Changes of Saponin Contents of Leaves, Stems and Flower-buds of Panax ginseng C. A. Meyer by Harvesting Days

인삼 지상부의 채취시기에 따른 사포닌조성 비교

  • Choi, Jae-Eul (College of Agric. and Life Science, Chungnam National University) ;
  • Li, Xiangguo (College of Agric. and Life Science, Chungnam National University) ;
  • Han, Young-Hwan (WaterBio co. ltd) ;
  • Lee, Ki-Teak (College of Agric. and Life Science, Chungnam National University)
  • 최재을 (충남대학교 농업생명과학대학) ;
  • 이상국 (충남대학교 농업생명과학대학) ;
  • 한용환 ((주)워터바이오) ;
  • 이기택 (충남대학교 농업생명과학대학)
  • Published : 2009.08.30

Abstract

The parts of leaves, flowers and stems in ginseng were obtained for analyzing the component of saponin on 15th April, 25th April, 5th May, 25th May, which were considered as ginseng foliation stage. The total saponin content of the leaves were 97.29, 66.42, 67.61, 36.24 mg/g, respectively, in which the content of Re, $Rb_1$ and Rd were more than 2/3 amount of total saponin. Especially, the saponin content of leaves decreased according to the sequential collection days, in which the similar results were observed from the flowers and stems of ginseng. The total saponin content of the flowers and stems were 141.09,143.84,139.25,133.47 and 13.32, 9.85, 8.00, 4.65 mg/g, respectively. Among them, the content of Re, Rd and $Rb_2$ in flowers were more than 2/3 while the content of Re, $Rg_1$ and Rd in stems showed more than 9/10 amount of total saponin. The total saponin content of individual leaf were 19.46, 28.56, 58.82 and 169.24 mg/plant, 2.53, 2.76, 5.20 and 12.32 mg/plant in stems, and 14.11, 30.21, 37.60 and 73.41 mg/plant in flowers. Therefore, the total saponin content of aboveground parts in ginseng were leaves > flowers > stems.

