황기(Astragalus membranaceus)의 박피 유무와 재배 년 수에 따른 항산화 활성 연구

Antioxidant Activities According To Peeling and Cultivated Years of Astragalus membranaceus Roots

  • 고은정 (강원대학교 한방바이오연구소) ;
  • 성은수 (강원대학교 한방바이오연구소) ;
  • 이재근 (강원대학교 한방바이오연구소) ;
  • 나종국 (미국 펜실베니아주립대학교 생물학과) ;
  • 임정대 (강원대학교 삼척캠퍼스 생약자원개발학과) ;
  • 김명조 (강원대학교 한방바이오연구소) ;
  • 김나영 (강원도 송호대학 외식조리과) ;
  • 이귀현 (강원대학교 한방바이오연구소) ;
  • 서정식 (강원도 농업기술원 북부농업시험장) ;
  • 최대성 (강원도 정선군 농업기술센터) ;
  • 정일민 (건국대학교 생명환경과학대학 응용생물과학과) ;
  • 유창연 (강원대학교 한방바이오연구소)
  • Goh, Eun-Jeong (Bioherb Research Institute, Kangwon National University) ;
  • Seong, Eun-Soo (Bioherb Research Institute, Kangwon National University) ;
  • Lee, Jae-Geun (Bioherb Research Institute, Kangwon National University) ;
  • Na, Jong-Kuk (Biology Department, 208 Mueller Laboratory, Penn State University) ;
  • Lim, Jung-Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Myong-Jo (Bioherb Research Institute, Kangwon National University) ;
  • Kim, Na-Young (Food Service Cuisine, Songho College) ;
  • Lee, Gwi-Hyun (Bioherb Research Institute, Kangwon National University) ;
  • Seo, Jung-Sik (Northern Agricultural Experimental Station, Gangwondo Agricultural Research and Extension Services) ;
  • Cheoi, Dae-Sung (Gangwondo Jeongseon Agricultural Extension Center) ;
  • Chung, Ill-Min (Department of Applied Life Science, College of Life and Environmental Sciences, Konkuk University) ;
  • Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University)
  • 발행 : 2009.08.30

초록

Astragalus membranaceus has a long history of medicinal use in Chinese herbal medicine. It has been shown to have immunostimulant, tonic, antioxidant, antiperspirant, diuretic, anti-diabetic, expectorant properties, and a supplementary medicine during cancer therapy. In this study, we investigated the effect of anti-oxidation of Astragalus membranaceus root extract. The anti-oxidative activities of water, 80% methanol, and 100% methanol extracts from Astragalus membranaceus were analyzed by DPPH free radical scavenging activity, Superoxide dismutase-like activity, reducing power, and crude ash. The water extract demonstrated to be more effective than methanol extract for a DPPH radicals scavenging activities and reducing power. Superoxide dismutase-like activity showed higher efficiency in 80% methanol extract. Our results indicate that Astragalus membranaceus extracts could be used as a source of antioxidant ingredients in the food industry.

키워드

참고문헌

  1. AOAC. (1990). Official methods of analysis. 15th edition Association of official analytical chemists, Washington, D.C., USA
  2. Hwang JB, Yang MO and Shin HK. (1997). Survey for approximate composition and mineral content of medicinal herbs. Korean Society Food Science and Technology. 29:671-679
  3. Jo C, Jeong IY, Kim DS, Son JH, An BJ, Choi JS and Byun MW. (2005). Comparison of the biological activity between a radiation-processed natural extract and a commercial counterpart for an industrial application. Food Engineering Progress. 9:177-181
  4. Jung C. (2005). Studies on immunomodulatory and antioxidant activities of Astragali membranacei Radix according to the cultivated years. Kyunghee University
  5. Kim HJ, Bae YC, Park RW, Choi SW, Cho SH, Choi YS and Lee WJ. (2002). Bone protecting effect of safflower seeds in ovariectomized rats. Calcified Tissue International. 71:88-94 https://doi.org/10.1007/s00223-001-1080-4
  6. Kim MJ, Kim JY, Choi SW, Hong JT and Yoon KS. (2004). Anti-wrinkle effect of safflower (Carthamus tinctorius L.) seed extract (I). The Society of Cosmetic Scientists Korea. 30:15-22
  7. Marklund S and Marklund G. (1974). Involvement of superoxide anion radical in autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 47:469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  8. Ma XQ, Duan JA, Zhu DY, Dong TTX and Tsim KWK. (2000). Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5S-rRNA spacer domain. Phytochemistry. 54:363-368 https://doi.org/10.1016/S0031-9422(00)00111-4
  9. Oraiza M. (1986). Studies on product of browning reaction prepared from glucosamine. Japanese Journal of Nutrition. 44:307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  10. Polovka M, Brezova V and Stasko A. (2003). Antioxidant properties of tea investigated by EPR spectroscopy. Biophysical Chemistry. 106:39-56 https://doi.org/10.1016/S0301-4622(03)00159-5
  11. Rios JL and Waterman PG. (1997). A review of the pharmacology and toxicology of Astragalus. Phytotheraphy Research. 11:411-418 https://doi.org/10.1002/(SICI)1099-1573(199709)11:6<411::AID-PTR132>3.0.CO;2-6
  12. Su MS, Shyu YT and Chien PJ. (2008). Antioxidant activities of citrus herbal product extracts. Food Chemistry. 111:892-896 https://doi.org/10.1016/j.foodchem.2008.05.002
  13. Xiong Q, Kadota S, Tani T and Namba T. (1996). Antioxidative effect of phenylethanoids from Cistanche deserticola. Biological and Pharmaceutical Bulletin. 19:1580-1585 https://doi.org/10.1248/bpb.19.1580
  14. Xie ZF. (1994). Classified dictionary of traditional Chinese medicine. New World Press, Beijing. p. 374
  15. Yin Y, Heo SI, Jung MJ and Wang MH. (2009). Antioxidant and antidiabetic effect of various sections of Astragalus membranaceus. The Korean Society of Pharmacognosy. 40:1-5