생명과학회지 (Journal of Life Science)

  • 제19권9호
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  • Pages.1294-1298
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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대장암 세포주에서 genistein과 daidzein의 병합처리에 의한 상승적인 세포독성 효과

Synergistic Cytotoxic Effects by Combination Treatment of Genistein and Daidzein in Human Colorectal Cancer Cell

  • 손성민 (안동대학교 자연과학대학 생명과학과) ;
  • 임승현 (안동대학교 자연과학대학 생명과학과) ;
  • 김효림 (안동대학교 자연과학대학 생명과학과) ;
  • 정민정 (안동대학교 자연과학대학 생명과학과) ;
  • 김태완 (안동대학교 자연과학대학 식품생명공학과) ;
  • 이종화 (안동대학교 자연과학대학 식품생명공학과) ;
  • 김종식 (안동대학교 자연과학대학 생명과학과)
  • Son, Seong-Min (Dept. of Biological Sciences, Andong National University) ;
  • Lim, Seung-Hyun (Dept. of Biological Sciences, Andong National University) ;
  • Kim, Hyo-Rim (Dept. of Biological Sciences, Andong National University) ;
  • Kim, Min-Jeong (Dept. of Biological Sciences, Andong National University) ;
  • Kim, Tae-Wan (Dept. of Food Science and Biotechnology, Andong National University) ;
  • Lee, Jong-Hwa (Dept. of Food Science and Biotechnology, Andong National University) ;
  • Kim, Jong-Sik (Dept. of Biological Sciences, Andong National University)
  • 발행 : 2009.09.30
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초록

콩의 대표적인 이소플라본인 genistein과 daidzein에 의해 암세포 생존율에 미치는 영향을 확인하기 위하여, HCT116 세포주에 genistein과 daidzein을 농도 의존적으로 처리하였다. Genistein은 처리한 농도 의존적으로 암세포 생존율을 감소시켰으며, 이에 반해 daidzein은 세포생존율에 큰 변화를 보여주지는 못하였다. 이전의 마이크로어레이 실험 결과에 의하면, $50{\mu}M$의 genistein에 의해 2배 이상 증가되는 유전자 71개, 2배 이상 감소되는 유전자 64개가 검색되었다. 이중 3개의 유전자(DKK-1, ATF3 그리 고 NAG-1)를 선택하여, 마이크로어레이 실험 결과를 검증하기 위하여 RT-PCR을 수행하였다. RT-PCR 결과 마이크로어레이 결과와 모두 일치함을 증명하였다. 한편, genistein과 daidzein에 의한 병합처리에 의해 암세포생존에 미치는 영향을 확인하였다. 그 결과 병합처리에 의한 상승적인 세포독성 효과를 확인하였다. RT-PCR과 real-time PCR의 결과 genistein과 daidzein의 병합처리에 의해 항암유전자인 NAG-1 유전자가 상승적으로 발현이 증가됨을 확인하였다. 이러한 결과는 이소플라본뿐만 아니라 대두제품에 의한 암 화학예방법의 기전을 이해하는 도움을 줄 것으로 생각된다.

To investigate whether isoflavone genistein and daidzein could affect cancer cell viability, human colorectal HCT116 cells were incubated with genistein or daidzein in a dose-dependent manner. Genistein decreased cancer cell viability in a dose-dependent manner, whereas daidzein did not show dramatic cytotoxic effects. We also found that 71 genes were up-regulated more than 2-fold, whereas 64 genes were down-regulated more than 2-fold with 24 hr of $50{\mu}M$ genistein treatment by our previous microarray data. Among the up-regulated genes, we selected 3 genes (DKK1, ATF3 and NAG-1) and performed RT-PCR to confirm microarray data. The results of RT-PCR were highly correlated with those of the microarray experiment. In addition, we investigated whether a combination treatment of genistein and daidzein could affect cancer cell viability. Surprisingly, the combination treatment did show synergistic cytotoxic effects detected by MTS assay. The results of RT-PCR and real-time PCR indicate that a combination of genistein and daidzein can synergistically induce NAG-1 expression in HCT116 cells. This result implies that NAG-1 induction is highly associated with synergistic cytotoxic effects induced by a combination treatment of genistein and daidzein. Overall, these results may provide a clue in explaining the anti-cancer activity of soy bean in human colorectal cancer.

키워드

참고문헌

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  1. Anticancer Activity of Olive Oil Hydroxytyrosyl Acetate in Human Adenocarcinoma Caco-2 Cells vol.61, pp.13, 2013, https://doi.org/10.1021/jf305158q