Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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MicroRNA Expression Profiles in Korean Non-Small Cell Lung Cancer

  • Son, Ji Woong (Department of Internal Medicine, Konyang University Hospital) ;
  • Kim, Young Jin (Department of Thoracic Surgery, Konyang University Hospital) ;
  • Cho, Hyun Min (Department of Thoracic Surgery, Konyang University Hospital) ;
  • Lee, Soo Young (Myunggok Research Institute for Medical Science, Konyang University) ;
  • Jang, Jin Sung (Department of Biochemistry, Kyungpook National University School of Medicine) ;
  • Choi, Jin Eun (Department of Biochemistry, Kyungpook National University School of Medicine) ;
  • Lee, Jung Uee (Department of Hospital Pathology, Daejeon St. Mary's Hospital) ;
  • Kang, Min Gyu (Department of Internal Medicine, Konyang University Hospital) ;
  • Lee, Yu Mi (Department of Internal Medicine, Konyang University Hospital) ;
  • Kwon, Sun Jung (Department of Internal Medicine, Konyang University Hospital) ;
  • Choi, Eugene (Department of Internal Medicine, Konyang University Hospital) ;
  • Na, Moon Jun (Department of Internal Medicine, Konyang University Hospital) ;
  • Park, Jae Yong (Department of Biochemistry, Kyungpook National University School of Medicine)
  • Received : 2009.08.14
  • Accepted : 2009.09.18
  • Published : 2009.11.30
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Abstract

Background: MicroRNAs (miRNAs) play an important role in the regulation of cell proliferation, apoptosis, development and differentiation. Several studies have shown that aberrant expression of miRNAs is involved in cancer development and progression by regulating the expression of proto-oncogenes or tumor suppressor genes. In this study, we investigated miRNA expression profiles in Korean patients with non-small cell lung cancer (NSCLC). Methods: We performed miRNA microarray analysis containing 60~65 bp oligonucleotide probes representing human 318 miRNAs and validated the results of the microarray with Northern blot analysis or quantitative RT-PCR. Next, we examined the correlation between miRNA expression and the target gene transcriptional profile using a human whole-genome-expression microarray. Results: We showed that 35 miRNAs were expressed differentially in the NSCLCs and corresponding non-malignant lung tissues. We showed that 35 miRNAs were expressed differentially in the NSCLCs and corresponding nonmalignant lung tissues. Thirteen of the 35 differentially expressed miRNAs were newly identified in the present study. Of the 35 miRNAs, 2 (miR-371 and miR-210) were over-expressed in lung cancers, and 33 miRNAs, including miR-145, were under-expressed in lung cancers. miR-99b expression consistently showed a negative correlation with FGFR3 expression. Conclusion: Albeit a small number of patients were examined, these results suggest that miRNA expression profiles in Korean lung cancers may be somewhat different from the expression profiles reported on lung cancers in Western populations. The findings suggest that miR-99b might be a tumor suppressor through its up-regulation of FGFR3.

Keywords

References

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