Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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$\beta$-Glucan enhanced apoptosis in human colon cancer cells SNU-C4

  • Kim, Mi-Ja (Department of Food and Nutrition, Dongduk Women's University) ;
  • Hong, Se-Young (Department of Food and Nutrition, Dongduk Women's University) ;
  • Kim, Sun-Kyu (Department of Fermented Food Science, Seoul university of Venture & Information) ;
  • Cheong, Chul (Department of Fermented Food Science, Seoul university of Venture & Information) ;
  • Park, Hong-Ju (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Chun, Hye-Kyung (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Jang, Ki-Hyo (Department of Food and Nutrition, Kangwon National University) ;
  • Yoon, Byung-Dae (Molecular Bioprocess Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chul-Ho (Molecular Bioprocess Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Soon-Ah (Department of Fermented Food Science, Seoul university of Venture & Information)
  • Published : 2009.09.30
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Abstract

The apoptotic effect of bacteria-derived $\beta$-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. $\beta$-Glucan of 10, 50, and $100{\mu}g$/mL decreased cell viability in a dose-dependent manner with typical apoptotic characteristics, such as morphological changes of chromatin condensation and apoptotic body formation from TUNEL assay. In addition, $\beta$-glucan ($100{\mu}g$/mL) decreased the expression of Bc1-2 by 0.6 times, whereas the expression of Bax and Caspase-3 were increased by 3.1 and 2.3 times, respectively, compared to untreated control group. Furthermore, the caspase-3 activity in the $\beta$-glucan-treated group was significantly increased compared to those in control group (P < 0.05). Bacterial derived $\beta$-glucan could be used as an effective compound inducing apoptosis in human colon cancer.

Keywords

References

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