Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Multiresidue Determination of Quinolones in Porcine, Chicken, and Bovine Muscle Using Liquid Chromatography with Fluorescence Detection

  • Published : 2009.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

An analytical method for the simultaneous determination of 9 quinolones (QNs) in porcine, chicken, and bovine muscles was developed and validated using liquid chromatography-fluorescence detector (LC-FLD). The samples were extracted using a liquid-liquid extraction (LLE) process. Chromatographic separation was achieved on a reverse phase $C_8$ column with a gradient elution using a mobile phase of 200 mM ammonium acetate buffer (pH 4.5) and acetonitrile (ACN). The proposed method was validated according to the Food and Drug Administration (FDA) guideline for bioanalytical assay procedures. Recoveries of QNs were 83.1-111.9% with relative standard deviations (RSDs) below 15%. Linearity within a range of 30-500 ${\mu}g/kg$ was obtained with the correlation coefficient ($R^2$) of 0.9967-0.9999. The limits of detection (LOD) were 1-16 ${\mu}g/kg$. These values were lower than the maximum residues limits (MRLs) established by the European Union (EU). The present method was successfully applied to determine QNs in edible muscles.

Keywords

References

  1. Drlica K, Zhao X. DNA gyrase, topoisomerase IV, and the 4-quinolones. Microbiol. Mol. Biol. R. 61: 377-392 (1997)
  2. Hern$\'{a}$ndez-Arteseros JA, Barbosa J, Compa$\~{n}$$\'{o}$ R, Prat MD. Analysis of quinolone residues in edible animal products. J. Chromatogr. A 945: 1-24 (2002) https://doi.org/10.1016/S0021-9673(01)01505-9
  3. Use of quinolones in food animals and potential impact on human health. Report of a WHO Meeting. Geneva, Switzerland, WHO/EMC/ZDI/98.10, http:/www.who.int/emc. Accessed Aug. 12, 2008
  4. Okerman L, Noppe H, Cornet V, De Zutter L. Microbiological detection of 10 quinolone antibiotic residues and its application to artificially contaminated poultry samples. Food. Addit. Contam. 24: 252-257 (2007) https://doi.org/10.1080/02652030600988020
  5. Commission Regulation (EC) 1181/2002, Amending Annexes I of Council Regulation (EEC) no. 2377/90 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin (text, with EEA relevance). Off. J. Eur. Commun. L221: 8-36 (2002)
  6. Watanabe H, Satake A, Kido Y, Tsuji A. Monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic assay for enrofloxacin in biological matrices. Analyst 127: 98-103 (2002) https://doi.org/10.1039/b109427k
  7. Christodoulou EA, Samanidou VF, Papadoyannis IN. Validation of an HPLC-UV method according to the European Union Decision 2002/657/EC for the simultaneous determination of 10 quinolones in chicken muscle and egg yolk. J. Chromatogr. B 859: 246-255 (2007) https://doi.org/10.1016/j.jchromb.2007.10.009
  8. Bailac S, Ballesteros O, Jim$\'{e}$nez-Lozano E, Barr$\'{o}$n D, Sanz-Nebota V, Naval$\'{o}$n A, V$\'{\i}$lchez JL, Barbosa J. Determination of quinolones in chicken tissues by liquid chromatography with ultraviolet absorbance detection. J. Chromatogr. A 1029: 145-151 (2004) https://doi.org/10.1016/j.chroma.2003.11.110
  9. Zhao S, Jiang H, Li X, Mi T, Li C, Shen J. Simultaneous determination of trace levels of 10 quinolones in swine, chicken, and shrimp muscle tissues using HPLC with programmable fluorescence detection. J. Agr. Food. Chem. 55: 3829-3834 (2007) https://doi.org/10.1021/jf0635309
  10. Verdon E, Couedor P, Roudaut B, Sand$\'{e}$rs P. Multiresidue method for simultaneous determination of ten quinolone antibacterial residues in multimatrix/multispecies animal tissues by liquid chromatography with fluorescence detection: Single laboratory validation study. J. AOAC. Int. 88: 1179-1192 (2005)
