대한본초학회지 (The Korea Journal of Herbology)

  • 제24권3호
  • /
  • Pages.103-108
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  • 2009
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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포공영 추출물의 항산화 효과 및 피부 각질세포 보호효과

Anti-oxidative Effects of Taraxaci Herba and Protective Effects on Human HaCaT Keratinocyte

  • 김형우 (부산대학교 한의학전문대학원) ;
  • 김병주 (부산대학교 한의학전문대학원) ;
  • 임세현 (고려대학교 간호대학) ;
  • 김현영 (동의대학교 한의과대학) ;
  • 이숙영 (조선대학교 치과대학) ;
  • 조수인 (부산대학교 한의학전문대학원) ;
  • 김영균 (동의대학교 한의과대학)
  • 발행 : 2009.09.30
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초록

Objectives : This study was carried out to investigate anti-oxidative effects of Taraxaci Herba (TH) and protective effects on Human HaCaT keratinocyte. Methods : Anti-oxidative effects were measured by estimating the amount of total phenolics and flavonoids. In addition, DPPH free radical scavenging activities were estimated. Protective effects of TH on HaCaT keratinocytes against oxidative stress induced by hydrogen peroxide were also measured. Results : In our results, treatment with TH did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 200 $\mu$g/ml. 42.64$\pm$1.90 $\mu$g/ml of total phenolics and 28.09$\pm$1.84 $\mu$g/ml of flavonoids was detected from TH ethanol extract. In addition, DPPH free radical scavenging activities of TH were elevated in dose-dependent manner. In addition, The value of half maximal inhibitory concentration (IC$_{50}$) was 165.5 $\mu$g/ml. Finally, TH showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly. Conclusions : In conclusion, these results suggest that TH may have anti-oxidantic action in human skin and also suggest the possibility as cosmetic material.

키워드

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