Journal of the Korean Society of Clothing and Textiles (한국의류학회지)

The Korean Society of Clothing and Textiles (한국의류학회)
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Separation of Chromophoric Substance from Amur Cork Tree Using GC-MS

GC-MS를 이용한 황벽의 색소 성분 분리 거동

  • 안춘순 (인천대학교 패션산업학과)
  • Published : 2009.06.30
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Abstract

Amur cork tree was extracted in methanol with the purpose of investigating the most effective extraction procedure for detecting the chromophore using the GC-MS analysis. Different procedures of waterbath and hotplate extractions were carried out and five different GC-MS instrument parameters including the operating temperatures in the GC capillary column and the MSD scan range were tested for their efficiencies. Berberine was determined by the detection of dihydroberberine at 15.0 min r.t. Hotplate was a better device for extracting amur cork tree than waterbath shaker either with or without presoaking in the room temperature. Water was not an adequate extraction medium for the berberine detection. The most effective GC-MS parameter was Method 4; the initial temperature at $50^{\circ}C$ followed by the temperature increase of $23^{\circ}C$/min until $210^{\circ}C$, then increase of $30^{\circ}C$/min until the final temperature reach at $305^{\circ}C$, then hold for 14 minutes to maintain the total run time 24.12 minutes. The MSD scan range for Method 4 was $35\sim400$m/z.

본 연구는 황벽으로부터 추출하는 염료를 가스 크로마토그라피 질량분석기로 분석할 때 berberine 색소를 가장 효과적으로 검출할 수 있도록 추출방법을 표준화하고 적절한 GC-MS 분석방법을 찾는데 목적을 둔다. 항온진탕기와 가열판을 이용한 다양한 추출법을 적용하였으며 GC 컬럼내 온도조작과 MSD 스캔범위에 따라 5종류의 GC-MS 분석방법의 유효성을 검사하였다. Berberine 표준색소와 추출염료 내 berberine 화합물의 유무는 r.t. 15.0분에서 dihydroberberine의 검출로 확인하였다. 가열판 추출이 항온진탕기 추출보다 더 효과적으로 나타났으며 이는 추출 전 실온에서 사전침지를 한 경우와 하지 않은 경우 모두 동일하였다. 물을 이용한 추출은 berberine 검출에 적합하지 않은 것으로 확인되었다. 본 연구에서 도입한 다섯가지 GC-MS 방법 중 berberine 검출에 가장 효과적인 방법은 Method 4였으며 그 조건은, GC 컬럼내초기온도를 $50^{\circ}C$로 하고 $23^{\circ}C$/분 비율로 온도를 올려 $210^{\circ}C$에 달하면 다시 $30^{\circ}C$/분 비율로 온도를 올려 최종온도 $305^{\circ}C$에 도달했을 때 14분간 온도를 그대로 유지하여 전체 분리시간을 24.12분으로 하며 질량 분석기의 스캔 범위는 $35\sim400$m/z로 하는 것이다.

Keywords

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Cited by

  1. Examination of Berberine Dye using GC-MS after Selective Degradation Treatments vol.33, pp.12, 2009, https://doi.org/10.5850/JKSCT.2009.33.12.2002
  2. GC-MS Analysis of Amur Cork Tree Extract and Its Degradation Products vol.34, pp.6, 2010, https://doi.org/10.5850/JKSCT.2010.34.6.1042