한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제13권1호
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  • Pages.51-57
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  • 2009
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

흰쥐황체에서 MCP-1과 큰포식세포아형의 역할에 관한 면역조직화학적 연구

Immunohistochemical Study on Role of the Monocyte Chemoattractant Protein-1 and Macrophage Subpopulations in the Rat Corpora Luteum

  • 조근자 (공주대학교 영상보건대학 보건학부, 공주대학교 건강산업연구소) ;
  • 김원식 (충남대학교 의학전문대학원 해부학교실) ;
  • 김수일 (충남대학교 의학전문대학원 해부학교실, 충남대학교 의학연구소)
  • Cho, Keun-Ja (Division of Health, Kongju National University, Research Institute for Health Industry, College of Visual Image and Health, Kongju National University) ;
  • Kim, Won-Sik (Dept. of Anatomy, Chungnam National University) ;
  • Kim, Soo-Il (Dept. of Anatomy, Chungnam National University, Research Institute for Medical Sciences, School of Medicine, Chungnam National University)
  • 발행 : 2009.03.30
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초록

큰포식세포나 혈관의 내피세포 등에서 분비되는 monocyte chemoattractant protein-1(MCP-1)은 큰포식세포의 활성을 조절하고 황체의 용해시기에는 용해를 개시, 촉진시키는 작용을 하는 것으로 알려져 있다. 그러나 아직 임신 황체나 출산 후의 황체의 발달과 유지에 대한 MCP-1의 작용기전은 확실히 알려져 있지 않다. 난포발달과정에서 큰포식세포의 역할을 알아보기 위해서 흰쥐를 실험동물로 임신시기별, 출산 후 황체에서 TUNEL 염색, ED1, ED2 및 MCP-1에 대한 면역조직화학을 실시하였다. 출산 후 황체에서 큰포식세포의 수가 의미 있게 증가하였으며, 큰포식세포에 대한 ED1, ED2의 면역반응성이 증가하였고, MCP-1의 면역반응성도 크게 증가하였다. 본 연구의 결과 출산 후 황체에서는 큰포식세포가 주로 탐식작용을 하게 되지만, 임신 황체에서는 황체의 구조와 기능을 유지하는데 주로 관여할 것으로 생각된다.

Monocyte chemoattractant protein-1(MCP-1) is released from the macrophages and endothelial cells, regulated luteotropic and luteolytic actions of macrophages and induced luteolysis. However, the mechanisms of MCP-1 on the development and maintenance of pregnant corpora lutea are thoroughly unknown. In this experiment, TUNEL stain, ED1, ED2, and MCP-1 immunohistochemistry on the corpora lutea of pregnant rats were carried out to reveal the role of macrophages in the developing corpora lutea. In the postpartum corpora lutea, the number of macrophages was increased significantly, and the intensity of ED1 and ED2 immunoreactivity in macrophages were increased moderately, and MCP-1 immunoreactivity was also increased. In conclusion, macrophages in the postpartum corpora lutea may exert phagocytic action mainly, and the macrophages in the pregnant corpora lutea maintain the structure and function of lutein cells.

