Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Evaluation of Antifungal Activity of Natural Antimicrobials in Fuctional Beverage

기능성 음료의 곰팡이 제어를 위한 천연항균제 활성 평가

  • Lee, Hee-Seok (Department of Food Science and Technology, Chung-Ang University) ;
  • Yeon, Ji-Hye (Department of Food Science and Technology, Chung-Ang University) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Park, Chul-Soo (Kwang-Dong Pharmaceutical Co., Ltd.) ;
  • Woo, Moon-Joe (Kwang-Dong Pharmaceutical Co., Ltd.) ;
  • Lee, Sang-Hun (Kwang-Dong Pharmaceutical Co., Ltd.) ;
  • Kim, Jin-Soo (Kwang-Dong Pharmaceutical Co., Ltd.) ;
  • Lee, Chan (Department of Food Science and Technology, Chung-Ang University)
  • Published : 2009.09.30
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Abstract

Antifungal activity of seven natural antimicrobials, such as $\varepsilon$-polylysine, extract of Yucca shidigera, TLS (vitamin B1 derivative), BMB-FS, chitooligosaccharide, KDSP 001, and KDSP 002 were investigated for their applications in functional beverage. Five fungi including Aspergillus niger, Penicillium citrinum, Rhizopus oryzae, Fusarium moniliforme, and Mucor rouxii were applied as test fungi and mininum inhibitory concentrations (MICs) of antimicrobials were examined. TLS exhibited the strongst antifungal activity among tested antimicrobials and the growth of all fungi was inhibited at 100 ppm. The antifungal activity of BMB-FS appeared different. The growth of Fusarium moniliforme was inhibited by BMB-FS at 100 ppm, but it exhibit antifungal activity on P. citrinum, and M. rouxii at 1000ppm. MICs of TLS appeared to be 60 ppm for four test fungi except A. niger (100 ppm). These results indicate the possible usage of TLS and BMB-FS as natural antimicrobials in functional beverage.

기능성 음료의 저장성을 향상시키고자, 7종류의 천연항균물질($\varepsilon$-Polylysine, 유카추출물, TLS(Vitamin $B_1$ 유도체), BMB-FS(황금추출물), 키토올리고당, KDSP 001, KDSP 002) 곰팡이 성장저해활성을 비교 평가하였다. Aspergillus niger, Penicillium citrimum, Rhizopus oryzae, Fusarium moniliforme, Mucor rouxii, 5 종류의 곰팡이를 상대로 활성을 살펴보았으며, 성장저해활성이 가장 뛰어난 물질의 최소저해농도를 조사하였다. 모든 곰팡이에 대하여 성장 저해활성을 보인 물질은 vitamin $B_1$, 유도체인 TLS 이었으며, 100 ppm 이상의 농도에서는 균사체가 성장하지 못하였다. 황금추출 물 BMB-FS의 경우 F. moniliforme을 상대로 100 ppm이상의 모든 농도에서 성장저해활성을 보였으며 P. citrinum와 M. rouxii의 경우 1,000 ppm이상의 농도가 되어야 균체 성장을 저해하는 것으로 나타났다. 가장 뛰어난 활성을 보여준 TLS의 최소저해농도(MIC)는 A. niger를 제외한 4가지 곰팡이에 대하여 60ppm으로 나타났으며, A. niger에 대한 MIC는 100ppm으로 분석되었다. 이 같은 결과는 기능성 음료의 저장기간을 연장시키는데 중요한 정보를 제공하며, 천연보존료 연구에 도움이 될 것이라 생각된다.

Keywords

References

  1. Park, S.K., Lee, T.S. and Park, S.K. : Estimation of daily dietary intake of food red colors. J. Korean Soc. Food Sci. Nutr. 34, 75-80 (2005) https://doi.org/10.3746/jkfn.2005.34.1.075
  2. Sibel, R. : Natural antimicrobials for the minimal processing offoods. Bibel, R(Ed.). Woodhead Publishing Limited, Cambridge, UK (2003)
  3. Davidson, P.M. and Post, L.S. : Naturally occurring and miscellaneous food antimicrobials. In Antimicrobials in foods. Branen, A.L. Davidson, P.M., Eds, Marcel Dekker, Inc., New York, pp. 371 (1983)
  4. Lewis, R.J. : Their regulatory status their use by the food industry.In Food additives handbook, Robert, W. D. (Ed.), Nostrand Reinhold, New York. p. 3-27 (1989)
  5. 곽종명, 허정림, 허탁 : 음료수용 포장용기의 환경영향, 한국공업화학회지, 2(1), 274-277 (1998)
  6. 이영철, 오세욱, 홍희도 : 식용 가능한 약용식물 추출물의 항균 특성, 한국식품과학회지 ,34, 700-709 (2002)
  7. Beuchat, L.R. and Golden, D.A. : Antimicrobials occuring naturally in food, Food Technol., 43, 134-139 (1989)
  8. Kim, H.Y., Lee, Y.J., Kim, S.H., Hong, K.H., Kwon, Y.K., Lee, J.Y, Ha, S.C., Cho, H.Y., Chang, I.S., Lee, C.W. and Kim, K.S. : Studies on the development of natural preservatives from natural products, Korean J. Food Sci. Technol.,31,1667-1678 (1999)
  9. Lee Y.C, Oh S.W, Hong H.D. Antimicrobial characteristics of edible medicinal herbs extracts, Korean J. Food Sci. Technol., 34, 700-709 (2002)
  10. 김용석, 신동화 : 천연 휘발성 항균물질의 연구현황과 가공물질에의 이용, 한국식품과학회지, 35, 159-165 (2003)
  11. 조성환, 김영록 : 황금추출물의 항균활성. 한국식품영양과학회지, 35(5), 964-968 (2001)
  12. 이종기, 서진종 : 황금탕 및 황금추출물의 식중독 세균에 대한 생육억제 효과. 한국식품영양과학회지, 34(10), 1606-1610 (2005) https://doi.org/10.3746/jkfn.2005.34.10.1606
  13. 지희윤 : 키토산의 Candida albicans와 Trichophyton rubrum에 대한 항진균 작용과 Apoptosis 유도작용, 한국균학회지, 34(2), 119-121 (2006) https://doi.org/10.4489/KJM.2006.34.2.119
  14. Jin, Y.L., Jung,W.J., Kuk, J.H., Kim, J.B., and Park, R.D. : Antifungal and Antioxidative Activities of Yucca smallina Fern, Agr. Chem. Biotechnol., 49(4), 165-170 (2006)
  15. Shima, S., Matsuoka, H., Iwamoto, T. and Sakai, H : Antimicrobial action of a $\varepsilon$-poly-L-lysine, J. Antibiot., 37(11), 1449-1455 (1984) https://doi.org/10.7164/antibiotics.37.1449
  16. Heo, C.Y. and Cho, S.H. : Antimicrobial Activity of Polylysine Produced by Streptomyces sp, J. Agriculture and Life Sci., 36(1), 47-52 (2002)