KSBB Journal

  • 제24권1호
  • /
  • Pages.59-64
  • /
  • 2009
  • /
  • 1225-7117(pISSN)
  • /
  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Aloe saponaria 캘러스의 열수 추출물 유래 다당의 특성

Polysaccharide Characteristics from Hot Water Extract of Aloe saponaria Callus

  • 백진홍 ((주) 김정문 알로에 과학연구소) ;
  • 김명욱 (강원대학교 생물공학과) ;
  • 강태수 (충북과학대학 바이오식품생명과학과) ;
  • 허원 (강원대학교 생물공학과) ;
  • 이신영 (강원대학교 생물공학과)
  • Baek, Jin-Hong (KJM Aloe R&D Center) ;
  • Kim, Myung-Uk (Department of Bioengineering and Technology, Kangwon National University) ;
  • Kang, Tae-Su (Department od Biofood Science and Biotechnology, Chungbuk Provincial College of Science & Technology) ;
  • Hur, Won (Department of Bioengineering and Technology, Kangwon National University) ;
  • Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
  • 발행 : 2009.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 ${\mu}M$ NAA (${\alpha}$-naphthalene acetic acid) and 3-7 ${\mu}M$ kinetin under incubation in the dark at $25^{\circ}C$ for 6 weeks. The hot water extract ($100^{\circ}C$, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.

