Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Investigation of Klebsiella pneumoniae Isolates Producing SHV-12 and SHV-11 ${\beta}$-Lactamases in Korean Hospitals

  • Lee, Kyeong-Min (Division of Antimicrobial Resistance, National Institute of Health) ;
  • Yoo, Jae-Il (Division of Antimicrobial Resistance, National Institute of Health) ;
  • Yoo, Yong-Sun (Division of Antimicrobial Resistance, National Institute of Health) ;
  • Yoo, Jung-Sik (Division of Antimicrobial Resistance, National Institute of Health) ;
  • Chung, Gyung-Tae (Division of Antimicrobial Resistance, National Institute of Health) ;
  • Ahn, Tae-In (School of Biological Sciences, Seoul National University) ;
  • Lee, Yeong-Seon (Division of Antimicrobial Resistance, National Institute of Health)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Of 143 clinical isolates of Klebsiella pneumoniae collected from Korean non-tertiary hospitals, 24 (16.8%) showed an extended-spectrum ${\beta}$-lactamase-positive phenotype. PCR and sequence analysis revealed the presence of TEM-116 (n=13), CTX-M-3 (n=5), CTX-M-14 (n=2), CTX-M-15 (n=3), and SHV-12 (n=16). Each of the 24 isolates encoded more than one ${\beta}$-lactamase, and seven isolates (29%) harbored two different SHV-type ${\beta}$-lactamase genes ($bla_{SHV-11}$ and $bla_{SHV-12}$) bounded by insertion sequence IS26 in a single transferable plasmid.

Keywords

References

  1. Clinical and Laboratory Standards Institute. 2006. Performance Standards for Antimicrobial Disk Susceptibility Tests, Ninth Edition. Approved Standard M2-A9. CLSI, Wayne, PA
  2. Clinical and Laboratory Standards Institute. 2007. Performance Standards for Antimicrobial Susceptibility Testing. Seventeenth Informational Supplement M100-S17. CLSI, Wayne, PA
  3. Ford, P. J. and M. B. Avison. 2004. Evolutionary mapping of the SHV beta-lactamase and evidence for two separate IS26- dependent blaSHV mobilization events from the Klebsiella pneumoniae chromosome. J. Antimicrob. Chemother. 54: 69- 75 https://doi.org/10.1093/jac/dkh251
  4. Gouby, A., C. Neuwirth, G. Bourg, N. Bouziges, M. J. Carles- Nurit, E. Despaux, and M. Ramuz. 1994. Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extendedspectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital. J. Clin. Microbiol. 32: 301-305
  5. Hammond, D. S., J. M. Schooneveldt, G. R. Nimmo, F. Huygens, and P. M. Giffard. 2005. blaSHV genes in Klebsiella pneumoniae: Different allele distributions are associated with different promoters within individual isolates. Antimicrob. Agents Chemother. 49: 256-263 https://doi.org/10.1128/AAC.49.1.256-263.2005
  6. Jarlier, V., M. H. Nicolas, G. Fournier, and A. Philippon. 1988. Extended spectrum $\beta$-lactamases conferring transferable resistance to newer $\beta$-lactam agents in Enterobacteriaceae: Hospital prevalence and susceptibility patterns. Rev. Infect. Dis. 10: 867-878 https://doi.org/10.1093/clinids/10.4.867
  7. Jeong, S. H., I. K. Bae, J. H. Lee, S. G. Sohn, G. H. Kang, G. J. Jeon, Y. H. Kim, B. C. Jeong, and S. H. Lee. 2004. Molecular characterization of extended spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean national survey. J. Clin. Microb. 42: 2902-2906 https://doi.org/10.1128/JCM.42.7.2902-2906.2004
  8. Kim, J., H. S. Shin, S. Y. Seol, and D. T. Cho. 2002. Relationship between $bla_{SHV-12}$ and TEX>$bla_{SHV-2a}$ in Korea. J. Antimicrob. Chemother. 49: 261-267 https://doi.org/10.1093/jac/49.2.261
  9. Lee, Y. H., B. Cho, I. K. Bae, C. L. Chang, and S. H. Jeong. 2006. Klebsiella pneumoniae strains carrying the chromosomal SHV-11 $\beta$ lactamase gene produce the plasmid-mediated SHV- 12 extended-spectrum $\beta$-lactamase more frequently than those carrying the chromosomal SHV-1 β-lactamase gene. J. Antimicrob. Chemother. 57: 1259-1261 https://doi.org/10.1093/jac/dkl115
  10. Maslow, J. N., A. M. Slutsky, and R. D. Arbeit. 1993. Application of pulsed field gel electrophoresis to molecular epidemiology, p. 563-572. In D. H. Persing, T. F. Smith, F. C. Tenover, and T. J. White (eds.). Diagnostic Molecular Microbiology: Principles and Applications. American Society for Microbiology, Washington, D.C
  11. Perilli, M. E., B. Dell'Amico, M. R. Segatore, M. R. de Massis, C. Bianchi, F. Luzzaro, et al. 2002. Molecular characterization of extended-spectrum $\beta$-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey. J. Clin. Microbiol. 40: 611-614 https://doi.org/10.1128/JCM.40.2.611-614.2002
  12. Ryoo, N. H., E. C. Kim, S. G. Hong, Y. J. Park, K. Lee, I. K. Bae, E. H. Song, and S. H. Jeong. 2005. Dissemination of SHV-12 and CTX-M-type extended-spectrum beta-lactamase among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. J. Antimicrob. Chemother. 56: 698-702 https://doi.org/10.1093/jac/dki324