KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Selection and Mechanism of Anti-Obesity Agents from Natural Products Based on Anti-Angiogenesis

신생혈관형성억제작용을 기반으로 한 항비만제제의 선별 및 작용기전

  • Shin, Jin-Hyuk (Department of Biotechnology, Chonnam National University) ;
  • Lee, Jin-Hee (Dept. of Culture Biotechnology, Chonnam National University) ;
  • Kang, Kyeong-Wan (Dept. of Culture Biotechnology, Chonnam National University) ;
  • Hwang, Jae-Ho (Dept. of Culture Biotechnology, Chonnam National University) ;
  • Han, Kyeong-Ho (Dept. of Culture Biotechnology, Chonnam National University) ;
  • Shin, Tai-Sun (Food Science and Nutrition, Chonnam National University) ;
  • Kim, Min-Yong (Dept, of Refrigeration Eng., Chonnam National University) ;
  • Kim, Jong-Deog (Department of Biotechnology, Chonnam National University)
  • 신진혁 (전남대학교 생명화학공학부 생명산업공학과) ;
  • 이진희 (전남대학교 해양기술학부) ;
  • 강경완 (전남대학교 해양기술학부) ;
  • 황재호 (전남대학교 해양기술학부) ;
  • 한경호 (전남대학교 해양기술학부) ;
  • 신태선 (전남대학교 영양식품학) ;
  • 김민용 (전남대학교 냉동공학과) ;
  • 김종덕 (전남대학교 생명화학공학부 생명산업공학과)
  • Published : 2009.04.29
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Abstract

Anti-angiogenic mechanism was examined for anti-obesity agents with the extract of P.radix, P.semen, S.hebra and C.furctus through anti-cell adhesion effect and western blot. Cell adhesion molecules, VCAM-1 was supressed with the order of P.radix (0.2 ppm, 125%) > P.semen (0.5 ppm, 100%) > S.hebra (5.0 ppm, 114%) > C. furctus (5.0 ppm, 111.8%), ICAM-1 was inhibited by P.radix (0.25 ppm, 130%) > P.semen (0.5 ppm, 100%) > S.hebra (5.0 ppm, 138%) > C. furctus (5.0 ppm, 66.7%), E-Selectin was also supressed P.radix (0.25 ppm, 100%) > P.semen (1.0 ppm, 128%) > S.hebra (5.0 ppm, 120%) > C. furctus (5.0 ppm, 100.7%). And signal molecules, VE-cadherin was supressed by P.radix and S.hebra, ${\beta}$-catenin was inhibited by P.radix, and Akt was supressed all these 4 kinds of natural products. These P.radix, P.semen, S.hebra and C.furctus were showed the possibility of anti-obesity agents based on anti-angiogenesis.

선별한 신생혈관형성억제제는 EGCG보다도 제어 효과가 나은 천연산물들 중에서 전호, 파고지, 희첨 및 중심으로 그 기전을 밝히고자 하였다. 세포독성은 전호는 0.3 ppm에서 약간의 독성을 나타내었고, 파고지는 10 ppm까지는 독성을 나타내지 않았으며, 희첨 및 산수유는 25 ppm까지 독성을 나타내지 않았다. 세포부착억제작용은 신생혈관의 형성에 중요한 역할을 하기 때문에 cell adhesion 분자인 VCAM-1, ICAM-1 및 E-selectin들에 대하여 ELISA 법으로 살펴보았다. VCAM-1에 대한 천연산물의 작용은 전호 (0.2 ppm, 125%)>파고지 (0.5 ppm, 100%)>희첨 (5.0 ppm, 114%)> 산수유 (5.0 ppm, 111.8%)의 순으로 저해의 강도가 높으며, ICAM-1은 전호 (0.25 ppm, 130%)>파고지 (0.5 ppm, 100%)>희첨 (5.0 ppm, 138%)>산수유 (5.0 ppm, 66.7%)의 순으로 저해되는 것으로 나타났고, 그리고 E-Selectin은 전호 (0.25 ppm, 100%)> 파고지 (1.0 ppm, 128%)>희첨 (5.0 ppm, 120%)>산수유 (5.0 ppm, 100.7%)의 순으로 저해되는 것으로 나타났다. Western blot으로부터, 전호 추출물은 VE-cadherin과 ${\beta}$-catenin의 신호전달을 억제하였으며, 그 하위 신호 전달분자인 Akt도 억제하는 것으로 나타났고, 파고지는 ${\beta}$-catenin의 신호는 억제하지 않는 것으로 보이나, 그 하위그룹의 Akt의 신호전달을 억제하는 것으로 나타났다. 그리고, 희첨은 VE-Cadherin과 하위 그룹의 Akt를 농도의 증가에 따라 확실한 제어를 보이고 있음을 알 수 있었고, 산수유는 하위그룹의 Akt의 신호전달을 억제하는 것으로 나타났다. 4종류의 선택된 천연산물은 세포표면의 신호전달분자 그리고 그 하위 그룹의 Akt를 억제함으로써 NF-kB의 활성을 차단함으로써 신생혈관의 형성을 억제하는 것으로 판단된다. 따라서 4종류의 천연산물 전호, 파고지, 희첨 및 산수유는 전반적으로 낮은 농도에서 세포부착인자의 발현을 억제하였고, 신호전달 분자들을 억제함으로써 신생혈관 형성 억제에 따른 항비만제제로서 충분한 가능성을 보였다.

Keywords

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