Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Simvastatin Induces Osteogenic Differentiation and Suppresses Adipogenic Differentiation in Primarily Cultured Human Adipose-Derived Stem Cells

  • Sun, So-Hyun (Department of Family Medicine, The Catholic University of Korea) ;
  • Lee, Il-Kyu (Department of Family Medicine, The Catholic University of Korea) ;
  • Lee, Jee-Won (Department of Biochemistry, McGill University) ;
  • Shim, In-Sop (Department of Integrative Medicine, The Catholic University of Korea) ;
  • Kim, Se-Hong (Department of Family Medicine, The Catholic University of Korea) ;
  • Kim, Kyung-Soo (Department of Family Medicine, The Catholic University of Korea)
  • Published : 2009.10.31
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Abstract

Recent in vitro and in vivo animal studies have reported that statin, a cholesterol-lowering drug, stimulate osteogenic differentiation. In the present study, we investigated the effect of simvastatin on osteogenic and adipogenic differentiation in primarily cultured human adipose-derived stem cells (hADSCs). The simvastatin treatment significantly increased the positive cell numbers in alkaline phosphatase and von Kossa staining, and enhanced the expression levels of bone morphogenic protein (BMP)-2, core binding factor alpha 1 (cbfa1), collgen type I and osteonectin mRNAs. Lastly, hADSCs were cultured in the adipogenic media with or without simvastatin to examine the effect of simvastatin on adipogenic differentiation. In the RT-PCR analysis, there were notable decreases in mRNA expression of aP1, C/EBP-$\alpha$ and PPAR-$\gamma$ in hADSCs cultivated in simvastatin-added medium, compared to those in simvastatin-free medium. It suggests that the adipogenic differentiation was significantly inhibited by simvastatin treatment. These observations indicate that simvastatin induces osteogenic differentiation and suppresses adipogenic differentiation in hADSCs.

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References

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