  11. Idowu OR, Peggins JO. Simple, rapid determination of enrofloxacin and ciprofloxacin in bovine milk and plasma by high-performance liquid chromatography with fluorescence detection. J. Pharm. Biomed. Anal. 35: 143-153 (2004) https://doi.org/10.1016/j.jpba.2004.01.006
  12. Chu PS, Wang RC, Chu HV. Liquid chromatographic determination of fluoroquinolones in egg albumen and egg yolk of laying hens using fluorometric detection. J. Agr. Food. Chem. 50: 4452-4455 (2002) https://doi.org/10.1021/jf0202032
  13. Schneider MJ, Donghue DJ. Multiresidue analysis of fluoroquinolone antibiotics in chicken tissue using liquid chromatographyfluorescence-multiple mass spectrometry. J. Chromatogr. B 780: 83-92 (2002) https://doi.org/10.1016/S1570-0232(02)00437-3
  14. Bailac S, B$\'{a}$rron D, Barbosa J. New extraction procedure to improve the determination of quinolones in poultry muscle by liquid chromatography with ultraviolet and mass spectrometric detection. Anal. Chim. Acta 580: 163-169 (2006) https://doi.org/10.1016/j.aca.2006.07.064
  15. Schneider MJ, Reyes-Herrera I, Donoghue DJ. Evaluation of serum as a potential matrix for multiresidue determination of fluoroquinolone antibiotics in chicken using liquid chromatography-fluorescencemass spectrometry(n). J. AOAC. Int. 90: 1716-1723 (2007)
  16. Yorke JC, Froc P. Quantitation of nine quinolones in chicken tissues by high-performance liquid chromatography with fluorescence detection. J. Chromatogr. A 882: 63-77 (2000) https://doi.org/10.1016/S0021-9673(00)00165-5
  17. Christodoulou EA, Samanidou VF, Papadoyannis IN. Development and validation of an HPLC confirmatory method for residue analysis of ten quinolones in tissues of various food-producing animals, according to the European Union Decision 2002/657/EC. J. Sep. Sci. 30: 2676-2686 (2007) https://doi.org/10.1002/jssc.200700170
  18. Jimenez-Lozano E, Roy D, Barron D, Barbosa J. Effective sorbents for solid-phase extraction in the analysis of quinolones in animal tissues by capillary electrophoresis. Electrophoresis 25: 65-73 (2004) https://doi.org/10.1002/elps.200305656
  19. US Department of Health and Human Services, Food and Drug Administration, Center Drug Evaluation and Research (CDER), Center for Veterinary Medicine (CVM): Guidance for Industry, Bioanalytical Method. Available from: http://www.fda.gov/cder/guidance/index.htm. Accessed Jun. 20, 2008
  20. ICH. Guidance for Industry, Q2B Validation of Analytical Procedures: Methodology. ICH-Q2B (1996)
  21. Clemente M, Hermo MP, B$\'{a}$rron D, Barbosa J. Confirmatory and quantitative analysis using experimental design for the extraction and liquid chromatography-UV, liquid chromatography-mass spectrometry, and liquid chromatography-mass spectrometry/mass pectrometry determination of quinolones in turkey muscle. J. Chromatogr. A 1135: 170-178 (2006) https://doi.org/10.1016/j.chroma.2006.09.041
  22. Christodoulou EA, Samanidou VF, Papadoyannis IN. Development of an HPLC multi-residue method for the determination of ten quinolones in bovine liver and porcine kidney according to the European Union Decision 2002/657/EC. J. Sep. Sci. 31: 119-127 (2008) https://doi.org/10.1002/jssc.200700297
  23. Choi HJ, Abd El-Aty AM, Goudah A, Sung GM, Yi H, Seo DC, Kim JS, Shim JH, Jeong JY, Lee SH, Shin HC. Monitoring of fluoroquinolone residual levels in chicken eggs by microbiological assay and confirmation by liquid chromatography. Biomed. Chromatogr. 22: 92-99 (2008) https://doi.org/10.1002/bmc.900