Keywords

References

  1. An YN, Lee SY, Choung MG, Choi KJ and Kang KH. (2002). Ginsenoside concentration and chemical component as affected by harvesting time of four-year ginseng root. Korean Journal of Crop Science. 47:216-220
  2. Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L, Pugh W, Rue PA, Polonsky KS and Yuan CS. (2002). Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component. Diabetes. 51:1851-1858 https://doi.org/10.2337/diabetes.51.6.1851
  3. Chang HK. (1998). Changes of saponin contents in Panax ginseng leaves by different harvesting months. The Korean Journal of Food and Nutrition. 11:82-86
  4. Chang HK. (2003). Effect of processing methods on the saponin contents of Panax ginseng leaf-tea. The Korean Journal of Food and Nutrition. 16:46-53
  5. Cho SH. (1977). Saponins of Korean ginseng C. A. Meyer (Part II) The saponins of the ground part of ginseng. Journal of the Korean Agricultural Chemical Society. 20:142-146
  6. Corthout J, Naessens T, Apers S and Vlietinck AJ. (1999). Quantitative determination of ginsenosides from Panax ginseng roots and ginseng preparations by thin layer chromatographydensitometry. Journal of Pharmaceutical and Biomedical Analysis. 21:187-192 https://doi.org/10.1016/S0731-7085(99)00109-0
  7. Dey L, Xie JT, Wang A, Wu J, Maleckar SA and Yuan CS. (2003). Anti-hyperglycemic effects of ginseng: Comparison between root and berry. Phytomedicine. 10:600-605 https://doi.org/10.1078/094471103322331908
  8. Dey L, Zhang L and Yuan CS. (2002). Anti-diabetic and antiobese effects of ginseng berry extract: comparison between intraperitoneal and oral administrations. The American Journal of Chinese Medicine. 30:645-647 https://doi.org/10.1142/S0192415X02000648
  9. Han JH, Park SJ, Ahn CN, Wee JJ, Kim KY and Park SH. (2004). Nutritional composition, ginsenoside content and fundermental safety evaluation with leaf and stem extract of Panax ginseng. Journal of the Korean Society of Food Science and Nutrition. 33:778-784 https://doi.org/10.3746/jkfn.2004.33.5.778
  10. Hu JN, Lee JH, Shin JA, Choi JE and Lee KT. (2008). Determination of ginsenosides content in Korean ginseng seeds and roots by high performance liquid chromatography. Food Science and Biotechnology. 17:430-433
  11. Kim CH, Han JG, Jin L, Jung HS, Oh SH, Jeong MH, Jung KH, Choi GP, Park UY and Lee HY. (2009). Comparison of alcohol fermentation of low quality potatoes and sweet potatoes with ultrasonification process. Korean Journal of Medicinal Crop Science. 17:121-124
  12. Kim CH, Kwon MC, Han JG, Ha JH, Jeong HS, Choi GP, Park UY, Nam JH, Hwang B and Lee HY. 2008. Immune activities of Rhodiola sachalinensis A. Bor extracts isolated with various extraction process. Korean Journal of Medicinal Crop Science. 16:383-389
  13. Kim CS and Kim SB. (2001). Determination of ginseng saponins by reversed-phase high performance liquid chromatography. Korean Journal of Medicinal Crop Science. 9:21-25
  14. Kim GS, Hyun DY, Kim YO, Lee SW, Kim YC, Lee SE, Son YD, Lee MJ, Park CB, Park HK, Cha SW and Song KS. (2008). Extraction and preprocessing methods for ginsenosides analysis of Panax ginseng C. A. Mayer. Korean Journal of Medicinal Crop Science. 16:446-454
  15. Kim SC, Choi KJ, Ko SR and Joo HK. (1987). Content comparison of proximate compositions, various solvent extracts and saponins in root, leaf and stem of Panax ginseng. Korean Journal of Ginseng Science. 11:118-122
  16. Kwon MC, Han JG, Ha JH, Oh SH, Jin L, Jeong HS, Choi GP, Hwang B and Lee HY. (2008a). Immuno-regulatory effect on Centella asiatica L. Urban extraction solvent associated with ultrasonification process. Korean Journal of Medicinal Crop Science. 16:194-300
  17. Kwon MC, Han JG, Jeong HS, Qadir SA, Choi YB, Ko JR and Lee HY. (2008b). Enhancement of immune activities of Opuntia ficus-indica L. var. Miller by ultrasonification extraction process. Korean Journal of Medicinal Crop Science. 16:1-8
  18. Lee SW, Kang SW, Seong NS, Hyun GS, Hyun DY, Kim YC and Cha SW. (2004). Seasonal changes of growth and extract content of roots in Panax ginseng C. A. Meyer. Korean Journal of Medicinal Crop Science. 12:483-489
  19. Liu Y, Yang J and Cai Z. (2006). Chemical investigation on Sijunzi decoction and its two major herbs Panax ginseng and Glycyrrhiza uralensis by LC/MS/MS. Journal of Pharmaceutical & Biomedical Analysis. 41:1642-1647 https://doi.org/10.1016/j.jpba.2006.02.033
  20. Park MK, Park JH, Han SB, Shin YG and Park IH. (1996). High-performance liquid chromatographic analysis of ginseng saponins using evaporative light scattering detection. Journal of Chromatography A. 736:77-81 https://doi.org/10.1016/0021-9673(95)01323-7
  21. Shao CJ, Xu JD, Kasai R and Tanaka O. (1989). Saponins from flower-buds of Panax ginseng cultivated at Jilin, China. Chemical & Pharmaceutical Bulletin. 37:1934-1935 https://doi.org/10.1248/cpb.37.1934
  22. Shi W, Wang YT, Li J, Zhang HQ and Ding L. (2007). Investigation of ginsenosides in different parts and ages of Panax ginseng. Food Chemistry. 102:664-668 https://doi.org/10.1016/j.foodchem.2006.05.053
  23. Vanhaelen-Fastre RJ, Faes ML and Vanhaelen MH. (2000). High-performance thin-layer chromatographic determination of six major ginsenosides in Panax ginseng. Journal of Chromatography A. 868:269-276 https://doi.org/10.1016/S0021-9673(99)01253-4
  24. Wan JB, Yang FQ, Li SP, Wang YT and Cui XM. (2006). Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLCELSD. Journal of Pharmaceutical & Biomedical Analysis. 41:1596-1601 https://doi.org/10.1016/j.jpba.2006.01.058
  25. Xie JT, Mehendale SR, Li XM, Quigg R, Wang XY, Wang CZ, Wu JA, Aung HH, Rue PA, Bell GI and Yuan CS. (2005). Anti-diabetic effect of ginsenoside Re in ob/ob mice. Biochimica et Biophysica Acta. 1740:319-325 https://doi.org/10.1016/j.bbadis.2004.10.010
  26. Xie JT, Zhou YP, Dey L, Attele AS, Wu JA, Gu M, Polonsky KS and Yuan CS. (2002). Ginseng berry reduces blood glucose and body weight in db/db mice. Phytomedicine. 9:254-258 https://doi.org/10.1078/0944-7113-00106
  27. Yahara S, KaJi K and Tanaka O. (1979). Further study on dammarane-type saponins of roots, leaves, flower-buds, and fruits of Panax ginseng C. A. Meyer. Chemical and Pharmaceutical Bulletin. 271:88-92 https://doi.org/10.1248/cpb.27.88
  28. Yahara S, Matsuura K, Kasai R and Tanaka O. (1976a). Saponins of buds and flowers of Panax ginseng C. A. Meyer. (1). Isolation of ginsenoside-Rd, -Re, and -$Rg_{1}$. Chemical & Pharmaceutical Bulletin. 24:3212-3213 https://doi.org/10.1248/cpb.24.3212
  29. Yahara S, Tanaka O and Komori T. (1976b). Saponins of the leaves of Panax ginseng C. A. Meyer. Chemical & Pharmaceutical Bulletin. 24:2204-2208 https://doi.org/10.1248/cpb.24.2204
  30. Zhang S, Takeda T, Zhu T, Chen T, Yao X, Tanaka Y and Okihara Y. (1990). A new minor saponin from the leaves of Panax ginsenag. Planta Medica. 56:298-301 https://doi.org/10.1055/s-2006-960963
  31. Zhang SL, Yao XS, Chen YJ, Cui CB, Tezuka Y and Kikuchi T. (1989). Ginsenoside La, a novel saponin from the leaves of Panax ginseng. Chemical & Pharmaceutical Bulletin. 37:1966-1968 https://doi.org/10.1248/cpb.37.1966