대한본초학회지 (The Korea Journal of Herbology)

  • 제24권3호
  • /
  • Pages.79-86
  • /
  • 2009
  • /
  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

황련의 항염증효과

Anti-inflammatory Effect of Coptidis Rhizoma

  • 윤광로 (상지대학교 보건과학대학) ;
  • 김영진 (상지대학교 한의과대학) ;
  • 이은 (상지대학교 보건과학대학) ;
  • 이준무 (상지대학교 한의과대학)
  • Yoon, Kwang-Ro (College of Health Sciences, Sangji University) ;
  • Kim, Young-Jin (College of Oriental Medicine, Sangji University) ;
  • Lee, Eun (College of Health Sciences, Sangji University) ;
  • Lee, Joon-Moo (College of Oriental Medicine, Sangji University)
  • 발행 : 2009.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : The present study investigated Inflammatory effect of Coptidis Rhizoma in lipopolysaccharideexposed rats and Raw 264.7 cells. Methods： The plasma concentration of IL-1$\beta$, IL-6 and TNF-$\alpha$ peaked at 5 h after LPS injection, and the values of the Coptidis Rhizoma extract groups were lower than those of the control group. In the increment of cytokines concentration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were lower than that of control group. The plasma concentration of IL-10 peaked at 5 h after LPS injection, and the values of the Coptidis Rhizoma extract groups were higher than those of the control group. In the increment of cytokines concentration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were higher than that of control group. Liver cytokines measurement was done at 5 h after LPS injection. The concentration of liver IL-1$\beta$ and IL-6 in the Coptidis Rhizoma groups was lower than that of the control group. The concentrations of liver TNF-$\alpha$, and IL-10 showed no significant differences among all the treatment groups. Results： In the studies of lipopolysaccharide-exposed Raw 264.7 cells, the concentration of IL-1$\beta$, IL-6 and TNF-$\alpha$ in the lipopolysaccharide-exposed cells groups was higher than that of control group (normal group), and in the lipopolysaccharide-exposed cells groups, these values showed a tendency to decrease in the Coptidis Rhizoma groups. The concentration of IL-10 in the lipopolysaccharide-exposed cells groups was higher than that of control group (normal group), and in the lipopolysaccharide-exposed cells groups, the values showed a tendency to increase in the Coptidis Rhizoma groups. Conclusions： These results indicate that the Coptidis Rhizoma extracts have an functional material for Inflammatory activities.