키워드

참고문헌

  1. Bagavandoss P, Wiggins RC, Kunkel SL, Remick DG, Keyes PL (1990) Tumor necrosis factor production and accumulation of inflannnatory cells in the corpus luteum of pseudopregnancy and pregnancy in rabbits. BioI Reprod 42:367-376. https://doi.org/10.1095/biolreprod42.2.367
  2. Bowen 1M, Towns R, Warren JS, Keyes PL (1999) Luteal regression in the normally cycling rat: Apoptosis, monocyte chemoattractant protein-1, and inflannnatory cell involvement. BioI Reprod 60:740-746. https://doi.org/10.1095/biolreprod60.3.740
  3. Brannstrom M, Giesecke L, Moore IC (1994) Leukocyte subpopulations in the rat corpus luteum during pregnancy and pseudopregnancy. BioI Reprod 50:1161-1167. https://doi.org/10.1095/biolreprod50.5.1161
  4. Brannstrom M, Nonnan RJ (1993) Involvement of leukocytes and cytokines in the ovulatory process and corpus luteum function. Hum Reprod 8:1762-1775. https://doi.org/10.1093/oxfordjournals.humrep.a137929
  5. Bukulmez O, Arici A (2000) Leukocytes in ovarian function. Hum Reprod Update 6:1-15. https://doi.org/10.1093/humupd/6.1.1
  6. Fukumatsu Y, Kanabuchi H, Naito M (1992) Effect of macrophages on proliferation of granulosa cells in the ovary in rats. J Reprod Fertil 96:241-249. https://doi.org/10.1530/jrf.0.0960241
  7. Gaytan F, Morales C, Garcia-Pardo L, Raymundo C, Smanchez-Criado JE (1998) Macrophages, cell proliferation, and cell death in the human menstrual corpus luteum. BioI Reprod 59:417-425. https://doi.org/10.1095/biolreprod59.2.417
  8. Grazul-Bilska AT, Redmer DA, Jablonka Shariff A, Biondini ME, Reynolds LP (1995) Proliferation and progesterone production of ovine luteal cells from several stages of the estrous cycle: effects of fibroblast growth factors and luteinizing hormone. Can J Physiol Pharmacol 73:491-500. https://doi.org/10.1139/y95-062
  9. Halme J, Hannnond MG, Syrop CH, Talbert LM (1985) Peritoneal macrophages modulate human granulosa-lutein cell progesterone production. J Clin Endocrinol Metab 61:912-916. https://doi.org/10.1210/jcem-61-5-912
  10. Holt JA (1989) Regulation of progesterone production in the rabbit corpus luteum. BioI Reprod 40:201-208. https://doi.org/10.1095/biolreprod40.2.201
  11. Kelly RW (1994) Pregnancy maintenance and parturition: The role of prostaglandin in manipulating the inflammatory response. Endocr Rev 15:684-706. https://doi.org/10.1210/edrv-15-5-684
  12. McCracken JA, Custer EE, Lamsa JC (1999) Luteolysis : A neuroendocrine-mediated event. Physiol Rev 79:263-323. https://doi.org/10.1152/physrev.1999.79.2.263
  13. Nagaosa K, Shiratsuchi A, Nakanishi Y (2002) Dctennination of cell type specificity and estrous cycle dependency of monocyte chemoattractant protein-1 expression in corpora lutea normally cycling rats in relation to apoptosis and monocyte/macrophage accumulation. BioI Reprod 67:1502-1508. https://doi.org/10.1095/biolreprod.102.005009
  14. Niswender GD, Juengel JA, Silva PJ, Rollyson MK, McIntush EW (2000) Mechanism controlling the function and life span of the corpus luteum. Physiol Rev 80:1-19. https://doi.org/10.1152/physrev.2000.80.1.1
  15. Okuda K, Sakumoto R (2003) Superfamily members in CL function. Reprod BioI Endocrinol 10:95-104.
  16. Pate JL, Keyes PL (2001) Immune cells in the corpus luteum: friends or foes? Reproduction 122:665-676. https://doi.org/10.1530/rep.0.1220665
  17. Penny LA, Armstrong DG, Baxter G, Hogg C, Kindahl H, Bramley T, Watson ED, Webb R (1998) Expresstion of monocyte chemoattractant protein-1 in the bovine corpus luteum around the time of natural luteolysis. BioI Redprod 59:1464-1469. https://doi.org/10.1095/biolreprod59.6.1464
  18. Penny LA (2000) Monocyte chemoattractant protein-1 in luteolysis. J Reprod Fertil 5:63-66.
  19. Rueda BR, Hendry IR, Hendry WJ III, Fong HW, Stormshak F, Slaydenlayden OD, Davis JS (2000) Decreased progesterone level and progesterone receptors antagonists promote apoptotic cell death in bovine luteal cells. BioI Reprod 62:269-276. https://doi.org/10.1095/biolreprod62.2.269
  20. Sakumoto R, Okuda K (2004) Possible actions of tumor necrosis factor-${\alpha}$ in ovarian function. J Reprod Dev 50:39-46. https://doi.org/10.1262/jrd.50.39
  21. Senturk LM, Seli E, Gurierrez LS, Mor G, Zeyneloglu HB, Arici A (1999) Monocyte chemoattractant protein-1 expression in human corpus luteum. Mol Hum Reprod 5:697-702. https://doi.org/10.1093/molehr/5.8.697
  22. Skarzynski DJ, Jaroszewski JJ, Okuda K (2001) Luteotropic mechanisms in the bovine corpus luteum: Role of oxytocin, prostaglandin $F_{2}{\alpha}$, progesterone and noradrenaline. J Reprod Dev 47:125-137. https://doi.org/10.1262/jrd.47.125
  23. Townson DH, Warren JS, Flory eM, Naftalin DM, Keyes PL (1996) Expression of monocyte chemoattractant protein-1 in the corpus luteum of the rat. BioI Reprod 54:513-520. https://doi.org/10.1095/biolreprod54.2.513
  24. Wang LJ, Brannstrom M, Robertson SA, Norman RJ (1992) TNF-${\alpha}$ in the human ovary: presence in follicular fluid and effects on cell proliferation and prostaglandin production. Fertil Steril 58:934-940. https://doi.org/10.1016/S0015-0282(16)55438-7
  25. Wu R, Van der Hoek KH, Ryan NK, Norman RJ, Robker RL (2004) Macrophage contribution to ovarian function. Hum Reprod Update 10:119-133. https://doi.org/10.1093/humupd/dmh011
  26. Zhao Y, Burbach JA, Roby KF, Terranova PF, Brannian JD (1998) Macrophages are the major source of tumor necrosis factor ${\alpha}$ in the porcine corpus luteum. BioI Reprod 59:1385-1391. https://doi.org/10.1095/biolreprod59.6.1385
  27. 김원식, 한승로, 조근자, 김수일, 박창식 (2005) 흰쥐 난소에서 황체내 큰포식세포에 관한 형태학적 연구 : 면역조직화학적 및 투과전자현미경적 연구. 대한해부학회지 38:251-258