Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Seed-Conjugated Polymer Bead for ${\beta}2$-Microglobulin Removal at Neutral pH

  • Kim, Mi-Ra (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kang, Sung-Soo (School of Chemical and Biological Engineering, Seoul National University) ;
  • Myung, Eun-Kyung (School of Chemical and Biological Engineering, Seoul National University) ;
  • Ahn, Min-Koo (School of Chemical and Biological Engineering, Seoul National University) ;
  • Choi, Jeong-Hyun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Paik, Seung-R. (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Yoon-Sik (School of Chemical and Biological Engineering, Seoul National University)
  • Published : 2009.09.30
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Abstract

${\beta}2$-Microglobulin (${\beta}2m$) is known to be a major factor for dialysis-related amyloidosis. We have studied ${\beta}2m$ removal through an aggregation process, which was initiated by a ligand that could catch the ${\beta}2m$ monomer and promote its aggregation into fibril. As a ligand, we have prepared ${\beta}2m$ fibril fragments and used them as a seed. The seed was coupled to PEGylated-PS beads to remove the monomeric ${\beta}2m$ from solution. The ${\beta}2m$ seed-conjugated resin effectively adsorbed the ${\beta}2m$ monomers with a capacity of 3.6 mg/ml via promoting their aggregation into fibrils on the resin at pH 7.4.

Keywords

References

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