Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antimicrobial Property of honeybee (Apis mellifera L.) venom against Propionibacterium acnes and Aerobic Skin Flora

국내산 봉독의 여드름 유발균 및 피부 상재균 증식 억제 효과

  • Han, Sang-Mi (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Kwang-Gil (National Academy of Agricultural Science, Rural Development Administration) ;
  • Yeo, Joo-Hong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Wan-Tae (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Kwan-Kyu (College of Medicine, Catholic University of Daegu)
  • 한상미 (농촌진흥청 국립농업과학원) ;
  • 이광길 (농촌진흥청 국립농업과학원) ;
  • 여주홍 (농촌진흥청 국립농업과학원) ;
  • 김원태 (농촌진흥청 국립농업과학원) ;
  • 박관규 (대구가톨릭대학과 의과대학)
  • Published : 2009.09.30
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Abstract

The in vitro antibacterial activities of honeybee(Apis mellifera. L) venom collected by a bee venom collector were investigated against several bacteria including antibiotic-susceptible and resistant Propionibacterium acnes. Honeybee venom was prepared with different concentrations and they showed strong antibacterial activites. Honeybee venom inhibited the growth of the tested antibiotic-resistant P. acnes at the concentration of 1 mg/ml. The inhibitory activities of the honeybee venom showed time-dependent manner. Honeybee venom did not influence the viability of human dermal fibroblast at the high concentration of less than 10 mg/ml. From these results, we expect that honeybee venom has strong antibacterial activities and has advantage for treating cure.

Keywords

References

  1. Van de kerkhof, P. C., Kleinpenning, M. M., de Jong, E. M., van Dooren-Greebe, R. J. and Alkemade, H. A. (2006) Current and future treatment options for acne. J Dermatolog Treat 17(4): 198-204 https://doi.org/10.1080/09546630600830596
  2. Freak, J. (2006) An overview of acne vulgaris. Nurs Times 102(30): 30-32
  3. Taub, A. F. (2007) Procedural treatments for acne vulgaris. Dermatol Surg 33(9): 1005-1026 https://doi.org/10.1111/j.1524-4725.2007.33215.x
  4. 임연순, 명기범, 정낙은, 정화순 (1995) 여드름 환자에서 Propionibacterium acnes에 대한 항생제의 최저발육저지농도에 관한 연구. 대한피부과학회지 33(3): 437-444
  5. 유종엽, 박서형, 황인아, 조성진, 허창훈, 윤상웅, 박경찬,(2003) 상백피 추출물과 차나무 기름 제제가 여드름과 호기성 피부 상재균에 미치는 효과. 대한피부과학회지 41(9):1136-1141
  6. 이두락, 박미연 (2007) Indocyanine Green과 805 nm Diode Laser를 이용한 여드름 치료에 대한 유효성 평가. 대한피부과학회지 46(9): 1208-1215
  7. Nakatsuji, T., Kao, M. C., Fang, J. Y., Zouboulis, C. C., Zhang, L., Gallo, R.L. and Huang, C. M. (2009) Animicrobial Property of Lauric Acid Against Propionibacterium Acnes: Its Therapeutic Potential for Inflammatory Acne Vulgaris. J Invest Dermatol Epub in press https://doi.org/10.1038/jid.2009.93.
  8. Heller, S., Kellenberger, L. and Shapiro, O. (2007) Antipropionibacterial Activity of BAL19403, a Novel Macrolide Antibiotic. Antimicrob Agents Chemother 51(9): 1956-1961
  9. Jacqueline, M. B. and Susan, M. P. (1983) Resistance of Propionibacteria to antibiotics used in the treatment of acne. J Med Microbiol 16: 271-280 https://doi.org/10.1099/00222615-16-3-271
  10. 석귀남, 이성하, 김경신 (2004) 새삼 (Cuscuta japonica Choisy) 및 실세삼 (C. australis R.Be) 추출물의 여드름 유발균 Propionibacterium acnes 증식 억제 효과. 생약학회지 35(4): 375-379
  11. 김민정, 박상동, 이아람, 김경호, 장준혁, 김갑성 (2002) 쥐의 Collagen 유발 관절염의 활액에서 단백분해효소의 활성 및 유리기 손상에 미치는 봉독약침의 억제효과. 대한침구학회지 19(5): 161-175
  12. Kim, H. W., Kwon, Y. B., Ham, T. W., Roh, D. H., Yoon, S. Y., Lee, H. J., Han, H. J., Yang, I. S., Beitz, A. J. and Lee, J. H. (2003) Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord fos expression in rats. J Vet Med Sci 65(3):349-355 https://doi.org/10.1292/jvms.65.349
  13. Habermann, E. and Reiz, K. G. (1965) On the biochemistry of bee venom pep-tides, melittin and apamin. Bioche-mistry 343:192-203
  14. James, A. V., Glenn, B. W. and Robert, B. B. (1975-1976) The effect of treatment with whole bee venom on cage activity and plasma cortisol levels in the arthritic dog. Inflammation 1(2): 167-174 https://doi.org/10.1007/BF00917527
  15. Piek, T. (1986) Venoms of the Hymenoptera. Academic Press. London
  16. Curcio-Vonlanthen, V., Schneider, C. H., Frutig, K., Blaser, K. and Kalbacher, H. (1997). Molecular parameters in melittin immunogenicity. J Pept Sci 3(4):267-276 https://doi.org/10.1002/(SICI)1099-1387(199707)3:4<267::AID-PSC106>3.0.CO;2-7
  17. Rudenko, S. V. and Nipot, E. E. (1996) Modulation of melittin-induced hemolysis of erythocytes. Biokhimiia 61(12):2116-2124
  18. Han, S. M., Lee, K. G., Yeo, J. H., Kweon, H. Y., Woo, S. O.,Baek, H. J. and Park, K. K. (2007) Inhibitory effect of bee venom against ultraviolet B induced MMP-1 and MMP-3 in human dermal fibroblasts. J Apic Res 46(2): 94-98
  19. Fennell, J. F., Shipman, W. H. and Cole, L. J. (1967) Antibacterial action of a bee venom fraction (melittin) against a penicillin-resistant staphylococcus and other microorganisms. Res Dev Tech Rep 5:1-13
  20. 한상미, 이광길, 여주홍, 권해용, 이인경, 이명렬, 이만영, 백하주, 배기환 (2005) 전기충격법으로 채취한 국내산 봉독의 항균효과 (I). 한국양봉학회지 20(1); 53-58
  21. Wu, M. and Hancock, R. E. (1999) Interaction of the cyclic antimicrobial cationic peptide bactenecin with the outer and cytoplasmic membrane. J Biol Chem 274(1): 29-35 https://doi.org/10.1074/jbc.274.1.29
  22. 김나래, 임영희, 박설웅, 남은실, (2009) 천연물유래 여드름치료제제의 향균성 측정. Kor. J. Microbiol. Biotechnol 37(1): 80-84