Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Antioxidant Effects of Crotalaria sessiliflora L. Leaf Extracts Fractionated from Each Solvent

활나물 잎 추출물의 분획별 항산화 활성

  • Kim, Tae-Su (Dept. of Food Science and Nutrition, Hoseo University) ;
  • Ko, Sang-Beom (Safety Assesment Center, Korea Testing and Research Institute for Chemical Industry) ;
  • Park, Chun-Geon (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Seong, Ha-Jeong (Institute of Fusion Technology, Hoseo University) ;
  • Kang, Myung-Hwa (Dept. of Food Science and Nutrition, Hoseo University)
  • 김태수 (호서대학교 식품영양학과) ;
  • 고상범 (한국화학시험연구원 안전성 평가본부) ;
  • 박춘근 (농촌진흥청 국립원예특작과학원) ;
  • 성하정 (호서대학교 융합기술연구소) ;
  • 강명화 (호서대학교 식품영양학과)
  • Received : 2008.12.23
  • Accepted : 2009.03.17
  • Published : 2009.04.30
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Abstract

In this study, Crotalaria sessiliflora L., leaf was extracted with a solvent mixture of $CH_2Cl_2$ and $CH_2OH$ (3:1). In order to draw or separate effective components from the leaf extract with high activity, a 1:1 solvent mixture of water and butanol was used after removal of the sugar and fat from the extract. From the decompressed and concentrated butanol layer, 10 small fractions (Fr1, Fr2, Fr3, Fr4, Fr5, Fr6, Fr7, Fr8, Fr9, and Fr10) were obtained using a chromatography column filled with sephadex LH-20. The activity of each fraction was measured by the same method as that used to measure the antioxidant effect of each part(p<0.05). The Fr1, Fr4, and Fr6 fractions showed the highest activity levels(p<0.05). The vital unknown material that vitality exists in the strong butanol fractions of F1, F4 and F6 will be isolated and identified. Currently, we are performing for a single compound from an unknown peak by instrumental analysis.

활나물 잎을 에탄올과 메틸렌 클로라이드 혼합용매(3:1)로 추출하였다. 이중 가장 활성이 높은 유효성분을 추출 및 분리하기 위해서 당과 지방 성분을 제거하고, water와 n-butanol 용매를 1:1 비율로 혼합하여 분획 추출하였다. n-butanol층 은 감압 농축 후, Sephadex LH-20이 충진된 column chromatography를 이용하여 10개(Fr1, Fr2, Fr3, Fr4, Fr5, Fr6, Fr7, Fr8, Fr9, Fr10)의 소 분획물을 얻었다. 분획물의 각종 항산화 활성 결과 Fr1, Fr4, Fr6에서 높은 항산화 활성을 나타내었다(p<0.05). 분획물중 활성이 강한 F1, F4 및 F6에서 미지의 물질을 발견하였으므로 각종 기기분석을 통해 그물질을 동정 중에 있다.

Keywords

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