키워드

참고문헌

  1. Leung M. Y. K., C. Liu, J. C. M. Koon, and K. P. Fung (2006), Polysaccharide biological response modifiers, lmmunology Letters 105, 101-114 https://doi.org/10.1016/j.imlet.2006.01.009
  2. Talmadge J, J. Chavez， L. Jacobs, C. Munger, T. Chinnah, J. T. Chow, D. Williamson, and K. Yates (2004), Fractionation of Aloe vera L. inner gel, purification and molecular profiling of activity, lnternational lmmunipharmacology 4, 1757-1773
  3. Reynolds, T. and A. C. Dweck (1999), Aloe vera leaf gel: a review update, J. Ethnopharmacol. 68, 3-37 https://doi.org/10.1016/S0378-8741(99)00085-9
  4. Aggarwal D. and K. Bama (2004), Tissue culture propagation of elite plant of Aloe vera Linn., J Plant Biochemistry & Biotechnology 13, 77-79 https://doi.org/10.1007/BF03263197
  5. Chauser-Volfson E. and Y. Gutierman (2004), Using Aloe plants growing in Israel as a source for the cosmetic and medicinal industries and as ornamental plants, Biochemical Systematics and Ecology 28, 825-828 https://doi.org/10.1016/S0305-1978(99)00129-5
  6. Nancy K. Brown (NKB) Aesthetic, Inc., 26 May, 2008
  7. Esua M F. and J. W. Rauwald (2006), Novel bioactive rnaloyl glucans from Aloe vera gel: Isolation, structure elucidation and in vitro bioassays, Carbohydrate Research 341, 355-364 https://doi.org/10.1016/j.carres.2005.11.022
  8. Vanisree M. and H. S. Tsay (2004), Plant cell cultures-An altemative and efficient source for the production of biologically important secondary metabolites, lnternational Journal of Applied Science and Engineering 1, 29-48
  9. Baksha R., M. A. A. Jahan, R. Khatun, and J. L. Munshi (2005), Micropropagation of Aloe barbadensis Mill. through in vitro culture of shoot tip explants, Plant Tissue Cult. & Biotech. 15(2), 121-126
  10. Chukwujekwu J. C., C. W. Fennell, and J. van Staden (2002), Optimization of the tissue culture protocol for the endangered Aloe polyphylla, South Afr. J. Botany 68, 424-429 https://doi.org/10.1016/S0254-6299(15)30368-9
  11. Abrie A. L. and J. van Staden (2001), Micropropagation of the endangered Aloe polyphylla, Plant Growth Regulation 33, 19-23 https://doi.org/10.1023/A:1010725901900
  12. Chaudhuri S. and U. Mukundan (2001), Aloe barbadensis L. micropropagation and characterization of its gel, Phytomorphology 51(2), 155-157
  13. Cho K. H. and J. H. Lee (1998), Preparation process of anthraquinone by culturing Aloe saponaria, Korea Patent No. 10-0148313-0000
  14. Yu C. Y., I. S. Jeon, S. D. Ahn, D. H. Cho, and I. M. Choung (1994), Rapid micropropagation of Aloe arborescens Mill. by meristem culture, J. Oriental Bot. Res, 7(1), 17-22
  15. Kawai K., H. Beppu, T. Koike, K. Fujita, and T. Marunuchi (1993), Tissue culture of Aloe arborescens Miller var. natoalensis Berger, Phytotherapy Research 7, 5-10 https://doi.org/10.1002/ptr.2650070103
  16. Meyer HJ, and J. van Staden (1991). Rapid in vitro propagation of Aloe barbadensis Mill., Plant Cell Tissue and Organ Culture 26, 167-171 https://doi.org/10.1007/BF00039939
  17. Natali, L., I. C. Sanchez, and A. Cavallini (1990), In vitro culture of Aloe barbadensis Mill. Micropropagation from vegetative meristems, Plant Cell Tissue and Organ Culture 20, 71-74 https://doi.org/10.1007/BF00034761
  18. Sanchez I. C., Natali L., and A. Cavallini (1988), In vitro culture of Aloe barbadensis Mill.: Morphogenic ability and nuclear DNA content, Plant Sci. 55, 53-59 https://doi.org/10.1016/0168-9452(88)90041-6
  19. Konishi T. and M. Amano (1985), Callus formation in stamens of Aloe bellatura. Ann. Tsukuba Bot. Garden. 3, 19-26
  20. Yagi A , Y. Shoyama, and I. Nishioka (1983), Formation of tetrahydroanthracene glycosidεs by callus tissue of Aloe saponaria, Phytochemistry 22, 1483-1484 https://doi.org/10.1016/S0031-9422(00)84041-8
  21. Talmadge K. W., K. Keegstra, W. D. Bauer, and P. Albersheim (1973), The structure of plant cell walls. 1. The macromolecular components of the walls of suspension-cultured sycamore cells with a detailed analysis of the pectic polysaccharides, Plant Physiol. 51, 158-173 https://doi.org/10.1104/pp.51.1.158
  22. Gunter E. A. and Yu S. Ovodov (2002), An altemate carbon source for enhancing production of polysaccharides by Silene vulgaris callus, Carbohydrate Research 337, 1641-1645 https://doi.org/10.1016/S0008-6215(02)00079-4
  23. Gunter E. A. and Yu S. Ovodov (2002), Changes in cell wall polysaccharides of Silene vulgaris callus during culture, Phytochemistry 59, 703-708 https://doi.org/10.1016/S0031-9422(02)00041-9
  24. Gunter E. A., and Yu S. Ovodov (2003), Production of polysaccharides by Silene vulgaris callus culture of depending on carbohydrates of the medium, Bochemistry (Moscow) 68(8), 882-889 https://doi.org/10.1023/A:1025751015684
  25. Gunter E. A., O. V. Popeiko, and Yu S. Ovodov (2004), Isolation of polysaccharides from the callus culture of Lemna minor L., Applied Biochemistry and Mìcrobiology 40(1), 80-83 https://doi.org/10.1023/B:ABIM.0000010359.68528.fe
  26. Gunter E. A, O. V. Popeiko, and Yu S. Ovodov (2007), Modification of polysaccharides from callus culture of Silene vulgaris (M.) G. using carbohydrases in vitro. Bochemistry (Moscow) 72(9), 1008-1015 https://doi.org/10.1134/S0006297907090131
  27. Bushneva O. A., R. G. Ovodova, A. S. Shashkov, A. O. Chizhov, Günter E. A., and Yu S. Ovodov (2006), Structural studies of arabinigalactan and pectin from Silene vulgaris (M.) G. callus, Bochemistry (Moscow) 71(6), 644-651 https://doi.org/10.1134/S0006297906060083
  28. Yagi A., S. J. Shibata, J. Nishioka, S. Iwadare, and Y. Ishida (1982), Cardiac stimulant action of constituents of Aloe saponaria. J. Pharmaceut. Sci. 71(7), 739-741 https://doi.org/10.1002/jps.2600710705
  29. Sampedroa M. C., R. L. Artolab, M. Muraturec, D. Muratureb, Y. Ditamoa, G. A Rotha, and S. Kivatinitza (2004), Mannan from Aloe saponaria inhibits tumoral cell activation and proliferation, International Immunopharmacology 4, 411-418 https://doi.org/10.1016/j.intimp.2003.12.016
  30. McAnalley, B. H. and G. Prairie (1990), Process for preparation of aloe products. US patent 4,957,907
  31. Gowda, D., B. Neelisiddaiah, and Y. Anjaneyalo (1980), Structural studies of polysaccharides from Aloe saponaria and Aloe vanbalenii, Carbohydrate Res. 83, 402-405 https://doi.org/10.1016/S0008-6215(00)84556-5
  32. Murashige, T. and F. Skoog (1962), A revised medium for rapid growth and bioassay with tobacco tissue culture, Physiologia Plantarum 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  33. Dubois M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith (1956), Colorimetric method for determination of sugars and related substances, Anal. Chem. 28, 350-356 https://doi.org/10.1021/ac60111a017
  34. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951), Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193, 265-275
  35. AOAC. (1995), Official methods of analysis. 16th(ed). Association of Official Analytical Chemists, Washington D.C., USA
  36. Femenia A., E. S. Sanchez, S. Simal, and C. Rossello (1999), Compositional features of polysaccharides from Aloe vera (Aloe barbadensis Miller) plant tissues, Carbohydr. Polym. 39, 109-117 https://doi.org/10.1016/S0144-8617(98)00163-5