한국산학기술학회논문지 (Journal of the Korea Academia-Industrial cooperation Society)

  • 제10권7호
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  • Pages.1766-1772
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  • 2009
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  • 1975-4701(pISSN)
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  • 2288-4688(eISSN)
한국산학기술학회 (The Korea Academia-Industrial cooperation Society)
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한국인 우식아동으로부터 분리한 Streptococcus mutans의 내산성 단백질의 발현

Expression of Acid Stress-Induced Proteins of Streptococcus mutans Isolated from Korean Children with Caries

  • 강경희 (건양대학교 치위생학과) ;
  • 남진식 (수원여자대학 식품영양과) ;
  • 진익렬 (경북대학교 대학원 미생물학과)
  • Kang, Kyung-Hee (Department of Dental Hygiene, Konyang University) ;
  • Nam, Jin-Sik (Department of Food&Nutrition, Suwon Women's College) ;
  • Jin, Ing-Nyol (Department of Microbiology, Graduate School, Kyungpook National University)
  • 발행 : 2009.07.31
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초록

본 연구에서는 한국인 아동의 우식치아로부터 S. mutans를 분리하고, acid stress하에서 분리한 S. mutans의 내산성 능력과 관련된 단백질을 규명하고자 하였다. 2D gel electrophoresis를 수행한 결과, acid stress동안 elongation factor Ts, hypothetical protein, putative amino acid ABC transporter, adenylate kinase, fructokinase, Putative 40K cell well protein precursor, peptide deformylase, shikimate 5-dehydrogenase, mannose-6-phosphate isomerase, threonine synthase, putative dTDP-glucose-4,6-dehydratase의 발현량이 뚜렷이 증가하였으며 이들 단백질은 acid stress에 관여하는 단백질들로 추정된다.

In this study, we are interested in comparing the protein profiles of acid-shocked and control cells of S. mutans isolated from Korean children with caries. The results of 2D gel electrophoresis showed that twelve proteins are up-regulated when the cells were grown under 20 mM lactic acid stress in the exponential phase. Up-proteins under acid stress were estimated a major key of the survival and proliferation of S. mutans in low pH environments. These proteins are estimated generally associated with three biochemical pathways: glycolysis, alternative acid production and branched-chain amino acid biosynthesis.

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