DOI QR코드

DOI QR Code

Occurrence of Stem Canker on Rape Caused by Leptosphaeria biglobosa in Korea

  • Hong, Sung-Kee (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Shin, Dong-Beom (Crop Environment Research Division, National Institute of Crop Science, RDA) ;
  • Choi, Hyo-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lee, Young-Kee (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lee, Sang-Yeob (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA))
  • Published : 2009.09.30

Abstract

Stem canker symptoms were observed in a rape field in Muan, Korea during a disease survey in May 2006. A total of 15 isolates of Phoma sp. were obtained from the infected stems of the plant. All isolates were identified as Leptosphaeria biglobosa based on their morphological and cultural characteristics. The Korean isolates of L. biglobosa were assigned to 'brassicae' among six subclades of L. biglobosa complex based on the entire ITS sequences of rDNA. Pathogenicity of the fungal isolates was confirmed on leaves and stems of rape by artificial inoculation. This is the first report that Leptosphaeria biglobosa causes stem canker of rape in Korea.

Keywords

References

  1. Balesdent, M. H., Gall, C., Robin, P. and Rouxel, T. 1992. Intraspecific variation in soluble mycelial protein and esterase patterns of Leptosphaeria maculans French isolates. Mycol. Res. 96:677-684 https://doi.org/10.1016/S0953-7562(09)80497-8
  2. Balesdent, M. H., Jedryczka, M., Jain, L., Mendes-Pereira, E., Bertrandy, J. and Rouxel, T. 1998. Conidia as a substrate for internal transcribed spacer-based PCR identification of members of the Leptosphaeria maculans species complex. Phytopathology 88:1210-1217 https://doi.org/10.1094/PHYTO.1998.88.11.1210
  3. Boerema G. H., Gruyter, J., Noordeloos, M. E. and Hamers, M. E. C. 2004. Phoma identification manual. Differentiation of specific and intra-specific taxa in culture. CAB International, Wallingford, UK. 448 pp
  4. Cho, W. D. and Shin, H. D. eds. 2004. List of plant diseases in Korea. 4th edition. The Korean Society of Plant Pathology. Suwon, Korea. 779 pp
  5. Fitt, B., Brun, H., Barbetti, M. and Rimmer, S. 2006. World-wide importance of phoma stem canker (Leptosphaeria maculans and L. biglobosa) on oilseed rape (Brassica napus). Eur. J. Plant Pathol. 114:3-15 https://doi.org/10.1007/s10658-005-2233-5
  6. Gall, C., Balesdent, M. H., Desthieux, I., Robin, P. and Rouxel, T. 1995. Polymorphism of $Tox^{0}$ Leptosphaeria maculans isolates as revealed by soluble protein and isozyme electrophoresis. Mycol. Res. 99:221-229 https://doi.org/10.1016/S0953-7562(09)80889-7
  7. Jedryczka, M., Rouxel, T. and Balesdent, M. H. 1999. Rep-PCR. based genomic fingerprinting of isolates of Leptosphaeria. maculans from Poland. Eur. J. Plant Pathol. 105:813-823 https://doi.org/10.1023/A:1008713907780
  8. Koch, E., Song, K., Osborn, T. C. and Williams, P. H. 1991. Relationship between pathogenicity. and phylogeny based on restriction fragment length polymorphism in Leptosphaeria maculans. Mol. Plant-Microbe Intract. 4:341-349 https://doi.org/10.1094/MPMI-4-341
  9. Mendes-Pereira, E., Balesdent, M. H., Brun, H. and Rouxel, T. 2003. Molecular phylogeny of the Leptosphaeria maculans-L. biglobosa species complex. Mycol. Res. 107:1287-1304 https://doi.org/10.1017/S0953756203008554
  10. Shoemaker, R. A. and Brun, H. 2001. The teleomorph of the weakly aggressive segregate of Leptosphaeria maculans. Can. J. Bot. 79:412-419 https://doi.org/10.1139/cjb-79-4-412
  11. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. BioI. Evol. 24:1596-1599 https://doi.org/10.1093/molbev/msm092
  12. Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic. Acids Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  13. Vincenot, L., Balesdent, M. H., Li, H., Barbetti, M. J., Sivasithamparam, K., Gout, L. and Rouxel, T. 2008. Occurrence of a new subclade of Leptosphaeria biglobosa in Western Australia. Phytopathology 98:321-329 https://doi.org/10.1094/PHYTO-98-3-0321
  14. Voigt, K., Jedryczka, M. and Wostemeyer, J. 2001. Strain typing of Polish Leptosphaeria maculans isolates supports at the genonomic level the multispecies concept of aggressive and non-agressive strains. Microbial. Res. 156:169-177 https://doi.org/10.1078/0944-5013-00099
  15. West, J. S., Kharbanda, P. D., Barbetti, M. J. and Fitt, B. D. L. 2001. Epidemiology and management of Leptosphaeria maculans (Phoma stem. canker) on oilseed rape in Australia, Canada and Europe. Plant Pathol. 50:10-27 https://doi.org/10.1046/j.1365-3059.2001.00546
  16. White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications. Academic press, New York, USA
  17. Williams R. H. and Fitt, B. D. L. 1999. Differentiating A and B groups of. Leptosphaeria maculans, causal agent of stem canker (blackleg) of. oilseed rape. Plant Pathol. 48:161-175 https://doi.org/10.1046/j.1365-3059.1999.00333.x

Cited by

  1. Phoma leaf spot of wasabi (Wasabia japonica) caused byLeptosphaeria biglobosa vol.66, pp.3, 2017, https://doi.org/10.1111/ppa.12589
  2. Specific and Sensitive Detection of Phoma glomerata Using PCR Techniques vol.41, pp.1, 2013, https://doi.org/10.4489/KJM.2013.41.1.52
  3. Korean Brassica oleracea germplasm offers a novel source of qualitative resistance to blackleg disease 2017, https://doi.org/10.1007/s10658-017-1210-0