culture conditions for the production of antimicrobial metabolites by this strain. Antimicrobial metabolites production was started after 7 days of incubation of culture broth and reached its maximum levels after 10 days and thereafter gradually decreased. The maximum production of antimicrobial metabolites was obtained when the culture medium pH was adjusted to 8. The optimum temperature for antimicrobial metabolites production was $39^{\circ}C$, indicated the new strain as mesophilic organism. Basel medium supplemented with glucose and yeast extract as carbon and nitrogen sources, respectively, was proved to be the best for the production of bioactive metabolites. Maximum production of bioactive metabolites was when NaCl concentration was 1% and among different minerals tested, $K_2HPO_4$ and NaCl showed positive influence on antibiotic production by the strain.

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참고문헌

  1. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. and Turck, M. 1966. Antibiotic susceptibility testing by a standardised single disk method. Am. J. Chn. Pathol. 44:493-496
  2. Bibb, M. J. 2005. Regulation of secondary metabolism in Streptomycetes. Curr. Opin. Microbiol. 8:208-215 https://doi.org/10.1016/j.mib.2005.02.016
  3. Demain, A. L. and Fang, A. 1995. Emerging concepts of secondary metabolism in actinomycetes. Actinomycetology 9:98-117 https://doi.org/10.3209/saj.9_98
  4. Embley, T.M. and Stackebrandt, E. 1994. The molecular Phylogeny and systematics of the actinomycetes. Annu. Rev. Microbiol. 48:257-289
  5. Hossain, M. S., Hossain, M. A., Rahman, M.M.. Mondol, M. A., Bhuiyan, S. A., Gray, A. I., Flores, M. I. and Rashid, M. A. 2004. Amides from the fungu Streptomyces hvgroscopicus and their antimicrobial activity. Phytochemistry 65:2147-2151. https://doi.org/10.1016/j.phytochem.2004.06.010
  6. Jonsbu, E, M,, Intyre, M. and Nielsen, J. 2002. The influence of carbon source and morphology on nystatin production by Streptomyces noursei. J. Biotechnol. 95:133-144 https://doi.org/10.1016/S0168-1656(02)00003-2
  7. Khondkar, P., Islam, M. A. and Rahman,M. M. 1999. Isolation and in vitro antimicrobial screening of 5-hydroxymethylfurfural from a Monocillium species. Raj. Uni. Studies part-2 27:83-87
  8. Kim, S. B., Falconer, C., Williams, E. and Goodfellow, M. 1998. Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocurboxydus so. nov., two moderately thermophilic carboxydotrophic species from soil. Int. J. Syst. Bacteriol. 48:59-68 https://doi.org/10.1099/00207713-48-1-59
  9. Pelczar, M. J., Chan, E. C. S. and Krieg, N. R. 1993. Microbiol ogy: Concepts and Applications. 5th ed. McGraw-Hill, USA
  10. Reiner, R. 1982. Antibiotics: An Introduction. Roche Scientific Service, Switzerland.
  11. Ripa, F. A. 2008. Isolation and characterization of a novel Streptomyces species: Streptomyces profariensis, M. Pharm Thesis, Rajshahi University, BangIadesh.
  12. Shirling, E. B. and Gottlieb, D. 1969. Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies, Int. J. Syst. Bacteriol. 19:391-512 https://doi.org/10.1099/00207713-19-4-391
  13. Tanaka, Y. T. and Mura, S. O. 1993. Agroactive compounds of microbial origin. Annu. Rev. Microbiol. 47:57-87 https://doi.org/10.1146/annurev.mi.47.100193.000421
  14. Wakssman, S. A. and Henrici, A. T. 1943. The nomenclature and classification of the actinomycetes., J. Bacteriol. 46:337-341
  15. Williams, S. T., Goodfellow, M., Alderson, G, Wellington, E. M. H., Sneath, P. H. A. and Sackin, M. J. 1983. Numerical Classification of Streptomyces and related genera. J. Gen. Micmbiol.129:1743-1813

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  4. vol.164, pp.3, 2015, https://doi.org/10.1111/jph.12440
  5. in Laboratory-Scale Bioreactor vol.164, pp.5, 2015, https://doi.org/10.1111/jph.12458
  6. Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity vol.5, pp.1664-302X, 2014, https://doi.org/10.3389/fmicb.2014.00753
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  10. Temperature-Dependent Effects of Cutaneous Bacteria on a Frog’s Tolerance of Fungal Infection vol.9, pp.1664-302X, 2018, https://doi.org/10.3389/fmicb.2018.00410
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