키워드

참고문헌

  1. Rabson A, IM Roitt, PJ Delves. Really Essential Medical Immunology. Oxford : Blackwell publishing Ltd. 2005 ; 1-14.
  2. Chao CY, SL Yeh, MT Lin, WJ Chen. Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids, and inflammatory mediators in septic rats. Nutrition. 2000 ; 16 : 284-8. https://doi.org/10.1016/S0899-9007(99)00299-3
  3. Boumpas DT, GP Chrousos, RL Wilder. Glucocorticoid therapy for immune mediated disease: Casic and clinical correlates. Ann Intern Med. 1993 ; 119 : 1198-208. https://doi.org/10.7326/0003-4819-119-12-199312150-00007
  4. Barton CC, EX barton, PE Ganey, SL Kunkel, RA Roth. Bacterial lipopolysaccharide enhances aflatoxin B1 hepatotoxicity in rats by a mechanism that depends on tumor necrosis factor-$\alpha$. Hepatology. 2001 ; 33 : 66-73. https://doi.org/10.1053/jhep.2001.20643
  5. Surh YJ. Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem Toxicol. 2002 : 40(8) : 1091-7. https://doi.org/10.1016/S0278-6915(02)00037-6
  6. Seo WG, Pae HO, Oh GS, KY Chai, Kwon TO, Yun YG, Kim NY, Chung HT. Inhibitory rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, Raw 264.7 cells. J Ethnopharmacol. 2001 ; 76 : 59-64. https://doi.org/10.1016/S0378-8741(01)00221-5
  7. 김태희, 양기숙, 황은진, 박성배. 마황의 면역작용에 미치는 영향. 생약학회지. 1991 ; 22(3) : 183-91.
  8. Lee E. Anti-inflammatory effect of Scutellariae Radix. Korean J. Plant Res. 2007 ; 20(6) : 548-22.
  9. 남정연, 이현선, 이승웅, 정미연, 최정호, 유은숙, 노문철, 김영국. 감국에서 분리한 Kikkanol F Monoacetate와 5-Hydroxy-6,7,3',4'-tetramethoxyflavone의 IL-6생성억제활성. 생약학회지. 2005 ; 36(3) : 186-90.
  10. 김선재, 박윤미, 정순택. 감국추출물의 항충치효과와 Glucosyltransferase 저해활성탐색. Korean food culture. 2005 ; 20(3) : 341-5.
  11. 고인자, 유승조, 이은방. 한국산 하고초류의 약물학적 연구(1). 생약학회지. 1986 ; 17(3) : 232-41.
  12. Lee DU, Kang YJ, Park MK, Lee YS, Seo HG, Kim TS, Kim CH, Chang KC. Effects of 13-alkylsubstituted berberine alkaloids on the expression of COX-II, TNF-alpha, iNOS, and IL-12 production in LPS-stimulated macrophages. Life Science. 2003 ; 73 : 1401-12. https://doi.org/10.1016/S0024-3205(03)00435-1
  13. Kim EK, Kwon KB, Han MJ, Song MY, Lee JH, Lv N, Ka SO, Yeom SR, Kwon YD, Ryu DG, Kim KS, Park JW, Park R, Park BH. Coptidis rhizoma extract protects against cytokine-induced death of pancreatic beta-cells through suppression of NKkappaB activation. Exp Mol Med. 2007 ; 39 : 149-59. https://doi.org/10.1038/emm.2007.17
  14. Enk R, Ehehalt R, Graham JE, Bierhaus A, Remppis A, Greten HJ. Differential effect of Rhizoma coptidis and its main alkaloid compound berberine on TNF-alpha induced NFkappaB translocation in human keratinocytes. J Ethnopharmacol. 2007 ; 109 : 170-5. https://doi.org/10.1016/j.jep.2006.07.013
  15. Wang X, Xing D, Wang W, Su H, Tao J, Du L. Pharmacokinetics of berberine in rat thalamus after intravenous administration of Coptidis rhizoma extract. Am J Chin Med. 2005 ; 33 : 935-43. https://doi.org/10.1142/S0192415X05003557
  16. Marriot JB, M Westby, S Cookson, M Guckian, S Goodbourn, G Muller. CC-3052: a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-$\alpha$ production. J Immunol. 1998 ; 161 : 4236-43.
  17. Mathiak G, G Grass, T Herzmann, T Luebke, C Cu-Zetina, SA Boehm. Capase-1-inhibitor ac-YVADcmk reduces LPS-lethality in rats without affecting haematology or cytokine responses. Br J Pharmacol. 2000 ; 131 : 383-6. https://doi.org/10.1038/sj.bjp.0703629
  18. Eduard FM, Martha SMR, Victor PA, Pablo M. Immunomodulatory effects of thalidomide analogs on LPS-induced plasma and hepatic cytokines in the rat. Biochemical pharmacology. 2004 ; 68 : 1321-9. https://doi.org/10.1016/j.bcp.2004.06.018
  19. Aono K, K Isobe, K Kuichi, Z Fan, M Ito, A Takeuchi. In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines. J cell Biochem. 1997 ; 65 : 349-58. https://doi.org/10.1002/(SICI)1097-4644(19970601)65:3<349::AID-JCB5>3.0.CO;2-S
  20. Corral LG, GW Muller, AL Moreira, X Chen, M Wu, D Stirling. Selection of novel analogs of thalidomide with enhanced tumor necrosis factor-$\alpha$ inhibitory activity. Mol med. 1996 ; 25 : 964-9.
  21. Sang H, GL Wallis, CA Stewart, K Yashige. Expression of cytokines and activation of transcription factors in lipopolysaccharide-administered rats and their inhibition by phenyl N-tert-butylnitrone (PBN). Arch Biochem Biophys. 1999 ; 363 : 341-8. https://doi.org/10.1006/abbi.1998.1086
  22. Harry D, R Anand, S Holt, S Davies, R Marley, B Fernando. Increased sensitivity to endotoxemia in the bile duct-ligated cirrhotic rat. Hepatology. 1999 ; 30 : 1198-205. https://doi.org/10.1002/hep.510300515
  23. Chamulitrat W, ME Blazka, SJ Jordan, MI Luster, RP Mason. Tumor necrosis factor-$\alpha$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 1995 ; 24 : 2273-80. https://doi.org/10.1016/0024-3205(79)90104-8
  24. Harbrecht BG, M DiSilvio, AJ Demetris, RL Simmons, TR Billiar. Tumor necrosis factor-$\alpha$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology. 1994 ; 20 : 1055-60. https://doi.org/10.1002/hep.1840200439
  25. Hamada E, T Nishida, Y Uchiyama, J Nakamura, K Isihara, H Kazuo. Activation of Kupffer cells and caspases-3 involved in rat hepatocyte apoptosis induced by endotoxin. J Hepatol. 1999 ; 30 : 807-18. https://doi.org/10.1016/S0168-8278(99)80133-0
  26. Thompson KC, A Trowern, A Fowell, M Marathe, C Haycock, MJP Arthur et al. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology. 1998 ; 28 : 1518-24. https://doi.org/10.1002/hep.510280611
  27. Moreira AL, EP Sampaio, A Zmuidzinas, P Frindt, KA Smith, G Kaplan. Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation. J Exp Med. 1993 ; 177 : 1675-80. https://doi.org/10.1084/jem.177.6.1675
  28. Simpson KJ, Lukacs NW, Colletti L, Strieter RM, Kunkel SL. Cytokines and the liver. J Hepatol. 1997 ; 27 : 1120-32. https://doi.org/10.1016/S0168-8278(97)80160-2
  29. Klapproth J, Geiger T, Andus T, Heirich PC. Fate and biological action of human recombinant interleukin 1$\beta$ in the rat in vivo. Eur J Immunol. 1980 ; 19 : 1485-90.
  30. Louis H, LeMoine O, Peny MO, Quertinmont E, Fokan D, Goldman M et al. Production and role of interleukin-10 in concanavalin A-induced hepatitis in mice. Hepatology. 1997 ; 25 : 1382-9. https://doi.org/10.1002/hep.510250614